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Submitted
27 January 2019
Accepted
2 January 2023
Published
5 March 2023
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Abstract

Highlights:



  1. Rectal and vaginal swab specimens were collected from pregnant women, and there was no significant difference in the proportions of group B Streptococcus isolation.

  2. Combined vaginal and rectal swab cultures provide a higher isolation of group B Streptococcus.


Abstract :


Group B Streptococcus is a Gram-positive bacterium found in women. It causes high-risk mortality in pregnant women, newborns, and the elderly. This study aimed to compare group B Streptococcus (GBS/Streptococcus agalactiae) proportions from different collection sites (vaginal and rectal swabs). This was an analytic observational study with a hospital-based cross-sectional design. A total of 74 swabs were taken from 37 pregnant women at 35–37 weeks of gestation. Each participant provided a vaginal swab and a rectal swab, which were cultured in Todd Hewitt broth, blood agar, and CHROMagar. The specimens were subsequently identified using the VITEK 2 system. The GBS isolation percentages from the vaginal and rectal swab specimens were determined to be 13.5% and 8.1%, respectively. The McNemar test had a result of 0.697, and the Cohen's kappa test had a result of 0.165. To conclude, there was no significant difference in GBS isolation proportions between the vaginal and rectal swab cultures. Combined vaginal and rectal swab cultures were required to increase GBS isolation from pregnant women.


 

Keywords

Proportion of Streptococcus agalactiae vaginal swab rectal swab maternal health

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How to Cite
Ivanna, Wasito, E. B., & Debora, K. (2023). Proportions of Group B Streptococcus Isolation from Pregnant Women’s Vaginal and Rectal Swab Specimens at a Tertiary Hospital in Surabaya, Indonesia. Folia Medica Indonesiana, 59(1), 46–50. https://doi.org/10.20473/fmi.v59i1.11708
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References

