Acceleration of post-tooth extraction socket healing after continuous aerobic and anaerobic physical exercise in Wistar rats (Rattus norvegicus)

Aqsa Sjuhada Oki, Moch Febi Alviansyah, Christian Khoswanto, Retno Pudji Rahayu, Muhammad Luthfi

Abstract views = 1064 times | downloads = 555 times


Background: Physical exercise has been proven to accelerate wound healing. Physical training itself consists of aerobic (continuous training) and anaerobic (interval training) exercise. The effectiveness of continuous physical exercise on post-tooth extraction wound healing is the focus of this study. Purpose: This study aims to investigate the differences in post-tooth extraction wound healing in Wistar rats (Rattus norvegicus) after aerobic and anaerobic exercise based on the number of fibroblasts and neovascularisation. Methods: Wistar rats were divided into three groups: the control group (K1); K2 undertook continuous aerobic exercise, swimming at 50% maximum swimming capacity (MSC) with an additional 3% bodyweight load; K3 undertook anaerobic continuous exercise, swimming at 65% MSC with a 6% load. The rats swam three times per week for six weeks. The number of fibroblasts and neovascularisation were examined three days after tooth extraction. Data was analysed using the one-way analysis of variance (ANOVA) and Least Significant Difference (LSD) tests (p<0.05). Results: There was a significant difference in the number of fibroblasts between the K2 and K3 groups. There was no significant difference between K2 and K3 in the amount of neovascularisation. Conclusion: There were differences in the number of fibroblasts but not neovascularisation after tooth extraction in Wistar rats given aerobic and anaerobic continuous training.


continuous aerobic physical exercise; continuous anaerobic physical exercise; fibroblasts; neovascularisation; post-tooth extraction wound healing

Full Text:



Fakhrurrazi, Hakim RF, Ulfa L. The differences blood glucose levels at random before and after tooth extraction of the patients at dental installation RSUDZA Banda Aceh. Cakradonya Dent J. 2017; 9(2): 96–100.

Preetha S. An overview of dry socket and its management. IOSR J Dent Med Sci. 2014; 13(5): 32–5.

Wright J, Paauw DS. Complications of antibiotic therapy. Med Clin North Am. 2013; 97(4): 667–79.

Blumenthal KG, Peter JG, Trubiano JA, Phillips EJ. Antibiotic allergy. Lancet. 2019; 393(10167): 183–98.

Prasetyoputri A, Jarrad AM, Cooper MA, Blaskovich MAT. The eagle effect and antibiotic-induced persistence: Two sides of the same coin? Trends Microbiol. 2019; 27(4): 339–54.

Harun L. Perbandingan kadar interleukin-6 dan jumlah limfosit setelah latihan aerobik ringan dan sedang pada remaja. Heal J. 2018; 1(2): 64–8.

Keylock KT, Vieira VJ, Wallig MA, DiPietro LA, Schrementi M, Woods JA. Exercise accelerates cutaneous wound healing and decreases wound inflammation in aged mice. Am J Physiol - Regul Integr Comp Physiol. 2008; 294(1): R179–84.

Pence BD, Woods JA. Exercise, obesity, and cutaneous wound healing: Evidence from rodent and human studies. Adv Wound Care. 2014; 3(1): 71–9.

Oki AS, Bimarahmanda ME, Rahardjo MB. Increased number of fibroblasts and neovascularization after tooth extraction in wistar rats with moderate-intensity continuous exercise. J Int Dent Med Res. 2018; 11(3): 840–5.

Oki AS, Amalia N, Tantiana. Wound healing acceleration in inflammation phase of post-tooth extraction after aerobic and anaerobic exercise. Sci Sport. 2020; 35(3): 168.e1-168.e6.

Gonzalez ACDO, Andrade ZDA, Costa TF, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016; 91(5): 614–20.

Olczyk P, Mencner Ł, Komosinska-Vassev K. The role of the extracellular matrix components in cutaneous wound healing. Biomed Res Int. 2014; 2014: 747584.

Ercan E. The effect of platelet-rich fibrin and titanium prepared platelet-rich fibrin on early soft tissue healing of extraction sites. Cumhur Dent J. 2018; 21(4): 304–10.

Way KL, Sultana RN, Sabag A, Baker MK, Johnson NA. The effect of high Intensity interval training versus moderate intensity continuous training on arterial stiffness and 24 h blood pressure responses: A systematic review and meta-analysis. J Sci Med Sport. 2019; 22(4): 385–91.

Khoswanto C. A new technique for research on wound healing through extraction of mandibular lower incisors in Wistar rats. Eur J Dent. 2019; 13(2): 235–7.

Oki AS, Farhana N, Yuliati. The effect of aerobic and anaerobic interval exercise on the proliferation phase of wound healing in tooth extraction of Rattus novergicus. Acta Med Philipp. 2019; 53(5): 417–22.

Setyadewi W, Oki AS, Sunariani J. Moderate intensity physical exercise effect on PMN and macrophage expression in Rattus norvegicus post tooth extraction. J Int Dent Med Res. 2017; 10(2): 364–7.

Goh J, Ladiges WC. Exercise enhances wound healing and prevents cancer progression during aging by targeting macrophage polarity. Mech Ageing Dev. 2014; 139(1): 41–8.

Rashidi M, Salehian O, Vaezi G. The effect of high intensity anaerobic training on the blood lactate levels after active recovery. Eur J Exp Biol. 2013; 3(6): 346–50.

Irmawati A, Giffari FZ, Oki AS. The effect of moderate exercise on vascular endothelial growth factor expression during tooth socket wound healing after tooth extraction. J Postgrad Med Inst. 2018; 32(1): 19–23.

Ding J, Tredget EE. The role of chemokines in fibrotic wound healing. Adv Wound Care. 2015; 4(11): 673–86.

Shi M, Wang X, Yamanaka T, Ogita F, Nakatani K, Takeuchi T. Effects of anaerobic exercise and aerobic exercise on biomarkers of oxidative stress. Environ Health Prev Med. 2007; 12(5): 202–8.

Tatullo M. The regenerative dentistry: Current approaches and future insights. Cumhur Dent J. 2020; 23(1): 1–3.

Flora R. Pengaruh latihan fisik anaerobik terhadap kadar laktat plasma dan kadar laktat jaringan otot jantung tikus Wistar. Biomed J Indones. 2015; 1(1): 40–2.


  • There are currently no refbacks.

View My Stats