Cytotoxic test of different solvents of soursop (Annona muricata) leaf extract against HSC-3 cell line
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Background: Soursop (Annona muricata) leaves have been researched extensively and found to have anticancer properties. The use of soursop as an anticancer treatment is increasingly popular due to its selective cytotoxic activity by acetogenins. The polarity of the extract solvent contributes to the biological activity of the plant, namely cytotoxicity. Purpose: To determine the cytotoxicity of A. muricata leaf extract with ethanol, ethyl acetate and hexane fractions against human oral squamous carcinoma (HSC-3) cell lines. Methods: This experimental laboratory study consisted of twenty four treatment groups tested against the HSC-3 cell line. The ethanol, ethyl acetate and hexane fractions of A. muricata leaves were administered to seven different concentrations, namely 0.3 μg/mL, 3 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL, 150 μg/mL and 300 μg/mL. The control group consisted of three groups: negative control, solvent control and positive control. The percentage of cell viability was calculated by absorbent enzyme-linked immunosorbent assay (ELISA) reader. The cytotoxicity of A. muricata leaf extract against HSC-3 cells was determined by cell counting kit-8 (CCK-8) assay and expressed by IC50 value. The results were analysed using one-way analysis of variance (ANOVA) followed by Tukey's honestly significant difference (HSD). Results: The results show that the leaf extracts of A. muricata are moderately cytotoxic to HSC-3 cells. The highest cytotoxic activity was found in the ethyl acetate extract with an IC50 value of 76.66 μg/mL – making it the best solvent – then hexane (IC50: 84.14 μg), then ethanol (IC50: 101.32 μg/mL). Statistical analysis using one-way ANOVA and Tukey's HSD is considered significant p < 0.001. Conclusion: Ethanol, ethyl acetate and hexane fractions of A. muricata leaf extract are moderately cytotoxic, with IC50 values in the range of 21–200 μg/mL.
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Mathur G, Nain S, Sharma P. Cancer: An overview. Acad J Cancer Res. 2015; 8(1): 1–9. pdf: https://www.idosi.org/ajcr/8(1)15/1.pdf
Rivera C. Essentials of oral cancer. Int J Clin Exp Pathol. 2015; 8(9): 11884–94. pubmed: https://pubmed.ncbi.nlm.nih.gov/26617944/
Sirait AM. Faktor risiko tumor/kanker rongga mulut dan tenggorokan di Indonesia (analisis riskesdas 2007). Media Litbangkes. 2013; 23(3): 122–9. web: http://perpustakaan.litbang.kemkes.go.id/index.php?p=show_detail&id=37432
Pires FR, Ramos AB, de Oliveira JBC, Tavares AS, da Luz PSR, dos Santos TCRB. Oral squamous cell carcinoma: clinicopathological features from 346 cases from a single oral pathology service during an 8-year period. J Appl Oral Sci. 2013; 21(5): 460–7. doi: https://doi.org/10.1590/1679-775720130317
Gharat SA, Momin M, Bhavsar C. Oral squamous cell carcinoma: current treatment strategies and nanotechnology-based approaches for prevention and therapy. Crit Rev Ther Drug Carrier Syst. 2016; 33(4): 363–400. doi: https://doi.org/10.1615/CritRevTherDrugCarrierSyst.2016016272
Vinken M, Rogiers V. Protocols in in vitro hepatocyte research. Vinken M, Rogiers V, editors. New York: Springer; 2015. p. 1–390. (Methods in Molecular Biology; vol. 1250). web: http://link.springer.com/10.1007/978-1-4939-2074-7
Zafrial RM, Amalia R. Anti kanker dari tanaman herbal. Farmaka. 2018; 16(1): 15–6. web: https://jurnal.unpad.ac.id/farmaka/article/view/17332
Lestari R, Solihah SM, Aprilianti P, Wawangningrum SHH, Agustin EK, Sahromi, Wibowo ARU, Munawaroh S, Permatasari PA. Koleksi tumbuhan buah kebun raya Katingan. Witono JR, Yuzammi, editors. Jakarta: LIPI Press; 2017. p. 28–9. web: https://lipipress.lipi.go.id/detailpost/koleksi-tumbuhan-buah-kebun-raya-katingan