  1. Africa CWJ, Kaambo E (2018). Group B Streptococcus serotypes in pregnant women from the Western Cape Region of South Africa. Frontiers in Public Health 6. doi: 10.3389/fpubh.2018.00356.
  2. Bidgani S, Navidivar T, Najafian M, et al (2016). Comparison of group B Streptococci colonization in vaginal and rectal specimens by culture method and polymerase chain reaction technique. Journal of the Chinese Medical Association 79, 141–145. doi: 10.1016/j.jcma.2015.06.021.
  3. Edwards JM, Watson N, Focht C, et al (2019). Group B Streptococcus (GBS) colonization and disease among pregnant women: A historical cohort study. Infectious Diseases in Obstetrics and Gynecology, 1–6. doi: 10.1155/2019/5430493.
  4. Gopal Rao G, Nartey G, McAree T, et al (2017) Outcome of a screening programme for the prevention of neonatal invasive early-onset group B Streptococcus infection in a UK maternity unit: An observational study. BMJ Open 7, e014634. doi: 10.1136/bmjopen-2016-014634.
  5. Hanifah NR, Andrianto A, Trijanto B (2018). Screening, counseling and referral pattern of structural heart disease in pregnancy cases at public health center in Surabaya. Majalah Obstetri & Ginekologi 25, 103. doi: 10.20473/mog.V25I32017.103-112.
  6. Hanson L, VandeVusse L, Malloy E, et al (2022) Probiotic interventions to reduce antepartum Group B Streptococcus colonization: A systematic review and meta-analysis. Midwifery, 103208. doi: 10.1016/j.midw.2021.103208.
  7. Khalil MR, Uldbjerg N, Thorsen PB, et al (2017). Intrapartum PCR assay versus antepartum culture for assessment of vaginal carriage of group B Streptococci in a Danish cohort at birth. PLOS ONE. Edited by J. Melo-Cristino 12, p. e0180262. doi: 10.1371/journal.pone.0180262.
  8. Kurian NK, Modi D (2022). Mechanisms of group B Streptococcus-mediated preterm birth: Lessons learnt from animal models. Reproduction and Fertility 3, R109–R120. doi: 10.1530/raf-21-0105.
  9. Kurniawati EM, Toma F, Parathon H, et al (2019). Incidence of bacterial vagniosis and aerobic vaginitis before and after pessary insertion in Indonesia. Indian Journal of Public Health Research & Development 10, 1680. doi: 10.5958/0976-5506.2019.03084.5.
  10. Kwatra G, Madhi SA, Cutland CL, et al (2013). Evaluation of trans-vag broth, colistin-nalidixic agar, and CHROMagar strepB for detection of group B Streptococcus in vaginal and rectal swabs from pregnant women in South Africa. Journal of Clinical Microbiology 51, 2515–2519. doi: 10.1128/jcm.00251-13.
  11. Linggarjati S, Parti DD, Sakinah EN (2021). Antibiotic sensitivity on pathogenic bacteria causing bacterial vaginosis. Majalah Obstetri & Ginekologi 29, 18. doi: 10.20473/mog.V29I12021.18-22.
  12. Nadeau HCG, Bisson C, Chen X, et al (2022). Vaginal-perianal or vaginal-perineal compared with vaginal-rectal culture-based screening for Group B Streptococci (GBS) colonization during the third trimester of pregnancy: A systematic review and meta-analysis. BMC Pregnancy and Childbirth 22, 204. doi: 10.1186/s12884-022-04546-w.
  13. Sulikah SRO, Hasanah M, Setyarini W, et al (2022). Occurrence of carriage of multidrug resistant enterobacteriaceae among pregnant women in the primary health center and hospital setting in Surabaya, Indonesia. Microbial Drug Resistance 28, 48–55. doi: 10.1089/mdr.2020.0506.
  14. Raabe VN, Shane AL (2019). Group B Streptococcus (Streptococcus agalactiae). Microbiology Spectrum. Edited by Fischetti VA, et al, 7. doi: 10.1128/microbiolspec.GPP3-0007-2018.
  15. Rosa-Fraile M, Spellerberg B (2017). Reliable detection of group B Streptococcus in the clinical laboratory. Journal of Clinical Microbiology. Edited by C. S. Kraft 55, 2590–2598. doi: 10.1128/jcm.00582-17.
  16. Russell NJ, Seale AC, O'Driscoll M, et al (2017) Maternal colonization with group B Streptococcus and serotype distribution worldwide: Systematic review and meta-analyses. Clinical Infectious Diseases 65, S100–S111. doi: 10.1093/cid/cix658.
  17. Safari D, Gultom SM, Tafroji W, et al (2021). Prevalence, serotype and antibiotic susceptibility of Group B Streptococcus isolated from pregnant women in Jakarta, Indonesia. PLOS ONE. Edited by Karunasagar I 16, e0252328. doi: 10.1371/journal.pone.0252328.
  18. Simanungkalit N, Handayani S, Akbar MIA (2021). Poedji Rochjati Score Card as media for preconception counseling. Folia Medica Indonesiana 57, 310. doi: 10.20473/fmi.v57i4.26818.
  19. Sri-Budayanti N, Hariyasa-Sanjaya N (2013). Group-B Streptococcus in pregnant women: Prevalence of colonization and sensitivity pattern in Denpasar during June 2007-May 2008. Bali Medical Journal 2, 17–20. doi: https://doi.org/10.15562/bmj.v2i1.33.
  20. Suwardewa TGA, Sanjaya INH, Anantasika AAN, et al (2022). Correlation between group B Streptococcus infection in the vagina with maternal serum C-reactive protein levels in preterm labor. European Journal of Medical and Health Sciences 4, 18–21. doi: 10.24018/ejmed.2022.4.6.1452.
  21. Szymusik I, Kosińska KK, Pietrzak B, et al (2014). Do we need a different approach to GBS screening? Polish Gynaecology 85. doi: 10.17772/gp/1753.
  22. Tille P (2014). Streptococcus, Enterococcus, and similar organisms in: Bailey and Scott's Diagnostic Microbiology 13th ed.
  23. Turner G, O'Grady M, Hudson D, et al (2022). Rectal swabs are a reliable method of assessing the colonic microbiome. International Journal of Medical Microbiology 312, 151549. doi: 10.1016/j.ijmm.2022.151549.
  24. Wang CH, Kung WJ, Lee CH, et al (2022). High rates of colonization and antimicrobial resistance of group B Streptococcus highlight the need for vaccination even after implementation of guidelines for intrapartum antibiotic prophylaxis. Vaccine 40, 282–287. doi: 10.1016/j.vaccine.2021.11.069.

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