PENGARUH DOSIS DAN LAMA PEMBERIAN EKSTRAK DAUN ASAM JAWA (TAMARINDUS INDICA LINN) TERHADAP HOMA-Β PADA TIKUS MODEL DIABETES MELLITUS TIPE 2 The effect of Tamarind Leaf (tamarindus indica linn) Extract on HOMA-β in Rats with Type 2 Diabetes Mellitus Model

Devi Novia, Sugiarto Sugiarto, Yulia Lanti Dewi

= http://dx.doi.org/10.20473/mgi.v16i3.267-272

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Abstract


Nowadays the epidemiological burden of diabetes increases with long life-threatening symptoms and the effects      of antidiabetic drugs. Lack of insulin activity is one of the signs of a drop in diabetes mellitus. The mechanisms in antidiabetic include stimulating β-Langerhans cells which secrete insulin and inhibit enzyme activity. The purpose of this study was to analyze the effect of giving tamarind leaf extract on levels of homa-β in type 2 diabetes mellitus rats. This study used 30 male Wistar rats aged 8-12 weeks with a bodyweight of 150-200 grams and separated into 5 groups. The first group is KN group (DMT2 mice + standard diet), group 2 is KP (DMT2 + Acarbose mice), group 3 is P1 (DMT2 mice + tamarind leaf extract 28 mg / 200gr / day), group 4 is P2 (rat DMT2 + tamarind leaf extract 56 mg/200gr/day), and group 5 is P3 (DMT2 rat + tamarind leaf extract 112 mg / 200gr / day). The measurement method for Homa-β is to use a standardized formula and use the results of blood tests for fasting blood glucose and insulin levels. The results of the inter-variable study using one-way Anova found a significant difference between the levels of homa-β and the administration of tamarind leaves extract in rats with type 2 diabetes mellitus model (p <0.05). There were significant differences in the 5 treatment groups. On the 7th day, there was an increase in homa-β levels in the KP, P1, P2, and P3 groups while in the KN group decreased in homa-β levels. The P3 group was seen to have the highest increase in homa-β levels in the 14th day, but on the 14th day there was no significant difference between the acarbose drug group (99.57 ± 6.41) and the P3 group (15.09 ± 1, 71). The conclusion was the administration of tamarind extract at a dose of 28.56, and 112 mg/kgBW/day significantly increased levels of HOMA-β for 7 and 14 days in rats with type 2 diabetes mellitus.


Keywords


Type 2 Diabetes Mellitus, Tamarind Leaf Extract, Homa-β

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References


American Diabetes Association. (2011) Diagnosis and classification of diabetes mellitus. Diabetes Care. 34(suppl 1): S62–S69.

Babulreddy N, S.P. Sahoo, S. Ramachandran, M.D. Dhanaraju. (2013). Anti-Hyperglycemic Activity Of Cucum is Melo LeaffExtracts In Streptozotocin-Induced Hyperglycemia In Rats. International Journal Pharm Res Allied Science. 22-27.

Escalona Arranz, J. C., Perez Roses, R., Rodríguez Amado, J., Morris Quevedo, H. J., Mwasi, L. B., et al. (2015). Antioxidant and toxicological evaluation of Tamarindus indicaL. leafffluid extract. Natural Product Research, vol.30, no.4.

Forouhi NG, Koulman A, Sharp SJ, Imamura F, KrogerJJ, SchulzeMB., et al. (2014). Differencesin the prospective association between individual plasma phospholipid saturated fatty acids and incident type 2 diabetes: the EPIC InterAct case cohort study. The lancet Diabetes and endocrinology. 810-8.31.

Fumiaki Imamura, Kenneth J. Mukamal, James B. Meigs, Jose A. Luchsinger, Joachim H. et al. (2013). Risk Factors for Type 2 Diabetes Mellitus Preceeded by β-Cell Dysfunction, Insulin Resistance or Both in Older Adults. American Journal of Epidemiology. 177; 12.

Genova M P, K. Todorova-Ananieva, B. Atanasova and K. Tzatchev. (2014). Assessment of Beta Cell Function during pregnancy And after Delivery. Acta Medica Bulgarica. 41(1):5- 12(8).

Ghaffari MA., Payami SA., Payami Seyed- Peyman, Larky DA.,Nikzamir A., Ghorban MZ. (2016). Evaluation ofIaInsulin Resistance Indices in Types 2 Diabetic Patients Treated with Different AntiDiabetic Drugs. Journal of endocrine and Metabolic Diseases. 95

-101.

Haque N., Sofi G., Ali W., Rashid M., Itrat M. (2015). A Comprehensive Review of Phytochemical and Pharmacological Profile of Anar (Punica Granatum Linn ): A heaven’s fruit. Journal of ayurvedic and Herbal Medicine, vol.1, no.1.

Havulinna AS, Sysi-Aho M, Hilvo M, Kauhanen D, Hurme R, Ekroos K, et al. (2016).Circulating Ceramides Predict Cardiovascular Outcomes in the Population Based FINRISK 2002 Cohort. Arteriosclerosis, thrombosis, and vascular biology. 2424-2430.

Lemaitre RN, Fretts AM, Sitlani CM, Biggs ML, Mukamal K, King IB, et al. (2015) . Plasma phospholipid very long-chain saturated fatty acids and incident diabetes in older adults: the Cardiovascular Health Study. The American journal of clinical nutrition . 101: 1047-54.

Olfiana T. Lahamado, Sri M. Sabang, Dan Kasmudin Mustapa. (2017). Ekstrak Daun Asam Jawa (Tamarindus Indica L.) Sebagai Antidiabetes. Journal of Akademika Kimia. vol.6, no.1

Park S, M-Y Kim, S H Baik, J-T Woo, Y J Kwon, J W Daily. (2013) . Gestational diabetes is associated with high energy and saturated fat intakes and with low plasma Visfatin and adiponectin levels independent of pre-pregnancy BMI. European Journal of Clinical Nutrition. 67: 196-201. 42.

Raquel A , Laura , Gerardo Weisstaub, Lydia M , Cecilia BA., Estela Blanco. (2011).High HOMA-IR, adjusted for puberty, relates to the metabolic syndrome in overweight and obese children youths. Paediatr Diabetes. 212-8.

Shuang Zheng, Huan Zhou, Tingting Han, Yangxue Li, Yao Zhang, Wei Liu. ( 015). Clinical characteristics and beta-cell function in Chinese patients with newly diagnosed type 2 diabetes mellitus with different levels of serum triglyceride. BMCcEndocrine Disorders.15: 21.

Takumi Hirata, Aya Higashiyama, Yoshhimi Kubota, Kunihiro Nishimura, Daisuke Sugiyama, Aya Kodata. (2015). HOMA-IR Values are Associated With Glycemi Control in Japanese Subjects Without Diabetes or Obesity : The KOBE Study. Journal Epidemiol. 407- 414.

Ussher JR, Koves TR, Cadete VJ, Zhang L, Jaswal JS, Swyrd SJ, Lopaschuk DG, Proctor SD, Keung W, Muoio M and Lopaschuk GD. (2010). Inhibition of de novo ceramide synthes is reverses diet-induced insulin resistance and enhances whole-body oxygen consumption. Journal of Diabetes. 59:2453-64.

Yu X, Chen P,Wang H, Jin H, JiaW,Wang L. (2014). Clinical study of exercise on improvement of β-Cell unction and insulin resistance in non-diabetic young off springs of diabetic patients. Journal of Endocrinological investigation. 37(4):353-358.


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