The effect of 5% Curcuma xanthorrhiza extract gel on diabetic rat socket: A fibroblast analysis
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Background: Predominant advanced glycation end products (AGEs) in diabetes mellitus (DM) patients lead to increased reactive oxygen species, causing oxidative stress and cell apoptosis, which hinders wound healing. Curcuma xanthorrhiza contains active compounds such as curcumin, flavonoids, and saponins, which can increase fibroblast cell production, the primary indicator of wound healing, and serve as AGE inhibitors. Purpose: This study aimed to determine the effect of 5% C. xanthorrhiza extract gel on the number of fibroblasts in diabetic Wistar rats during the healing of tooth extraction over a specific duration. Methods: The left mandibular incisor was extracted after using alloxan to induce diabetes in the rats. The socket was then treated with different compounds, including 5% C. xanthorrhiza extract gel, placebo CMC-Na, or Gengigel. A histopathological examination of the socket was conducted to assess the presence of fibroblasts on days one, three, and five after treatment. Results: The number of fibroblasts in the sockets treated with 5% C. xanthorrhiza extract gel was lower than in the Gengigel group but higher than in the placebo group (p < 0.05). Conclusion: 5% C. xanthorrhiza extract gel can increase the number of fibroblasts, thereby potentially accelerating wound healing in DM.
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Majeed W, Khaliq T, Aslam B, Khan JA. Polyherbal formulation prevents hyperglycemia by modulating the biochemical parameters and upregulating the insulin signaling cascade in alloxan induced hyperglycemic rats. Pak Vet J. 2018; 38(2): 121–6. doi: https://doi.org/10.29261/pakvetj/2018.035
Lede MJ, Hariyanto T, Ardiyani VM. Pengaruh kadar gula darah terhadap penyembuhan luka diabetes mellitus di Puskesmas Dinoyo Malang. Nurs News (Meriden). 2018; 3(1): 539–47. web: https://publikasi.unitri.ac.id/index.php/fikes/article/view/826
Kementerian Kesehatan RI. Riskesdas 2018. Laporan Nasional Riskesdas 2018. Jakarta: Balitbang Kemenkes RI; 2018. p. 127–30. web: http://repository.bkpk.kemkes.go.id/3514/
Milita F, Handayani S, Setiaji B. Kejadian diabetes mellitus tipe II pada lanjut usia di Indonesia (Analisis Riskesdas 2018). J Kedokt dan Kesehat. 2021; 17(1): 9–20. doi: https://doi.org/10.24853/jkk.17.1.9-20
Luthfi M, Kusumaningsih T, Sosiawan A, Sabrina H. Anti-inflammatory effect of okra (Abelmoschus esculentus) fruit extract during wound healing process after tooth extraction of diabetic Wistar rat. J Int Dent Med Res. 2020; 13(2): 497–502. web: http://www.jidmr.com/journal/wp-content/uploads/2020/06/18-D20_1030_Muhammad_Luthfi_Indonesia.pdf
Soesilawati P, Rachmat EA, Arundina I, Naomi N. The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction. Dent J. 2021; 54(3): 124–7. doi: https://doi.org/10.20473/j.djmkg.v54.i3.p124-127
Dai J, Chen H, Chai Y. Advanced glycation end products (AGEs) induce apoptosis of fibroblasts by activation of NLRP3 inflammasome via reactive oxygen species (ROS) signaling pathway. Med Sci Monit. 2019; 25: 7499–508. doi: https://doi.org/10.12659/MSM.915806
