In Vitro Antifungal Susceptibility Testing of Tea Tree Oil (TTO) 5% Compared with Nystatin against Candida sp. as Important Agent of Oral Candidiasis in HIV/AIDS Patients

tea tree oil Melaleuca alternifolia nystatin antifungal susceptibility testing oral candidiasis HIV/AIDS

Authors

  • Eva Lydiawati Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga /Dr. Soetomo General Academic Teaching Hospital, Surabaya
  • Muhammad Yulianto Listiawan Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga /Dr. Soetomo General Academic Teaching Hospital, Surabaya
  • Dwi Murtiastutik Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga /Dr. Soetomo General Academic Teaching Hospital, Surabaya
  • Rahmadewi Rahmadewi Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga /Dr. Soetomo General Academic Teaching Hospital, Surabaya
  • Cita Rosita Sigit Prakoeswa Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga /Dr. Soetomo General Academic Teaching Hospital, Surabaya
  • Christina Avanti Faculty of Pharmacy, Universitas Surabaya, Surabaya
  • Endang Wahyu Fitriani Faculty of Pharmacy, Universitas Surabaya, Surabaya
  • Erwin Astha Triyono Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga/Dr. Soetomo General Academic Teaching Hospital, Surabaya
  • Linda Astari Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga /Dr. Soetomo General Academic Teaching Hospital, Surabaya
  • Iskandar Zulkarnain
    zuljazid@yahoo.com
    Department of Dermatology and Venereology, Faculty of Medicine, Universitas Airlangga /Dr. Soetomo General Academic Teaching Hospital, Surabaya
November 30, 2020

Downloads

Background: Oral candidiasis is the most common clinical manifestation in a person infected with HIV which has been linked with the severity of the disease. Candida albicans responsible for most oral candidiasis. The increased resistance to the available antifungal drugs can reduce the efficacy of therapy. Therefore, it will be crucial to discover novel antifungal agents. One of them is tea tree oil (TTO) or Melaleuca alternifolia which has a potent antifungal effect to inhibit the growth of Candida sp.  Purpose: To evaluate the antifungal susceptibility of TTO 5% compared with nystatin against Candida sp. taken from HIV/AIDS patients. Methods: The study assessed the antifungal effect of TTO 5% and nystatin against 33 isolates of Candida sp. that was isolated from the oral cavity of 30 HIV/AIDS patients in Dr. Soetomo General Academic Teaching Hospital Surabaya, Indonesia. Antifungal susceptibility was evaluated by the disc diffusion method. The diameter of the inhibition zone was determined as the result of this study. Result: The most strains found in this study was Candida albicans (84.8%). The mean inhibition zone of nystatin against all strains was 23.24 mm whether it was 17.55 mm for TTO 5%. The inhibition zone of both TTO 5% and nystatin in Candida non-albicans were tended to be higher than in Candida albicans. It revealed that the inhibition zone of TTO 5% significantly lower than nystatin (P = 0.00; P < 0.05).Conclusion: Therefore, the antifungal susceptibility of nystatin was significantly higher than TTO 5% against Candida albicans and non-albicans.

Most read articles by the same author(s)

1 2 3 4 5 6 7 8 9 10 > >>