Produksi Enzim Fibrinolitik Tempe oleh Rhizopus oryzae FNCC 6078
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ackground Rhizopus oryzae FNCC 6078 had been evaluated producing fibrinolytic enzyme under solid state fermentation. Soybean had been used to produce fibrinolytic enzyme through fermentation in tempeh. The main purpose of this study was to reveal optimum condition for fermentation. The parameters of the condition were inoculum volume, incubation period and temperature. Optimum condition was defined by maximum fibrinolytic activity. Methode Fibrinolytic activity was measured using spectrophotometry at 274 nm. Result optimum condition for producing fibrinolytic enzyme was 1,5 mL volume of inoculum of Rhizopus oryzae suspension in 25%T, 42 hours for incubation period and 35oC temperature incubation.
Arunachalam, C., and Aiswarya, M.,2011. Microbial fibrinolytic enzyme – a deity for thrombolysis. Advanced Biotech, Vol. 10, Issue. 9, pp. 8 – 11.
Bakta, I.M., 2007. Thrombosis dan usia lanjut. J. Peny. Dalam, Vol. 8, No.2, hal. 148-160.
Han, B.Z., and Nout, M.J.R., 2001, Effect of temperature, watter activity, and gas atmosphere on mycelia growth of tempe fungi Rhizopus microsporus var. microsporus and R. microsporus var. oligosporus. World Journal of Microbiology and Biotechnology, 16, pp. 853- 858.
Kotb, E., 2012. Fibrinolytic Bacterial Enzymes with Thrombolytic Activity. Heidelberg: Springer.
Liu, J.G., Xing, J.M., Chang, T.S., Liu, H.Z., 2006. Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio. Bioprocess Biosyst Eng, pp. 267-273
Rauf, A., Irfan, M., Nadeem, M., Ahmed, I., Iqbal, H.M.N., 2010. Optimization of growth conditions from Rhizopus oligosporus through solid state fermentation of sunflower meal. International Journal of Agricultural and Biological Sciences, 1:1, pp. 40-43
Sher, M.G., Nadeem, M., Syed, Q., Abass, S., and Hassan, A., 2011. Study on protease from barley tempeh and in vitro protein digestibility. Jordan Journal of Biological Sciences, vol. 4, no. 4, pp. 257-264.
Sugimoto, S., Fuji, T., Morimiya, T., Johdo, O., and Nakamura, T., 2007. The Fibrinolytic Activity of a Novel Protease Derived from a Tempeh Producing Fungus Fusarium sp. BLB. Biosci, Botechnol, Biochem, 71, 70153-1-6.
Suri, W.L., Syukur, S., and Jamsari, 2013. Optimization of protease activity from Lactic Acid Bacteria (LAB) Pediococcus pentosaceus isolated from soursop fermentation (Annona muricata L.). Jurnal Kimia Unand, vol.2, no.1, pp. 18-25.
Tunga, R., Banerjee, R., Bhattacharyya, B.C., 1998. Optimizing some factors affecting protease production under solid state fermentation. Bioprocess Enginering, 19, pp. 187-190.
Yang, H. J., Park, S., Pak, V., Chung, K. R., Kwon, D. Y., 2011. Fermented soybean products and their bioactive compounds. In: H. El-Shemy. Soybean and Health, Egypt: InTech, pp. 22- 53.
Yoon, Seon-Joo, Yu, M., Sim, G., Kwon, S., Hwang, J., Shin, J., Yeo, I., and Pyun, Y., 2002. Screening and characterization of microorganism with fibrinolytic activity from fermented foods. J. Microbiol Biotechnol, 12(4), pp. 649-656.
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