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The use of a ketogenic diet with an increased proportion of fat is rampant lately, both as a therapy or even lifestyle. The use of a ketogenic diet is feared to cause dyslipidemia. It will also lead to atherosclerosis and thrombosis. This study aimed to determine the effect of a high-fat diet on HDL, LDL, and HDL/LDL ratio in mice. Thirty male Mus Musculus, 2-3 mo, 15-25 g divided into five groups diet. K1 (12% fat, 20% protein, 62% carb), K2 (30% fat, 60%protein), K3 (45% fat, 45% protein), K4 (60% fat, 30% protein) and K5 (75% fat, 15% protein) for 4 weeks. Feed and water by adlibitum. Blood for serum was taken from the ventricle, while in the measurement of HDL serum level, the LDL used ELISA. Normality of data analyzed by Shapiro Wilk, and homogeneity by Levene test. HDL serum was analyzed using equality test followed by Games Howell, LDL serum and ratio of HDL/LDL was tested by ANOVA followed by LSD with significance 0,05. HDL serum in K1 (62,50±9.94) mg/dL, K2 (78,40±18,76) mg/dL, K3 (79,00±3,81) mg/dL, K4 (80,00±2,16) mg/dL, and K5 (83,50±5,62) mg/dL with p<0,05 in K1, K2 to K3, K4 and K5. LDL serum in K1 (21,67± 4,80) mg/dL, K2 (23,00±12,70) mg/dL, K3 (18,40±4,34) mg/dL, K4 (24,00 ±1,83) mg/dL and K5 (22,00 ± 4,08) mg/dL with p>0,05. Ratio HDL/LDL K1 (3,01±0,91), K2 (4,10±1,86), K3 (4,53±1,5), K4 (3,35±0,34), and K5 (3,96 ± 1,25) with p>0,05.


(high fat diet mice HDL LDL HDL/LDL ratio)

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Laksana, I., Rejeki, P. S., Herawati, L., Al Arif, M. A., & Wardhani, I. L. (2021). High-Fat Diet Increases Serum HDL, but Not for LDL and HDL/LDL Ratio in MICE. Folia Medica Indonesiana, 57(2), 117–120.


  1. Burén J, Ericsson M, Damasceno NRT, et al (2021). A ketogenic low-carbohydrate high-fat diet increases LDL cholesterol in healthy, young, normal-weight women: A randomized controlled feeding trial. Nutrients 13, 1-12.
  2. Chiu S, Williams PT, Krauss RM (2017). Effects of a very high saturated fat diet on LDL particles in adults with atherogenic dyslipidemia: A randomized controlled trial. PLOS ONE 12, 1-14.
  3. Dashti HM, Mathew TC, Hussein T, et al (2004). Long-term effects of a ketogenic diet in obese patients. Experimental and Clinical Cardiology 9, 200-5.
  4. Hu T, Yao L, Reynolds K, et al (2015). The effects of a low-carbohydrate diet vs. a low-fat diet on novel cardiovascular risk factors: A randomized controlled trial. Nutrients 7, 7978-94.
  5. Jensen NJ, Wodschow HZ, Nilsson M, et al (2020). Effects of ketone bodies on brain metabolism and function in neurodegenerative diseases. International Journal of Molecular Sciences 21, 1-17.
  6. Kosinski C, Jornayvaz FR (2017). Effects of ketogenic diets on cardiovascular risk factors: Evidence from animal and human studies. Nutrients 9, 1-16.
  7. Lima PA, de Brito SLP, Damasceno NRT (2015). Ketogenic diet in epileptic children: Impact on lipoproteins and oxidative stress. Nutr. Neurosci 18, 337-344.
  8. Masood W, Annamaraju P, Uppaluri KR (2020). Ketegonic diet. Treasure Islands (FL), StatPearls Publishing, p 1.
  9. Ministry of Health (2013). Basic health research, Jakarta, Balitbang Kemenkes RI.
  10. Noain JS, Minupuri A, Kulkarni K, et al (2020). Significant impact of the ketogenic diet on low-density lipoprotein cholesterol levels. Cureus 12, 1-4.
  11. Oyekale AS (2019). Effect of obesity and other risk factors onhypertension among women of reproductive age inghana: an instrumental variable probit model. International Journal of Environmental Research and Public Health, 16, 1-17.
  12. Paoli A, Rubini A, Volek JS, et al (2013). Beyond weight loss: A review of the therapeutic uses of very-low-carbohydrate (ketogenic) diets. European Journal of Clinical Nutrition 67, 789-96.
  13. Salvador AC, Arends D, Barrington WT, et al (2021). Sex-specific genetic architecture in response to American and ketogenic diets. International Journal of Obesity 45, 1-14.
  14. Volek JS, Sharman MJ, Forsythe CE (2005). Modification of lipoproteins by very low-carbohydrate diets. J Nutr 2005, 6, 1339-1342.
  15. Westman EC, Yancy WS, Mavropoulos JC, et al (2008). The effect of a low-carbohydrate, ketogenic diet versus a low-glycemic index diet on glycemic control in type 2 diabetes mellitus. Nutrition & Metabolism 5, 1-9.

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