Pemberian Topikal Ekstrak Daun Pepaya (Carica papaya) pada Hewan Coba Mencit (Mus musculus) Bunting Meningkatkan Kepadatan Kolagen Jaringan Vagina
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Tujuan: Menganalisis pengaruh pemberian gel ekstrak air daun pepaya terhadap kepadatan kolagen jaringan vagina pasca melahirkan pada hewan coba mencit saat bunting.
Bahan dan Metode: Desain penelitian randomized post test only control group,dilakukan di Departemen Embriologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga, pada bulan April-Juni 2015. Terdapat empat kelompok, masing-masing kelompok terdiri dari 8 mencit bunting. Kelompok kontrol (tanpa perlakuan) dan kelompok perlakuan berupa pemberian topikal ekstrak daun pepaya konsentrasi 5%, 10% dan 15%, 1x/hari pervaginam, sejak usia kebuntingan hari ke-12 sampai melahirkan. Lama perlakuan 6-8 hari. Setelah melahirkan, mencit dikorbankan dan diperiksa kepadatan kolagen dengan pewarnaan HE. Uji statistik yang dipakai uji parametrik dan nonparametrik disertai uji komparasi ganda
Hasil: Kepadatan kolagen berdasarkan skor didapatkan nilai median pada kelompok I = 3,5 (3-4); kelompok II = 4 (4-4); kelompok III = 3 (3-4); kelompok IV = 4 (3-4) dengan uji Kruskal-Wallis (p=0,036) menunjukkan perbedaan signifikan, dilanjutkan uji beda tiap kelompok Mann-Whitney, hasilnya tidak didapatkan perbedaan signifikan antara kelompok kontrol dengan masing-masing kelompok perlakuan. Kepadatan kolagen berdasarkan persentase didapatkan nilai rerata pada kelompok I = 90,64%±3,35; kelompok II = 96,19%±1,34; kelompok III = 87,85%±6,69 dan kelompok IV = 92,08%±4,84. Uji Anova menunjukkan perbedaan signifikan (p=0,032), dilanjutkan uji beda LSD menunjukkan perbedaan bermakna antara kelompok I dan II (p=0,045)
Simpulan: pemberian topikal ekstrak daun pepaya konsentrasi 5% pervaginam saat masa kebuntingan mencit meningkatan kepadatan kolagen jaringan vagina pasca melahirkan.
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