The potential effect of Moringa oleifera leaves extract on vascular endothelial growth factor expression in Wistar rat oral cancer cells

Dwicha Rahma Nuriska Hartono, Theresia Indah Budhy Sulisetyawati, Edhi Jularso

= http://dx.doi.org/10.20473/j.djmkg.v52.i2.p71-75
Abstract views = 125 times | views = 56 times

Abstract


Background: Cancer is a disease characterized by abnormal and uncontrolled growth of tissue cells. In Indonesia, cancer ranks as the fifth largest cause of mortality, while it is the second largest worldwide. Cancer affected by angiogenesis, the process of forming new blood vessels to provide the nutrient and oxygen necessary for tumor growth. Vascular endothelial growth factor (VEGF) represents a pro-angiogenic factor. Moringa oleifera leaf extract can be used as an anticancer agent by reducing the expression of VEGF. Purpose: The study aimed to prove that Moringa oleifera leaf extract can reduce VEGF expression in benzopyrene-induced oral cancer cells of Wistar rats. Methods: This was an experimental laboratory research with posttest-only control group design. All experimental subjects presented symptoms of cancer following induction with 8 mg/KgBW of benzopyrene. The sample consisted of 28 Wistar rats, divided into four groups, namely; a control group (K) whose members were administered with only aquadest and three treatment groups (P) treated with Moringa oleifera leaves extract at percentages of 3.125% (P1), 4.6875% (P2) and 6.25% (P3) respectively. Observation of VEGF expression was undertaken by means of immunohistochemical staining. Results: A decrease in VEGF expression occurred in all treatment groups when compared with the control group. A significant difference existed between the control group (K) and the treatment group (P1), while there were no significant differences between the treatment groups (P1, P2 and P3). Conclusion: Moringa oleifera leaf extract with a percentage of 3.125% proved most effective at reducing VEGF expression in oral cancer cells in Wistar rats.

Keywords


isothiocyanate; Moringa oleifera extract; oral cancer; VEGF expression

Full Text:

PDF

References


Epstein J, Elad S. Oral and oropharyngeal cancer. In: Glick M, editor. Burket’s oral medicine. 12th ed. Raleigh: People’s Medical Publishing House-USA; 2015. p. 173–99.

Aulawi T. Hubungan konsumsi daging merah dan gaya hidup terhadap risiko kanker kolon. Kutubkhanah. 2013; 16: 37–45.

Gupta P, Kim B, Kim S-H, Srivastava SK. Molecular targets of isothiocyanates in cancer: recent advances. Mol Nutr Food Res. 2014; 58(8): 1685–707.

Abdul-Aziz MA, Amin AK, El-Rouby DH, Shaker OG. Lymphangiogenesis in oral squamous cell carcinoma: correlation with VEGF-C expression and lymph node metastasis. Int J Dent. 2017; 2017: 1–10.

Kresno SB. Ilmu dasar onkologi. 3rd ed. Jakarta: Badan Penerbit Fakultas Kedokteran Universitas Indonesia; 2011. p. 344–67.

Niu G, Chen X. Vascular endothelial growth factor as an anti-angiogenic target for cancer therapy. Curr Drug Targets. 2010; 11(8): 1000–17.

Mărgăritescu C, Pirici D, Stîngă A, Simionescu C, Raica M, Mogoantă L, Stepan A, Ribatti D. VEGF expression and angiogenesis in oral squamous cell carcinoma: an immunohistochemical and morphometric study. Clin Exp Med. 2010; 10(4): 209–14.

Ahimsa GK. Aktivitas antikanker ekstrak kelor (Moringa oleifera L.) terhadap cell line kanker serviks hela dengan uji sitotoksisitas, apoptosis, dan jalur induksi apoptosis berdasarkan ekspresi gen p53. Thesis. Yogyakarta: Univeritas Gadjah Mada; 2014. p. 1–13.

Loprinzi C, Bensinger W, Peterson D. Understanding and managing chemotherapy side effects. New Jersey: Cancer Care; 2014. p. 4–7.

Chu E, Devita Jr VT. Physicians’ cancer chemotherapy: drug manual 2015. 15th ed. Vol. 111, The British Journal of Psychiatry. Burlington: Jones & Bartlett Learning; 2015. p. 4.

Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: a food plant with multiple medicinal uses. Phyther Res. 2007; 21(1): 17–25.

Cavell BE, Syed Alwi SS, Donlevy A, Packham G. Anti-angiogenic effects of dietary isothiocyanates: mechanisms of action and implications for human health. Biochem Pharmacol. 2011; 81(3): 327–36.

Nussenbaum F, Herman IM. Tumor angiogenesis: insights and innovations. J Oncol. 2010; 2010: 1–24.

