The effects of topical application of red pomegranate (Punica granatum Linn) extract gel on the healing process of traumatic ulcers in Wistar rats

Sri Hernawati, Yonanda Az Zikra, Dwi Warna Aju Fatmawati

= http://dx.doi.org/10.20473/j.djmkg.v52.i2.p90-94
Abstract views = 53 times | views = 27 times

Abstract


Background: Trauma-induced ulcers constitute one of the painful abnormalities affecting the oral cavity about which numerous individuals complain. The prevalence of traumatic ulcers (15-30% of cases) is relatively high compared to other oral lesions. Unfortunately, the use of anti-inflammatory drugs classified as steroids can lead to contraindications and serious side effects. Hence, the use of natural ingredients represents an alternative treatment. One such ingredient is red pomegranate containing bioactive elements acting as anti-inflammatory, antibacterial, antimicrobial, and antioxidant agents which accelerate the healing process in traumatic ulcers. Purpose: The aim of this study was to determine the effects of the topical application of red pomegranate (Punica granatum Linn) extract on the healing process in traumatic ulcers in Wistar rats. Methods: 24 male rats, aged 2-3 months, were divided into six groups, namely; two control groups (PC: triamcinolone acetonide and NC: sterile aquades) and four treatment groups (red pomegranate extract gel at respective concentrations of 12.5%, 25%, 50% and 75%). A traumatic ulcer was subsequently created using a flame-shaped burnisher tip 2mm in diameter and heated for ±15 seconds. The diameter traumatic ulcer of each research subject was measured and observed until it healed within the remedial parameters determined by means of a UNC-15 periodontal probe which had been placed on it. The data obtained was analyzed using Kruskal-Wallis and Mann-Whitney tests. Results: Topically applied red pomegranate extract gel can reduce the width of the ulcerated area, while also accelerating traumatic ulcer healing. There was a significant difference in the healing time between the group using red pomegranate extract gel at a concentration of 75% and the positive control group using 0.1% triamcinolone acetonide with p=0.44 (p<0.05). Conclusion: Red pomegranate extract gel possesses the ability to accelerate the healing process in traumatic ulcers. The most effective concentration of red pomegranate extract gel at accelerating the healing process in traumatic ulcers is 75%.

Keywords


anti-inflammatory; antioxidant; red pomegranate; traumatic ulcer

Full Text:

PDF

References


Sunarjo L, Hendari R, Rimbyastuti H. Manfaat xanthone terhadap kesembuhan ulkus rongga mulut dilihat dari jumlah sel PMN dan fibroblast. ODONTO Dent J. 2015; 2(2): 14–21.

Langlais RP, Miller CS, Nield-Gehrig JS. Atlas berwarna: Lesi mulut yang sering ditemukan. 4th ed. Jakarta: EGC; 2014. p. 172–3.

Langkir A, Pangemanan DHC, Mintjelungan CN. Gambaran lesi traumatik mukosa mulut pada lansia pengguna gigi tiruan sebagian lepasan di panti werda kabupaten Minahasa. e-GIGI. 2015; 3: 1–8.

Skidmore-Roth L. Mosby’s 2014 nursing drug reference. 27th ed. Missouri: Mosby Elsevier; 2014. p. 40–3.

Budi DTW. Efek salep ekstrak herba meniran (Phyllanthus Niruri L.) dibandingkan dengan Triamcinolone Acetonide 0.1% terhadap proses penyembuhan luka sayat mukosa rongga mulut tikus Wistar jantan secara histopatologi. Thesis. Bandung: Universitas Kristen Maranatha; 2017. p. 1–3.

Kshitiz P, Shipra Z, Rani S, Jayanti S. Anti-cariogenic effects of polyphenol plant products-a review. Int J Res Ayurveda Pharm. 2011; 2(3): 736–42.

Hernawati S. Ekstrak buah delima sebagai alternatif terapi recurrent apthous stomatitis (RAS). Stomatognaic - J Kedokt Gigi. 2015; 12: 20–5.

Setiadhi R, Sufiawati I, Zakiawati D, Nur’aeny N, Hidayat W, Firman DR. Evaluation of antibacterial activity and acute toxicity of pomegranate (punica granatum l.) seed ethanolic extracts in swiss webster mice. J Dentomaxillofacial Sci. 2017; 2(2): 119–23.

Mujayanto R. Pengaruh zinc sulfat 1% topikal terhadap jumlah makrofag ulkus traumatikus mulut tikus Wistar diabetes. Thesis. Surabaya: Universitas Airlangga; 2016. p. 30–40.

Mara RM. Pengaruh ekstrak propolis terhadap penyembuhan ulkus traumatik pada mukosa oral. Thesis. Banda Aceh: Universitas Syiah Kuala; 2014. p. 40–54.

drugs.com. Kenalog Orabase - FDA prescribing information, side effects and uses. 2018. Available from: https://www.drugs.com/pro/kenalog-orabase.html. Accessed 2018 Jul 18.

Pramono A, Mayasari LO. Efektifitas pemberian ekstrak gel belimbing manis (Averrhoa carambola Linn) terhadap kesembuhan ulkus traumatikus studi in vivo terhadap mukosa tikus (Strain Wistar). In: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat. Semarang: Universitas Muhammadiyah Semarang; 2017. p. 39–44.

Zhang Y, Krueger D, Durst R, Lee R, Wang D, Seeram N, Heber D. International multidimensional authenticity specification (IMAS) algorithm for detection of commercial pomegranate juice adulteration. J Agric Food Chem. 2009; 57(6): 2550–7.

Rahmina P. Pengaruh ekstrak daun pacar kuku (Lawsonia Inermis) 7,5% terhadap penyembuhan ulkus traumatik pada mukosa oral (penelitian ada tikus odel). Thesis. Banda Aceh: Universitas Syiah Kuala; 2013. p. 62.

Istiana S. Formulasi sediaan gel basis CMC-Na ekstrak etanol daun cocor bebek (Kalanchoe pinnata (Lmk.) Pers.) sebagai penyembuh luka bakar pada kelinci. Thesis. Surakarta: Universitas Muhammadiyah Surakarta; 2016. p. 1–12.


Refbacks

  • There are currently no refbacks.


View My Stats