The correlation between the use of personal protective equipment and level wild-type p53 of dental technicians in Surabaya

Puspa Dila Rohmaniar, Titiek Berniyanti, Retno Pudji Rahayu

DOI: http://dx.doi.org/10.20473/j.djmkg.v50.i1.p19-22
Abstract viewed = 183 times | PDF downloaded = 85 times

Abstract


Background: Exposure of metals among dental technicians that come from the working environment can lead to the formation reactive oxygen species (ROS). ROS can cause mutations in the p53 gene (p53). The mutation is transversion mutation GuanineThymine. p53 mutations can lead to low expression of the wild-type p53 protein (p53). Wild-type p53 involved in many biological processes such as regulation of genes involved in cell cycle, cell growth after DNA damage, and apoptosis. However, exposure to metals among dental technicians can be prevented through the use of personal protective equipment (PPE) during work. Purpose: The purpose of this study was to analyze the correlation between the use of personal protective equipment to wild-type p53 protein levels among dental technicians in Surabaya. Method: This study was observational analytic with cross sectional approach. 40 samples were taken by random sampling. Data were retrieved through interviews and observations. Wild-type p53 was analyzed from saliva with indirect ELISA method. Analysis of data used Kolmogorov Smirnov normality test and a Pearson correlation test. Value significance was p<0.05 (95% confidence level). Result: There was a significant association between the use of personal protective equipment with wild-type p53 levels with p=0.002 Conclusion: The use PPE properly is positively correlated with the wild-type p53 protein levels of dental technicians in Surabaya.


Keywords


personal protective equipment; dental technician; P53 wild

Full Text:

PDF

References


Anusavice KJ, Shen C, Rawls HR. Phillips’ science of dental materials. 12th ed. St. Louis: Saunders; 2013. p. 8.

Al-Hourani Z. Chromium and cobalt levels among dental technicians in the northern jordan. European Scientific Journal 2013; 9(21): 130.

Hariyani N, Berniyanti T, Setyowati D. Effects of occupational environmental controls on the level of Co, Ni and Cr among dental technicians. International Journal of Environmental Science and Development 2015; 6(9): 1-4.

Ergün D, Ergün R, Ozdemir C, Oziş TN, Yilmaz H, Akkurt I. Pneumoconiosis and respiratory problems in dental laboratory technicians: analysis of 893 dental technicians. Int J Occup Med Environ Health 2014; 27(5): 785-96.

Petroviü D, Kruniü N, Kostiü M. Risk factors and preventive measures for occupational diseases in dental technicians .Vojnosanit Pregl 2013; 70(10): 959–63.

Valko M, Morris H, Cronin MT. Metals, toxicity and oxidative stress. Curr Med Chem 2005; 12(10): p. 1161-208.

Lu H, Shi X, Costa M, Huang C. Carcinogenic effect of nickel compounds. Mol Cell Biochem 2005; 279(1-2): 45-67

Hassan NM, Tada M, Hamada J. Presence of dominant negative mutation of TP53 is a risk of early recurrence of oral cancer. Cancer Lett 2008; 270(1): 108-19.

Munir D, Lutan R, Hasibuan M, Henny F. Ekspresi protein p53 mutan pada karsinoma nasofaring. Majalah Kedokteran Nusantara 2007; 40(3): 168.

Kumar V, Robbins, Neoplasia LS. In: Robbins & Cotran pathologic basis of disease. 8th ed. Philadelphia: Saunders Elsevier; 2010. p. 269-342.

Istindiah HN, Auerkari EI. Penggunaan saliva untuk mendeteksi kanker. Jurnal Kedokteran Gigi Universitas Indonesia 2003; vol 10 (Edisi Khusus): 279-81.

Hozzeini FA, Dizgah IM, Zarandi NS. Unstimulated salivary p53 in patients with oral lichen planus and squamous cell carcinoma. Acta Medica Iranica 2015; 53(7): 440-43.

Lee JJ, Kuo MY, Cheng SJ, Chiang CP, Jeng JH, Chang HH, Kuo YS, Lan WH, Kok SH. Higher expressions of p53 and proliferating cell nuclear antigen (PCNA) in atrophic oral lichen planus and patients with areca quid chewing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99(4): p. 471-8.

Engelen L, de Wijk RA, Prinz JF, van der Bilt A, Bosman F. The relation between saliva flow after different stimulations and the perception of flavor and texture attributes in custard desserts. Physiol Behav 2003; 78(1): 165-9.

Costa M. Molecular mechanism of nickel carcinogenesis. Biol Chem 2002; 383(6): p 69-75

Risdayanti A. Perilaku pemakaian alat pelindung diri pada teknisi gigi terhadap paparan bahan kimia di laboratorium gigi surabaya. Skripsi. Surabaya: Fakultas Kedokteran Gigi Universitas Airlangga; 2010.

Stephen L, Harris G, Xianlin. Metal- induced oxidative stress and signal transduction. Free Radical Biology and medicine 2004; 37(12): 1921-42.

Sudjarwo .8-hidroksi-deoksiguanosin sebagai salah satu indikator infertilitas pria. Berk. Penel. Hayati; 2004: vol 10. p. 43–47

Streckfus C, Bigler L, Tucci, Thigpen JT. A preliminary study of cal5-3, c-erbb-2, epidermal growth factor receptor, cathepsin-d, and p53 in saliva among women with breast carcinoma. Cancer Investigation 2000; 18(2): 101-9.

Kundie, FAM. Mohamed, SH. Issaid, MA. Omran, A. Evaluation of dental technicians awareness of health and safety rule in dental laboratories at some cities in libya. International Journal of Engineering; 2010: 7(2). P. 126.

Phalen, RN. Le, T. Wong, WK. Changes in Chemical Permeation of Disposable Latex, Nitrile and Vinyl Gloves Exposed to Simulated Movement. J Occup Environ Hyg 2014; 11(11): p. 716–721

Sunarto. Keselamatan dan Kesehatan Kerja Laboratorium Kimia. Yogyakarta: FMIPA UNY; 2008. p. 3.

Tran, TAD. Arnold, M. Schacher, L. Adolphe, DC. Reys, G. Development of Personal Protection Equipment for Medical Staff: Case of Dental Surgeon. AUTEX Research Journal 2015; 15(4): pp. 280-7.

Yurdasal, B. Bozkurt, N. Bozkurt, AI. Yilmaz, O. The evaluation of the dust-related occupational respiratory disorders of dental laboratory technicians working in Denizli Province. Annals of Thoracic Medicine; 2015: 10(4). pp. 249-255.

Susanto DA. Pnumokoniosis. J. Indon Med Assoc. 2011; 61(12): 503-50.

Wirasuta IMG, Niruri R. Buku ajar toksikologi umum. Denpasar: Farmakologi Universitas Udayana; 2010. p. 12.

Djojodibroto D. Respirologi. Jakarta: Penerbit Buku Kedokteran EGC; 2007. p. 11-12.

Todd Davidson, Qunwei Zhang, Lung Chi Chen, Weichen Su, and Max Costa. The Involvement of Hypoxia-inducible Transcription Factor-1-dependent in Nickel Carcinogenesis. Cancer Research 2003; vol 63: p 3524–3530.

Obacz J, Pastorekova S, Vojtesek, and Hrstka R. Cross-talk between HIF and p53 as mediators of molecular responses to physiological and genotoxic stresses. Mol Cancer 2013; 12(93): p 2-7


Refbacks

  • There are currently no refbacks.