Boreddy SR, Sahu RP, Srivastava SK. Benzyl isothiocyanate suppresses pancreatic tumor angiogenesis and invasion by inhibiting HIF-α/VEGF/Rho-GTPases: pivotal role of STAT-3. PLoS One. 2011; 6(10): e25799.

Nararya SA. Uji toksisitas daun kelor (Moringa Oleifera) terhadap sel fibroblas gingiva menggunakan uji MTT assay. Thesis. Surabaya: Universitas Airlangga; 2018. p. 1–7.

Budhy TI, Istiati, Sumaryono B, Arundina I, Khrisnanthi RS. Hedyotiscorymbosa (L.) Lamk - the potential inhibitor extract of oral cancer cell progressivity in benzopyrene induced rattus novergicus. J Int Dent Med Res. 2018; 11: 312–7.

Anggraini DR. Ekspresi imunohistokimia Ki-67 pada tumor payudara tikus Wistar yang diinokulasi kanker terinduksi Benzo(α)pyrene dengan pemberian ekstrak benalu teh. Thesis. Medan: Universitas Sumatera Utara; 2013. p. 15.

Juliyarsi I, Melia S. Dadih susu sapi mutan lactococcus lactis sebagai food healthy dalam menghambat kanker. Padang; 2007. Report No.: 001/SP2H/PP/DP2M/III/2007.

Elisabeth HJT, Donald S. Polycyclic aromatic hydrocarbon (PAH): kaitannya dengan minyak sawit dan kesehatan. War Pus Penelit Kelapa Sawit. 2000; 8(1): 1–9.

Ibrahim S, Marham S. Teknik laboratorium kimia organik. Yogyakarta: Graha Ilmu; 2013. p. 16.

Koirewoa YA, Fatimawali F, Wiyono W. Isolasi dan identifikasi senyawa flavonoid dalam daun beluntas (Pluchea indica L.). Pharmacon. 2012; 1(1): 47–52.

Yulianingtyas A, Kusmartono B. Optimasi volume pelarut dan waktu maserasi pengambilan flavonoid daun belimbing wuluh (Averrhoa Bilimbi L.). J Tek Kim. 2016; 10(2): 58–64.

Rizkayanti R, Diah AWM, Jura MR. Uji aktivitas antioksidan ekstrak air dan ekstrak etanol daun kelor (Moringa Oleifera LAM). J Akad Kim. 2017; 6(2): 125–31.

Vongsak B, Sithisarn P, Mangmool S, Thongpraditchote S, Wongkrajang Y, Gritsanapan W. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind Crops Prod. 2013; 44: 566–71.

Fahey JW, Zalcmann AT, Talalay P. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry. 2001; 56(1): 5–51.

Kerbel RS. Tumor angiogenesis. N Engl J Med. 2008; 358(19): 2039–49.

Kim S-K, Park S-G, Kim K-W. Expression of vascular endothelial growth factor in oral squamous cell carcinoma. J Korean Assoc Oral Maxillofac Surg. 2015; 41: 11–8.

Lalla R V., Boisoneau DS, Spiro JD, Kreutzer DL. Expression of vascular endothelial growth factor receptors on tumor cells in head and neck squamous cell carcinoma. Arch Otolaryngol Neck Surg. 2003; 129(8): 882–8.

Smith BD, Smith GL, Carter D, Sasaki CT, Haffty BG. Prognostic significance of vascular endothelial growth factor protein levels in oral and oropharyngeal squamous cell carcinoma. J Clin Oncol. 2000; 18(10): 2046–52.

Hakim L. Farmakokinetik klinik. Yogyakarta: Bursa Ilmu; 2012. p. 216–9.

Deng L, Lin-Lee Y-C, Claret F-X, Kuo MT. 2-acetylaminofluorene up-regulates rat mdr1b expression through generating reactive oxygen species that activate NF-kappa B pathway. J Biol Chem. 2001; 276: 413–20.

Matheny CJ, Lamb MW, Brouwer KLR, Pollack GM. Pharmacokinetic and pharmacodynamic implications of P-glycoprotein modulation. Pharmacotherapy. 2001; 21(7): 778–96.

Marchetti S, Mazzanti R, Beijnen JH, Schellens JHM. Concise review: Clinical relevance of drug drug and herb drug interactions mediated by the ABC transporter ABCB1 (MDR1, P-glycoprotein). Oncologist. 2007; 12(8): 927–41.

Nugrahaningsih, Sarjadi, Dharmana E, Subagio HW. Ekspresi VEGF sel Adenokarsinoma mamma pada pemberian oral ekstrak Andrographis paniculata. J Ilmu Kefarmasian Indones. 2015; 13: 29–34.

Kumar C, Kohli S, Bapsy PP, Vaid AK, Jain M, Attili VSS, Sharan B. Immune modulation by dendritic-cell-based cancer vaccines. J Biosci. 2017; 42(1): 161–73.


Refbacks

  • There are currently no refbacks.


View My Stats