Kurniawati A, Kristanti YD, Rahmat NA, Rahayu YC, Cholid Z, Sosiawan A. The role of purple leaves extract (Graptophyllum Pictum (L.) Griff) on the number of fibroblasts and blood vessels in the socket after tooth extraction. Dent J. 2024; 57(1): 56–61. doi: https://doi.org/10.20473/j.djmkg.v57.i1.p56-61
Zulfa IM. Studi potensi interaksi obat-obat pada peresepan penyakit gigi di salah satu Apotek di Surabaya. J Pharm Sci. 2018; 3(1): 21–4. doi: https://doi.org/10.53342/pharmasci.v3i1.70
Sarkarat F, Modarresi A, Riyahi A, Mortazavi P, Tabandeh F, Rakhshan V. Efficacy of hyaluronic acid, absorbable collagen sponge, and their combination in minimizing bisphosphonate-related osteonecrosis of the jaws (BRONJ) after dental extraction: a preliminary animal histomorphometric study. Maxillofac Plast Reconstr Surg. 2022; 44(1): 8. doi: https://doi.org/10.1186/s40902-022-00337-7
Marouf I, Rejab A. Effects of local application of hyaluronic acid on postoperative sequelae after surgical removal of impacted lower third molars. Al-Rafidain Dent J. 2020; 20(2): 154–64. doi: https://doi.org/10.33899/rden.2020.126803.1026
Marliani L, Sukmawati IK, Juanda D, Anjani E, Anggraeni I. Penapisan fitokimia, kadar kurkuminoid dan aktivitas antibakteri temu hitam (Curcuma aeruginosa (Christm) Roscoe.), temu putih (Curcuma zedoaria Roxb.) dan temulawak (Curcuma xanthorrhiza Roxb.). Herb-Medicine J. 2021; 4(1): 57–64. doi: https://doi.org/10.30595/hmj.v4i1.9092
Syamsudin RAMR, Perdana F, Mutiaz FS, Galuh V, Rina APA, Cahyani ND, Aprilya S, Yanti R, Khendri F. Temulawak plant (Curcuma xanthorrhiza Roxb) as a traditional medicine. J Ilm Farm Bahari. 2019; 10(1): 51–65. doi: https://doi.org/10.52434/jfb.v10i1.648
Irfan K, Ayyasi IA, Ilham NHR, Rebriarina H. Effect of Curcuma xanthorrhiza gel on methicillin-resistant Staphylococcus aureus-infected second-degree burn wound in rats. Nat Prod Sci. 2021; 27(1): 1–9. doi: https://doi.org/10.20307/nps.2021.27.1.1
Rahmat E, Lee J, Kang Y. Javanese turmeric (Curcuma xanthorrhiza Roxb.): Ethnobotany, phytochemistry, biotechnology, and pharmacological activities. Evid Based Complement Alternat Med. 2021; 2021: 9960813. doi: https://doi.org/10.1155/2021/9960813
Alizadeh M, Kheirouri S. Curcumin against advanced glycation end products (AGEs) and AGEs-induced detrimental agents. Crit Rev Food Sci Nutr. 2019; 59(7): 1169–77. doi: https://doi.org/10.1080/10408398.2017.1396200
Panggabean RT, Sudjarwo SA, Ma'ruf A, Widiyatno TV, Yudaniayanti IS, Kurnijasanti R, Khairullah AR. Efficacy of Sauropus androgynous leaves extract gel on burn wound healing in albino rats. J Med Vet. 2023; 6(3): 107–15. doi: https://doi.org/10.20473/jmv.vol6.iss3.2023.107-115
Hertian R, Muhaimin, Sani K F. Uji efektivitas ekstrak daun ekor naga (Rhaphidohora pinnata (L.f) Schott) terhadap penyembuhan luka sayat pada mencit putih jantan. Indones J Pharma Sci. 2021; 1(1): 5–24. web: https://online-journal.unja.ac.id/IJPS/article/view/13593
