The enhancement of type 1 collagen expression after 10% propolis-carbonated hydroxyapatite application in periodontitis-induced rabbits

Indi Kusumawati, S. Suryono, Ahmad Syaify

= http://dx.doi.org/10.20473/j.djmkg.v54.i1.p16-20
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Abstract


Background: Alveolar bone defects caused by periodontitis may require regenerative therapy to restore bone structure. Propolis possesses antibacterial, anti-inflammatory and antioxidant properties, which can stimulate bone regeneration. Propolis-carbonated hydroxyapatite (CHA) material was applied during open flap debridement (OFD) in periodontitis therapy. Purpose: To analyse the effect of the application of 10% propolis-CHA after OFD on type 1 collagen expression on periodontitis-induced rabbits. Methods: Six male rabbits, aged 5–8 months, weight 1500–2000 grams, were ligated with wire and injected with Porphyromonas gingivalis lipopolysaccharide (LPS) for six weeks to induce periodontitis. The samples were divided into three groups: group A (OFD only), group B (OFD+CHA) and group C (OFD+10% propolis-CHA). To acquire 10% propolis-CHA, the CHA block was cut into 10mg and then immersed in 1ml of 10% propolis solution for 24 hours at room temperature. Decapitation was performed on the seventh and 14th day after OFD was performed on each group and microscopic slides were prepared for type 1 collagen examination. The data was analysed using a two-way ANOVA with a 95% confidence interval followed by a post hoc LSD test. Results: The type 1 collagen expression in group C (61.36±1.88 on day seven and 70.25±3.89 on day 14) was significantly different from group A (42.91±1.78 on day seven and 45.18±2.48 on day 14) and group B (43.91±5.31 on day seven and 59.63±3.27 on day 14) on both the seventh day and the 14th day (p=0.000). Conclusion: The administration of 10% propolis-CHA during OFD can increase the type 1 collagen expression in the alveolar bone of rabbits on the seventh and 14th day.

Keywords


carbonated hydroxyapatite; periodontitis; propolis; type 1 collagen

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References


Carranzza, F.A., Newman, M.G., Takei, H.H. dan KPR. Carranza’s clinical periodontology. 11th ed. St Louis Missouri: Saunders Elsevier; 2012. p. 62–3.

Nishihara T, Koseki T. Microbial etiology of periodontitis. Periodontol 2000. 2004; 36: 14–26.

Graves DT, Kang J, Andriankaja O, Wada K, Rossa C. Animal models to study host-bacteria interactions involved in periodontitis. Front Oral Biol. 2012; 15: 117–32.

Crea A, Deli G, Littarru C, Lajolo C, Orgeas GV, Tatakis DN. Intrabony defects, open-flap debridement, and decortication: a randomized clinical trial. J Periodontol. 2014; 85: 34–42.

Muthu J, Namasivayam A, Perumalsamy R, Pushparajan S, Bose B, Kannan A. Efficacy of combination therapy using anorganic bovine bone graft with resorbable GTR membrane vs. open flap debridement alone in the management of grade II furcation defects in mandibular molars - A comparative study. J Int Soc Prev Community Dent. 2014; 4(4): 38.

Kaushal S. Evaluation of OSSIFI ® as alloplastic bone graft material in treatment of periodontal infrabony defects. J Clin Diagnostic Res. 2014; 8(10): 61–5.

Landi E, Celotti G, Logroscino G, Tampieri A. Carbonated hydroxyapatite as bone substitute. J Eur Ceram Soc. 2003; 23(15): 2931–7.

Lara Pereira YC, Mardegan Issa JP, Watanabe E, Nascimento GC, Iyomasa MM, Del Ciampo JO, Ervolino E. The therapeutic use of propolis extract in alveolar bone contaminated with bacterial endotoxin. Dentistry. 2018; 8(3): 1–9.

Zohery AA, Meshri SM, Madi MI, Abd El Rehim SS, Nour ZM. Egyptian propolis compared to nanohydroxyapatite graft in the treatment of Class II furcation defects in dogs. J Periodontol. 2018; 89(11): 1340–50.

Suryono S, Hasmy NS, Pertiwi TL, Benyamin B. Propolis 10%-gel as a topical drug candidate on gingivitis. Int J Med Pharm. 2017; 5: 12–7.

Kusumawati I, Suryono, Syaify A. Loading and release profile assay of carbonated hydroxyapatite incorporated with propolis as bone graft material. Maj Obat Tradis. 2020; 25(2): 121–5.

Wijayanti P, Lastianny SP, Suryono S. Growth of NIH 3T3 fibroblast cells exposed to carbonated hydroxyapatite with incorporated propolis. Indones J Cancer Chemoprevention. 2020; 11(2): 54–9.

Devitaningtyas N, Syaify A, Herawati D, Suryono S. Evaluation of antibacterial potential of carbonated hydroxyapatite combined with propolis on porphyromonas gingivalis. Maj Obat Tradis. 2020; 25(1): 55–8.

Abdellatif B, Mohamed H, Karim A, Asma B. Radiography monitoring of osteoconduction and osteoinduction of orthotopic allograft autoclaved covered with propolis. Int J Adv Life Sci Technol. 2014; 1(1): 25–31.

Burr DB, Allen MR. Basic and Applied Bone Biology. Oxford: Elsevier Inc.; 2014. p. 55–6.

Neve A, Corrado A, Cantatore FP. Osteoblast physiology in normal and pathological conditions. Cell Tissue Res. 2011; 343(2): 289–302.

Vieira AE, Repeke CE, De Barros Ferreira S, Colavite PM, Biguetti CC, Oliveira RC, Assis GF, Taga R, Trombone APF, Garlet GP. Intramembranous bone healing process subsequent to tooth extraction in mice: micro-computed tomography, histomorphometric and molecular characterization. PLoS One. 2015; 10(5): 1–22.

Itagaki T, Honma T, Takahashi I, Echigo S, Sasano Y. Quantitative analysis and localization of mRNA transcripts of type I collagen, osteocalcin, MMP 2, MMP 8, and MMP 13 during bone healing in a rat calvarial experimental defect model. Anat Rec Adv Integr Anat Evol Biol. 2008; 291(8): 1038–46.

Zenobia C, Hasturk H, Nguyen D, Van Dyke TE, Kantarci A, Darveau RP. Porphyromonas gingivalis lipid A phosphatase activity is critical for colonization and increasing the commensal load in the rabbit ligature model. Bäumler AJ, editor. Infect Immun. 2014; 82(2): 650–9.

Zhang W, Ju J, Rigney T, Tribble G. Porphyromonas gingivalis infection increases osteoclastic bone resorption and osteoblastic bone formation in a periodontitis mouse model. BMC Oral Health. 2014; 14: 1–9.

Kruger TE, Miller AH, Wang J. Collagen scaffolds in bone sialoprotein-mediated bone regeneration. Sci World J. 2013; 2013: 1–6.

Fawcett DW. Buku ajar histologi (terj). 12th ed. Jakarta: EGC; 2002. p. 174–195.

Srivastava S, Bankar R, Roy P. Assessment of the role of flavonoids for inducing osteoblast differentiation in isolated mouse bone marrow derived mesenchymal stem cells. Phytomedicine. 2013; 20(8–9): 683–90.

Nakashima K, de Crombrugghe B. Transcriptional mechanisms in osteoblast differentiation and bone formation. Trends Genet. 2003; 19(8): 458–66.

Torre E. Molecular signaling mechanisms behind polyphenol-induced bone anabolism. Phytochem Rev. 2017; 16(6): 1183–226.


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