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Submitted
11 October 2018
Accepted
11 October 2018
Published
12 October 2018
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Abstract

This study was designed to examine motor and congnitive changes, infarct lesion and neurohistological changes, involving histologic staining and immunohistochemical expression of caspase-3 after induction by right unilateral common carotid artery occlusion (RUCCAO) for 90 minutes. The animals were divided into two groups: sham group and stroke model group. Cognitive impairment was evaluated by Y maze. Motor function was measured on days 0, 1, 3 and 7 using FUAT paradigm. Infarct area, histological and caspase-3 expressions were evaluated on day 14 after RUCCAO. The results showed that RUCCAO induced cognitive and motor impairment on day 3 and 7. Furthermore, stroke model group induced infarct lesion. Hispatology examination showed body damage of neuron cell in the ipsilateral hemisphere. Moreover, expression of caspase-3 on RUCCAO group was significantly higher than that in sham group. In conclusion, RUCCAO method caused significant cognitive and motor function impairment. Furthermore, RUCCAO also induced infarct lesions and cell death in the thalamus brain area. Thus, RUCCAO can be employed as a method for ischemic stroke model, especially in focal ischemia.

Keywords

Ischemic stroke right unilateral common carotid artery occlusion Caspase 3 rats

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  • Folia Medica Indonesiana is a scientific peer-reviewed article which freely available to be accessed, downloaded, and used for research purposes. Folia Medica Indonesiana (p-ISSN: 2541-1012; e-ISSN: 2528-2018) is licensed under a Creative Commons Attribution 4.0 International License. Manuscripts submitted to Folia Medica Indonesiana are published under the terms of the Creative Commons License. The terms of the license are:

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How to Cite
Mentari, I. A., Naufalina, R., Rahmadi, M., & Khotib, J. (2018). Development Ischemic Stroke Model by Right Unilateral Common Carotid Artery Occlusion (RUCCAO) Method. Folia Medica Indonesiana, 54(3), 200–206. https://doi.org/10.20473/fmi.v54i3.10015
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References

  1. Casals JB, Pieri NCG, Feitosa MLT, Ercolin ACM, Roballo KCS, Barreto RSN, Bressan FF, Martins DS, Miglino MA, Ambrósio CE (2011). The use of animal models for stroke research: A review. Comparative Medicine 61, 305-313
  2. Chiang T, Messing RO, Chou WH (2011). Mouse model of middle cerebral artery occlusion. Journal of Visualized Experiments, 2761
  3. Devinsky O, D'Esposito M (2004). Neurology cognitive and behavioral disorder. emotion and the system limbic. US New York, Oxford University Press, p 330-351
  4. Fluri F, Schuhmann MK, Kleinschnitz C (2015). Ani-mal models of ischemic stroke and their application in clinical research. Drug Design, Development and Therapy, 3445-3454
  5. Guo H, Itoh Y, Toriumi H, Yamada S, Tomita Y, Hoshino H, Suzuki N (2011). Capillary remodeling and collateral growth without angiogenesis after unilateral common carotid artery occlusion in mice. Microcirculation 18, 221-227
  6. Hua Y, Schallert T, Keep RF, Wu J, Hoff JT, Xi G (2002). Behavioral tests after intracerebral hemor-rhage in the rat. Stroke 33, 2478-84
  7. Kaushal V, Herzog C, Haun RS, Kaushal GP (2014). Caspase Protocols in Mice. Methods Mol Biol 1133, 141-154
  8. Kumar A, Aakriti, Gupta V (2016). A review on animal models of stroke: An update. Brain Research Bulletin S0361-9230, 30029-6
  9. Leach KR (2012). The use of forelimb asymmetry functional tests to determine motor recovery with vari-ous drug treatments following endothelin-induced stroke. Browse all Theses and Dissertations. Paper 1092
  10. Liu S, Levine SR, Winn HR (2010). Targeting ischemic penumbra: Part I - From pathophysiology to thera-peutic strategy. Journal of Experimental Stroke and Translational Medicine 31, 47-55
  11. Mehta SL, Vemuganti R (2014). Mechanisms of stroke induced neuronal death: Multiple Therapeutic Oppor-tunities. Advances in Animal and Veterinary Sciences, 438
  12. Nguyen AQ, Cherry BH, Scott GF, Ryou MG, Mallet RT (2014). Erythropoietin: Powerful protection of ischemic and post-ischemic brain. Experimental bio-logy and medicine (Maywood, N.J.), 1461-1475.
  13. Onaolapo OJ, Onaolapo AY, Mosaku TJ, Akanji OO, Abiodun OR (2012). Elevated plus maze and Y-maze behavioral effects of subchronic, oral low dose mono-sodium glutamate in Swiss Albino mice. (IOSR-JPBS) ISSN 2278-3008 3, 21-27
  14. Rupadevi M, Parasuraman S, Raveendran R (2011). Protocol For middle cerebral artery occlusion by an intraluminal suture method. Journal of Pharmacology & Pharmacotherapeutics 2, 36-39
  15. Tsai TH, Lu CH, Wallace CG, Chang WN, Chen SF, Huang CR, Tsai NW, Lan MY, Sung PH, Liu CF, Yip HK (2015). Erythropoietin improves long-term neuro-logical outcome in acute ischemic stroke patients: A randomized, prospective, placebo-controlled clinical trial. BioMed Central 19, 49
  16. Velier JJ, Ellison JA, Kikly KK, Spera PA, Barone FC, Feurstein GZ (1999). Caspase-8 and caspase-3 are expressed by different populations of cortical neurons undergoing delayed cell death after focal stroke in the rat. The Journal of Neuroscience 19, 5932-5941
  17. Wahl F, Allix M, Plotkine M, Boulu RG (1992). Neuro-logical and behavioral outcomes of focal cerebral ischemia in rats. Stroke 23
  18. Waxman SG (2007). The limbic system. In: Clinical neuroanatomy. The limbic system. NewYork, The MacGraw-Hill Companies, p 229-239
  19. Woodruff TM, Thundyil J, Tang SC, Sobey CG, Taylor SM, Arumugam TV (2011). Pathophysiology, trea-tment, and animal and cellular models of human ischemic stroke. Molecular Neurodegeneration, 1-3
  20. Yoshizaki K, Adachi K, Kataoka S, Watanabe A, Tabira T, Takahashi K, Wakita H (2008) Chronic cerebral hypoperfusion induced by right unilateral common carotid artery occlusion causes delayed white matter lesions and cognitive impairment in adult mice . Experimental Neurology, 585-591

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