Sabuding MiA, Sujono TA. Uji aktivitas antidiabetes ekstrak etanol bunga kertas (Bougainvillea spectabilis Willd.) pada tikus Wistar yang diinduksi aloksan. Universitas Muhammadiyah Surakarta; 2018. p. 1–16. web: https://eprints.ums.ac.id/59447/
Farida Y, Rahmat D, Amanda AW. Anti-inflammation activity test of nanoparticles ethanol extract of temulawak rhizome (Curcuma xanthorrhiza Roxb.) with protein denaturation inhibition method. J Ilmu Kefarmasian Indones. 2018; 16(2): 225–30. doi: https://doi.org/10.35814/jifi.v16i2.569
Megawati A, Yuliana S. Uji efek ekstrak etanol rimpang temulawak (Curcuma Xanthorrhiza Roxb.) terhadap penurunan kadar asam urat tikus Wistar yang diinduksi potasium oksonat secara in vivo. Cendekia J Pharm. 2019; 3(2): 85–95. doi: https://doi.org/10.31596/cjp.v3i2.57
Muharrami LK, Munawaroh F, Ersam T, Santoso M. Phytochemical screening of ethanolic extract: A preliminary test on five medicinal plants on Bangkalan. J Pena Sains. 2020; 7(2): 96–102. doi: https://doi.org/10.21107/jps.v7i2.8722
Direktorat Jenderal Kefarmasian dan Alat Kesehatan. Farmakope herbal Indonesia. 2nd ed. Jakarta: Kementerian Kesehatan Republik Indonesia; 2017. p. 499–501. web: https://repository.kemkes.go.id/book/392
Suharsanti R, Astutiningsih C, Susilowati ND, Tinggi S, Farmasi I, Semarang YP. Kadar kurkumin ekstrak rimpang kunyit (Curcuma domestica) secara KLT densitometri dengan perbedaan metode ekstraksi. J Wiyata. 2020; 7(2): 86–93. web: https://ojs.iik.ac.id/index.php/wiyata/article/view/387
Kusuma TM, Azalea M, Dianita P, Syifa N. Pengaruh variasi jenis dan konsentrasi gelling agent terhadap sifat fisik gel hidrokortison. J Farm Sains dan Prakt. 2018; 4(1): 44–9. doi: https://doi.org/10.31603/pharmacy.v4i1.2589
Zubaidah N, Luthfi M, Setyari W, Palupi R, Ummah NI, Maheswari AL, Nurdianto AR, Razak FA. Regenerative alveolar bone in dental sockets of diabetic wistar rats post tooth extraction. Bali Med J. 2022; 11(3): 1699–705. doi: https://doi.org/10.15562/bmj.v11i3.3874
Siregar KS, Ummami R. Teknik eutanasi dan nekropsi tikus (Rattus norvegicus) di National Laboratory Animal Center (NLAC), Mahidol University, Thailand periode 20 November-2 Desember 2017. Gadjah Mada University; 2018. p. 8–15. web: https://etd.repository.ugm.ac.id/penelitian/detail/157662
Ernawati DS, Nugraha AP, Narmada IB, Ardani IGAW, Hamid T, Triwardhani A, Winoto ER, Alida A, Susanto H, Ramadhani NF, Brahmanta A, Nugraha AP, Ihsan IS, Riawan W, Saskianti T, Noor TNE binti TA. The number of osteoblast and osteoclast during orthodontic tooth movement after preconditioned gingiva mesenchymal stem cell allogeneic transplantation in vivo. J Int Dent Med Res. 2022; 15(3): 1069–77. web: http://www.jidmr.com/journal/wp-content/uploads/2022/09/18-D22_1865_Alexander_Patera_Nugraha_Indonesia.pdf
Lunardhi LC, Kresnoadi U, Agustono B. The effect of a combination of propolis extract and bovine bone graft on the quantity of fibroblasts, osteoblasts and osteoclasts in tooth extraction sockets. Dent J. 2019; 52(3): 126–32. doi: https://doi.org/10.20473/j.djmkg.v52.i3.p126-132
Suwardi OA, Ranggaini MD. Aktivitas antioksidan ekstrak etanol rimpang Curcuma xanthorrhiza roxb. dan asam askorbat (Dengan metode DPPH, ABTS, dan NO). J Kedokt Gigi Terpadu. 2022; 4(1): 83–8. web: https://e-journal.trisakti.ac.id/index.php/jkgt/article/view/14475
