Antifungal Activity of Rosmarinus Officinalis Essential Oil and Nystatin on Store Isolate of Candida species from HIV/AIDS Patients with Oral Candidiasis

Bernadya Yogatri Anjuwita, Iskandar Zulkarnain, Muhammad Yulianto Listiawan, Evy Ervianti, Rahmadewi Rahmadewi, Pepy Dwi Endraswari, Damayanti Damayanti, Dwi Murtiastutik

= http://dx.doi.org/10.20473/bikk.V32.3.2020.167-173
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Abstract


Background: Oral candidiasis is caused by the mycotic activity of Candida albicans present in the oral cavity, and it is one of the most common opportunistic infections found in patients with Human Immunodeficiency Virus (HIV)/acquired immune deficiency syndrome (AIDS). The growing resistance and side effects to common antifungal drugs have promoted herbal essential oils as antifungal agents in recent years. In this study, essential oils (EO) of Rosmarinus officinalis (Lamiaceae) were examined for in vitro antifungal activ­ity against Candida species. Purpose: To evaluate the antifungal activity of essential oils of Rosmarinus officinalis (Lamiaceae) and nystatin using the microdilution technique by determining the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of Candida spesies. Methods: This was an experimental laboratory study with a post-test-only design conducted in Dr. Soetomo General Academic Teaching Hospital, Surabaya. Forty isolates consisted of twenty isolates of Candida albicans and twenty isolates of Candida non-albicans were collected. The isolates were tested for antifungal activity using the microdilution on 96-well plates. Result: There was a significant difference from the results of the MIC concentration of rosemary essential 100% to 6.25% microdilution method between nystatin and rosemary essential oil (p < 0.05). Conclusion: The antifungal activity of rosemary essential oil was better than nystatin that the lowest MIC value, which was 6.25%, has been obtained the microdilution method. The minimum fungicidal concentration of rosemary essential oil was 25%, while the minimum fungicidal concentration nystatin was higher than 100%.


Keywords


Antifungal activity; Candida albicans; Candida non-albicans; essential oil; nystatin; Rosmarinus officinalis;

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References


Abdulaziz SM, Abdulrahman I, Aso S, Muhammad A. In vitro antifungal activity of essential oils from local plants against fluconazole-resistant oral Candida albicans isolates. Zanco J. Med. Sci 2015; 19(2):965-71.

Bandeira H, Santos DP, Cavalcanti YW, Francisco C, Nonaka W, Castro R. Antifungal Activity of Eugenol and its Association with Nystatin on Candida albicans Antifungal Activity of Eugenol and its Association with Nystatin on Candida albicans. Pesq Bras Odontoped Clin Integr 2017; 17(1):1-8.

Sakkas H, Gousia P, Economou V, Petsios S, Sakkas H. Antifungal activity of four essential oils against Candida clinical isolates Antifungal activity of four essential oils against Candida clinical isolates . 2016; Asian J of Ethnopharm and Med Foods; 2(1):22-5

Jahani S, Bazi S, Shahi Z, Asadi MS, Mosavi F, Baigi GS. Antifungal Effect of the Extract of the Plants Against Candida albicans. Int J Infect 2017; 4(2): 1–4.

Calvacanti WY, Padilha W wilney. Anti-adherent activity of Rosmarinus officinalis essential oil on Candida albicans : an SEM analysis Atividade antiaderente do óleo essencial de Rosmarinus officinalis. Rev Odonto Cienc 2011; 26(2): 139–44.

Carvalhinho S, Margarida A. Susceptibilities of Candida albicans Mouth Isolates to Antifungal Agents , Essentials Oils and Mouth Rinses Susceptibilities of Candida albicans Mouth Isolates to Antifungal Agents , Essentials Oils and Mouth Rinses. Mycopatol 2012; 174(1): 69-76.

Patturaja K. Evaluation of Antimycotic Activity of Three Essential Oils on Candida Albicans -An Invitro Study. J Pharm Sci  Res 2017; 9(4): 480–2.

Nejati H, Farahpour MR. disseminated Candida albicans infection in rat model Topical Rosemary officinalis essential oil improves wound healing against disseminated Candida albicans infection in rat model. Comp Clin Pathol 2015; 1(1):1-8

Bouzid S. Chemical Composition of the Essential Oil of Rosemary ( Rosmarinus officinalis L .) of Tunisian Origin. Asian J Chemist 2013; 25(5): 2601–3.

Nenoff P, Krüger C, Neumeister C, Schwantes U, Koch D. In vitro susceptibility testing of yeasts to nystatin – low minimum inhibitory concentrations suggest no indication of in vitro resistance of Candida albicans , Candida species or non-Candida yeast species to nystatin. Clin Med Invest 2016; 1(3): 71–6.

Oliveira JR De, Esteves S, Camargo A. Rosmarinus officinalis L . ( rosemary ) as therapeutic and prophylactic agent. J of Biomed Science 2019; 8(1): 1–22.

Aminasih N, Sriviona R. Isolasi Senyawa Antijamur Dari Rimpang Lengkuas Putih ( Alpinia galanga ( L .) Willd ) Dan Penentuan Konsentrasi Hambat Minimum Terhadap Candida albicans. Semirata FMIPA Univ Lamp 2013; 1(1):301–8.

Massa N, Cantamessa S, Novello G, Ranzato E. Antifungal activity of essential oils against azole-resistant and azole- susceptible Candida glabrata strains vaginal isolates Sensitivity of Candida albicans to essential oils : are they an alternative to antifungal agents?. Can J Microbiol 2018; 64(10):647-63.

Matsuzaki Y, Tsujisawa T, Nishihara T, Nakamura M, Kakinoki Y. Antifungal activity of chemotype essential oils from rosemary against Candida albicans. Open J of Somatolog 2013;3(1): 176–82.

Khorram Z, Hakimaneh S, Naeini A, Rafieinezhad R, Salari AM, Shayegh SS. The Antifungal Effects of Two Herbal Essences in Comparison with Nystatin on the Candida Strains Isolated from the Edentulous Patients. J Contemp Dent Pract 2019;20(6):4–7.


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