Widyastuti DA, Rahayu P. Antioxidant capacity comparison of ethanolic extract of Soursop (Annona muricata Linn.) leaves and seeds as cancer prevention candidate. Biol Med Nat Prod Chem. 2017; 6(1): 1–4. doi: https://doi.org/10.14421/biomedich.2017.61.1-4
Fertilita S, Sandhika W, Suprabawati DGA. The cytotoxic activity of Annona muricata Linn leaves ethanolic extract (AMEE) on T47D breast cancer cell line. Med Lab Technol J. 2020; 1(1): 32–9. doi: https://doi.org/10.31964/mltj.v1i1.291
Indrawati L, Pramono S, Ascobat P, Bela B, Abdullah M, Surono IS. Cytotoxic activity of soursop "Annona muricata” leaves extracts and their phytochemical contents. J Glob Pharma Technol. 2017; 9(2): 35–40. web: http://www.jgpt.co.in/index.php/jgpt/article/view/105
Qorina F, Arsianti A, Fithrotunnisa Q, Tejaputri NA, Azizah NN, Putrianingsih R. Cytotoxicity of soursop leaves (Annona muricata) against cervical HeLa cancer cells. Pharmacogn J. 2020; 12(1): 20–4. doi: https://doi.org/10.5530/pj.2020.12.4
Moghadamtousi SZ, Fadaeinasab M, Nikzad S, Mohan G, Ali HM, Kadir HA. Annona muricata (Annonaceae): A review of its traditional uses, isolated acetogenins and biological activities. Int J Mol Sci. 2015; 16(7): 15625–58. doi: https://doi.org/10.3390/ijms160715625
Abdul Wahab SM, Jantan I, Haque MA, Arshad L. Exploring the leaves of Annona muricata L. as a source of potential anti-inflammatory and anticancer agents. Front Pharmacol. 2018; 9: 661. doi: https://doi.org/10.3389/fphar.2018.00661
Thouri A, Chahdoura H, El Arem A, Omri Hichri A, Ben Hassin R, Achour L. Effect of solvents extraction on phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti). BMC Complement Altern Med. 2017; 17(1): 248. doi: https://doi.org/10.1186/s12906-017-1751-y
Syafitri NE, Bintang M, Falah S. Kandungan fitokimia, total fenol, dan total flavonoid ekstrak buah harendong (Melastoma affine D. Don). Curr Biochem. 2014; 1(3): 105–15. web: https://journal.ipb.ac.id/index.php/cbj/article/view/17419
Ribeiro IP, Rodrigues JM, Mascarenhas A, Kosyakova N, Caramelo F, Liehr T, Melo JB, Carreira IM. Cytogenetic, genomic, and epigenetic characterization of the HSC-3 tongue cell line with lymph node metastasis. J Oral Sci. 2018; 60(1): 70–81. doi: https://doi.org/10.2334/josnusd.16-0811
Dojindo Laboratories. Measuring cell viability/cytotoxicity: Cell counting kit-8. Cell viability and cytotoxicity assay: Protocol. Dojindo Molecular Technologies, Inc.; 2020. p. 4–15. web: https://dojindo.com/protocol/
Cai L, Qin X, Xu Z, Song Y, Jiang H, Wu Y, Ruan H, Chen J. Comparison of cytotoxicity evaluation of anticancer drugs between real-time cell analysis and CCK-8 method. ACS omega. 2019; 4(7): 12036–42. doi: https://doi.org/10.1021/acsomega.9b01142
Damasuri AR, Sholikhah EN, Mustofa. Cytotoxicity of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) on HeLa cell line. Indones J Pharmacol Ther. 2020; 1(2): 54–9. doi: https://doi.org/10.22146/ijpther.606
Gavamukulya Y, Wamunyokoli F, El-Shemy HA. Annona muricata: Is the natural therapy to most disease conditions including cancer growing in our backyard? A systematic review of its research history and future prospects. Asian Pac J Trop Med. 2017; 10(9): 835–48. doi: https://doi.org/10.1016/j.apjtm.2017.08.009
Dasari S, Tchounwou PB. Cisplatin in cancer therapy: molecular mechanisms of action. Eur J Pharmacol. 2014; 740: 364–78. doi: https://doi.org/10.1016/j.ejphar.2014.07.025
Pramadya PP, Hendryana MA. Efek ekstrak metanol daun sirsak (Annona muricata) dalam menghambat pertumbuhan bakteri Salmonella typhi secara in vitro. J Med Udaya. 2020; 9(9): 65–9. web: https://ojs.unud.ac.id/index.php/eum/article/view/65978
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