Akrianto A. Pengaruh sterilisasi terhadap kadar alkaline phospatse (alp) pada tikus putih (Rattus Norvegicus). Hasanuddin University; 2021. p. 15–26. web: http://repository.unhas.ac.id/id/eprint/5563/
Hartono D. Hubungan self care dengan komplikasi diabetes mellitus pada pasien diabetes mellitus tipe II di Poli Penyakit Dalam RSUD Dokter Mohamad Saleh Kota Probolinggo. J Nurs Care Biomol. 2019; 4(2): 111–8. web: https://jnc.stikesmaharani.ac.id/index.php/JNC/article/view/144/
Kawano Y, Patrulea V, Sublet E, Borchard G, Iyoda T, Kageyama R, Morita A, Seino S, Yoshida H, Jordan O, Hanawa T. Wound healing promotion by hyaluronic acid: Effect of molecular weight on gene expression and in vivo wound closure. Pharmaceuticals. 2021; 14(4): 301. doi: https://doi.org/10.3390/ph14040301
Mardiyantoro F, Prasetyaningrum N, Hidayat LH, Khasanah U, Roring VC, Muizun A, Kardikadewi VAW. Pangasius djambal gelatin increases osteoprotegerin, decreases RANK and RANKL after tooth extraction in vivo. Dent J. 2023; 56(4): 238–42. doi: https://doi.org/10.20473/j.djmkg.v56.i4.p238-242
Kristianto H, Khotimah H, Sholeha RA, Mardianto EW, Sani HC, Paratiwi HA. The effects of Javanese turmeric (Curcuma xanthorriza Roxb) on fibroblasts, granulation, blood vessel density, and contraction in wound healing of STZ-induced diabetic rats. Kuwait J Sci. 2022; 50(1A): 1–14. doi: https://doi.org/10.48129/kjs.15261
Rujirachotiwat A, Suttamanatwong S. Curcumin upregulates transforming growth factor-β1, its receptors, and vascular endothelial growth factor expressions in an in vitro human gingival fibroblast wound healing model. BMC Oral Health. 2021; 21(1): 535. doi: https://doi.org/10.1186/s12903-021-01890-9
Wulandari P, Hutagalung M, Perdanakusuma D. Deteksi kadar transforming growth factor (Tgf-Î’) pada luka akut. J Rekonstruksi dan Estet. 2021; 6(1): 1–3. doi: https://doi.org/10.20473/jre.v6i1.28225
Barchitta M, Maugeri A, Favara G, Magnano San Lio R, Evola G, Agodi A, Basile G. Nutrition and wound healing: An overview focusing on the beneficial effects of curcumin. Int J Mol Sci. 2019; 20(5): 1119. doi: https://doi.org/10.3390/ijms20051119
Emilda E. Efek senyawa bioaktif kayu manis (Cinnamomum burmanii Nees Ex.Bl.) terhadap diabetes melitus: kajian pustaka. J Fitofarmaka Indones. 2018; 5(1): 246–52. doi: https://doi.org/10.33096/jffi.v5i1.316
Wang H, Xu Z, Zhao M, Liu G, Wu J. Advances of hydrogel dressings in diabetic wounds. Biomater Sci. 2021; 9(5): 1530–46. doi: https://doi.org/10.1039/D0BM01747G
Amfotis ML, Suarni NMR, Arpiwi NL. Wound healing of cuts in the skin of white rat (Rattus norvegicus) is given kirinyuh (Chromolaena odorata) leaf extract. Metamorf J Biol Sci. 2022; 9(1): 139–51. doi: https://doi.org/10.24843/metamorfosa.2022.v09.i01.p14
Luhurningtyas FP, Susilo J, Yuswantina R, Widhihastuti E, Ardiyansah FW. Aktivitas imunomodulator dan kandungan fenol ekstrak terpurifikasi rimpang jahe merah. Indones J Pharm Nat Prod. 2021; 4(1): 51–9. doi: https://doi.org/10.35473/ijpnp.v4i1.974
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