The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction
Downloads
Background: Research has proved that Aloe vera has anti-inflammatory, anti-bacterial, and immunomodulator properties that can accelerate the healing process. These properties could be beneficial in the tooth extraction wound-healing process. Neutrophils are the first inflammatory cells to invade and are the predominant cell markers in the wound for 24 hours after injury. Polymorphonuclear leukocytes (PMN) provide initial protection against micro-organisms, as they engulf and digest foreign bodies. Purpose: The aim of this study was to prove the effect of Aloe vera 90% gel application on the PMN count in the healing process of Cavia cobaya following tooth extraction. Methods: This laboratory experimental research was done using Post-Test Only Control Group Design. The lower incisive of twelve male Cavia cobayas were extracted then divided into four groups. Group P1 and P3 had 90% Aloe vera gel applied into the socket and sutured; group K1 and K3, received no 90% Aloe vera gel application and acted as controls. Samples from group P1 and K1 were terminated and had their mandibula taken one day after tooth extraction, and those from group P3 and K3 were terminated after three days. Tissues were then processed into histology slides, and PMN cells were counted. Results: There were significant differences in the PMN count between groups K1, P1, and K3 compared with P3 (p < 0.05). Higher PMN count was shown on the group containing Aloe vera 90% gel compared with control group, both on day one and three after tooth extraction. Conclusion: Aloe vera 90% gel can increase the PMN count in the inflammation phase of the tooth extraction wound-healing process.
Downloads
Nimma VL, Talla HV, Bairi JK, Gopaldas M, Bathula H, Vangdoth S. Holistic healing through herbs: effectiveness of Aloe vera on post extraction socket healing. J Clin Diagnostic Res. 2017; 11(3): ZC83–6.
Gonzalez AC de O, Costa TF, Andrade Z de A, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016; 91(5): 614–20.
Wang J. Neutrophils in tissue injury and repair. Cell Tissue Res. 2018; 371(3): 531–9.
Nanci A. Ten Cate's oral histology: development, structure, and function. 8th ed. St Louis: Mosby Elsevier; 2012. p. 379–83, 388–9.
Hajishengallis E, Hajishengallis G. Neutrophil homeostasis and periodontal health in children and adults. J Dent Res. 2014; 93(3): 231–7.
Phillipson M, Kubes P. The healing power of neutrophils. Trends Immunol. 2019; 40(7): 635–47.
Sumarta NPM, Danudiningrat CP, Rachmat EA, Soesilawati P. Cytotoxicity difference of 316L stainless steel and titanium reconstruction plate. Dent J (Majalah Kedokt Gigi). 2011; 44(1): 7–11.
Hashemi SA, Madani SA, Abediankenari S. The review on properties of Aloe vera in healing of cutaneous wounds. Biomed Res Int. 2015; 2015: 714216.
Cock IE. Problems of reproducibility and efficacy of bioassays using crude extracts, with reference to Aloe vera. Pharmacogn Commun. 2011; 1(1): 52–62.
Sierra-García GD, Castro-Ríos R, González-Horta A, Lara-Arias J, Chávez-Montes A. Acemannan, an extracted polysaccharide from Aloe vera: A literature review. Nat Prod Commun. 2014; 9(8): 1217–21.
Yuliati, Kusumaningsih T, Rananda I, Soesilawati P. Increasing macrophages in tooth extraction wound healing after induction of freeze-drying gel Aloe vera 90% on Cavia cobaya. Malaysian J Med Heal Sci. 2020; 16(4): 87–91.
Olympus Corporation. User manual cellSens 1.18 life science imaging software. Tokyo: OLYMPUS; 2017. p. 7–39, 139–75.
Cross SS. Underwood's pathology: a clinical approach. 6th ed. China: Churchill Livingstone Elsevier; 2013. p. 179–80.
Reinke JM, Sorg H. Wound repair and regeneration. Eur Surg Res. 2012; 49(1): 35–43.
Wilkinson HN, Hardman MJ. Wound healing: cellular mechanisms and pathological outcomes. Open Biol. 2020; 10(9): 200223.
Abdel Hamid AA, Soliman MF. Effect of topical aloe vera on the process of healing of full-thickness skin burn: a histological and immunohistochemical study. J Histol Histopathol. 2015; 2: 3.
Francisco-Cruz A, Aguilar-Santelises M, Ramos-Espinosa O, Mata-Espinosa D, Marquina-Castillo B, Barrios-Payan J, Hernandez-Pando R. Granulocyte-macrophage colony-stimulating factor: not just another haematopoietic growth factor. Med Oncol. 2014; 31(1): 774.
Jamil M, Mansoor M, Latif N, Naz R, Anwar F, Arshad M, Gul J, Ullah S, Saddam M. Review: Effect of Aloe vera on wound healing. Pakistan J Sci Ind Res. 2020; 63(1): 48–61.
Kamadjaja DB, Harijadi A, Soesilawati P, Wahyuni E, Maulidah N, Fauzi A, Rah Ayu F, Simanjuntak R, Soesanto R, Asmara D, Rizqiawan A, Agus P, Pramono C. Demineralized freeze-dried bovine cortical bone: Its potential for guided bone regeneration membrane. Int J Dent. 2017; 2017: 5149675.
Haque N, Widera D, Govindasamy V, Soesilawati P, Abu Kasim NH. Extracellular vesicles from stem and progenitor cells for cell-free regenerative therapy. Curr Mol Med. 2021; 21.
Gupta VK, Malhotra S. Pharmacological attribute of Aloe vera: Revalidation through experimental and clinical studies. Ayu. 2012; 33(2): 193–6.
Choche T, Shende S, Kadu P. Extraction and identification of bioactive components from Aloe barbadensis. J Pharmacogn Phytochem. 2014; 2(1): 14–23.
- Every manuscript submitted to must observe the policy and terms set by the Dental Journal (Majalah Kedokteran Gigi).
- Publication rights to manuscript content published by the Dental Journal (Majalah Kedokteran Gigi) is owned by the journal with the consent and approval of the author(s) concerned.
- Full texts of electronically published manuscripts can be accessed free of charge and used according to the license shown below.
- The Dental Journal (Majalah Kedokteran Gigi) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
