THE POTENCY OF ALPHA LIPOIC ACID AS ANTI INFLAMMATORY ON THE COMPLETE FREUND'S ADJUVANT-INDUCED RHEUMATOID ARTHRITIS IN RAT MODEL

Selvi Megawati, Mahardian Rahmadi, Imam Susilo, Junaidi Khotib

= http://dx.doi.org/10.20473/fmi.v52i2.5219
Abstract views = 827 times | downloads = 337 times

Abstract


Rheumatoid arthritis (RA) is an autoimmune diseases which is characterized by chronic inflammation of the synovial tissue in joints. This research was designed to investigate the effect of alpha lipoic acid as antioxidant on rats with complete freund’s adjuvant (CFA)-induced RA by intra articular injection of complete freund’s adjuvant (CFA). ALA was administered orally once a day for 7 days at 30, 60 and 120 mg doses a week after CFA injection. The severity of arthritis was evaluated by joint diameter and latency time on thermal stimulation. Joint diameter and latency time on thermal stimulation will measured on day 0, 3, 5, 7, 10, 12 and 14. Measurement of malondialdehyde (MDA) level in plasma was performed using thiobarbituric acid (TBA) method to assess lipid peroxidation. Histology of joint was examined by microscope following hematoxylin-eosin staining. The result showed that treatment with ALA at 30 mg and 60 mg significantly decreased the joint diameter compared to CFA group (p=0.003; p=0.001 respectively) and rat’s latency time on thermal stimulation was also significantly increased compared to CFA group (p=0.015; p=0.026 respectively). Measurement of MDA in CFA group and ALA group had no significant difference. Histological staining indicated that the recovery of the synovial membranes of joint in ALA group had no effect. Results indicated that ALA has the effect to suppress the development of inflammation in RA but not through oxidative stress pathway.

Keywords


Rheumatoid Arthritis, complete freund’s adjuvant, alpha lipoic acid, antioxidant

Full Text:

PDF

References


Afonso V, Champy R, Mitrovic D, Collin P, Lomri A (2007). Reactive oxygen species and superoxide dismutases: role in joint diseases. Joint Bone Spine 74, 324-329

Bhowmick K, Chakraborti G, Gudi NS, Moideen AVK, Sheety HV (2008). Free radical and antioxidant status in rheumatoid arthritis. Indian Journal of Rheumatology 3, 8-12

Capitanescu B, Simionescu C, Margaritesu C, Stepan A, Ciurea R (2011). Clinical and morphological aspects of sinovitis early rheumatoid arthritis. Current Health Sciences Journal 37, 17-20

Chen L, Bao B, Wang N, Xie J, Wu W (2012). oral administration of shark type ii collagen suppresss complete freund’s adjuvant-induced rheumatoid arthritis in rats. Pharmaceuticals 5, 339-352

Dubey S and Adebajo AO (2008). Historical and current perspectives on management of osteorthritis and rheumatoid arthritis. In: Reid DM and Miller CG (eds). Clinical Trials in Rheumatoid Arthritis and Osteoarthritis, London, Springe, p 12-13

Emery P (2011). Pocket Reference to Early Rheumatoid Arthritis, London, Springer Healthcare, p 1-4

Golbidi S, Badran M, Laher I (2011). Diabetes and alpha lipoic acid. Frontiers in Pharmacology 2, 1-15

Haywood L and Walsh D (2001). Vasculature of the normal and arthritis synovial joint. Histology and Histopathology Cellular and Molecular Biology 16, 277-284

Henrotin YE, Bruckner P, Pujol JPL (2003). The role of reactive oxygen species in homeostasis and degradation of cartilage. Osteoarthritis and Cartilage 11, 747-755

Ishibashi T (2013). Molecular hydrogen: new antioxidant and anti-inflammatory therapy for rheumatoid arthritis and related diseases. Current Pharmaceutical Design 19, 6375-6381

Jaswal S, Mehta HC, Sood AK, Kaur J (2003). Antioxidant status in rheumatoid arthritis and role of antioxidant therapy. Clinica Chimica Acta 338, 123-129

Malemud CJ (2009). Intracellular signaling pathways in rheumatoid arthritis. Journal of Clinical and Cellular Immunology 4, 1-15

Miossec P and Tebib J (2006). Diagnosis of rheumatoid arthritis at an early stage. In: Bouysset M, Tourne Y, Tillmann K (eds). Foot and Ankle in Rheumatoid Arthritis, Berlin, Springer-Verlag, p 1-3

Mirshafiey A and Mohsenzadegan M (2008). The role of reactive oxygen species in immunopathogenesis of rheumatoid arthritis. Iranian Journal of Allergy, Asthma and Immunology 4, 195-202

Mirtaheri E, Gargari BP, Kolahi S, Dehghan P, Asghari-Jafarabadi M, Hajalilou M, Novin ZS, Abbasi MM (2015). Effects of alpha lipoic acid supplemetation on inflammatory biomarkers and matrix metalloproteinase-3 in rheumatoid arthritis patiens. Journal of the American College of Nutrition 34, 310-317

Mor A, Abramson SB, Pillinger MH (2005). The fibroblast-like synovial cell in rheumatoid arthritis: a key player in inflammation and joint destruction. Clinical Immunology 115, 118-128

Orhan CE, Onal A, Uyanikgil Y, Ulker S (2013). Antihyperalgesic and antiallodynic effect of sirolimus in rat model of adjuvant arthritis. European Journal of Pharmacology 705, 35-41

Saxena R (2014). Arthritis as a disease of aging and changes in antioxidant status. In: Preedy VR (ed). Aging: Oxidative Stress and Dietary Antioxidants, London, Academic Press, p 52

Shay KP, Moreau RF, Smith EJ, Smith AR, Hagen TM (2009). Alpha-lipoic acid as a dietary supplement: molecular mechanisms and therapeutic potential. Biochimica et Biophysica Acta (BBA)-General Subjects 1790, 1149-1160

Snekhalatha U, Anburajan M, Venkatraman B, Menaka M (2012). Evaluation of complete freund’s adjuvant-induced arthritis in a wistar rat model (comparison of thermography and histopathology). Zeitschrift für Rheumatologie 4, 375-382

Stadtman ER and Berlett BS (2002). Reactive oxygen-mediated protein oxidation in aging and disease. In: Gilbert DL and Colton CA (eds). Reactive Oxygen Species in Biology Systems: An Interdisciplinary Approach, New York, Kluwer Academic Publishers, p 660

Wahl K and Schuna AA (2014). Rheumatoid arthritis. In: Dipiro JT, Talbert RL, Yee GC, Matzke GR, Wells BG, Posey LM (eds). Pharmacotherapy: A Pathophysiologic Approach, New York, Mc Graw Hill, p 3201-3203

Waldburger J and Firestein GS (2009). Signaling pathways in rheumatoid arthritis. In: Tak P (ed). New Therapeutic Targets in Rheumatoid Arthritis, Switzerland, Birkhauser Verlag, p 167-168

Zhang R and Ren K (2011). Animal models of inflammatory pain. In: Ma C and Zhang J (eds). Animal Models of Pain, London, Humana Press, p 23-30


Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Folia Medica Indonesiana



                   

  

 

View My Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License