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Submitted
4 February 2020
Published
1 June 2021
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Abstract

Pain affects millions of people and is one of the most frequent complaints in the medical office. Pain involves various behavioral and emotional aspects. When it is persistent, it can become debilitating, impairing occupational performance, and producing negative impacts for the economy and public health costs. In general, commercial drugs can improve chronic pain patients' quality of life which can result in adverse reactions. Therefore, the search for new capsules as an opportunity remedy for ache is a challenge. Pain occurs due to stimuli from pain receptors called nociceptors. One pain known in the medical world is orofacial, defined as pain that occurs in soft and hard tissue in the head, face, and neck area. This study determined the antinociceptive effect of flavonoids isolated from the Leea Indica plant, on orofacial pain in zebrafish (Danio rerio) as a test model for behavior or locomotor activity. To study the orofacial pain of zebrafish induced with glutamate, this study was a true experiment. The parameter used was the number of times the fish crossed the line between the caudran from the glass petri dish during 0-5 minutes and 15-30 minutes. The Anova one-way test showed that there were differences in locomotors activity measured from 0-5 minutes and 15-30 minutes by giving flavonoids that could affect zebrafish locomotors activity or an increase in zebrafish locomotors activity. The antinociceptive effect of flavonoids was similar to tramadol. The flavonoids from Leea Indica had an antinociceptive effect on orofacial pain in adult zebrafish. The flavonoid dose of 2.5 mg/ml was a dose that had a significant difference in all treatment groups.

Keywords

Leea indica flavonoids zebrafish.

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  • Folia Medica Indonesiana is a scientific peer-reviewed article which freely available to be accessed, downloaded, and used for research purposes. Folia Medica Indonesiana (p-ISSN: 2541-1012; e-ISSN: 2528-2018) is licensed under a Creative Commons Attribution 4.0 International License. Manuscripts submitted to Folia Medica Indonesiana are published under the terms of the Creative Commons License. The terms of the license are:

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How to Cite
Daya, M. P., Saleh, A. Y., & Astari, R. V. (2021). The Effect of Antinociceptive Flavonoid on Leea Indica Leaves for Orofacial Pain of Adult Zebra Fish (Danio Rerio). Folia Medica Indonesiana, 57(2), 135–142. https://doi.org/10.20473/fmi.v57i2.17711
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References

  1. Bachour RL, Golovko O, Kellner M, et al (2020). Behavioral effects of citalopram, tramadol, and binary mixture in zebra fish (Danio Rerio) larvae. Chemosphere 238, 0-7.
  2. Correia AD, Cunha SR, Scholze M, et al (2011). A novel behavioral fish model of nociception for testing analgesics. Pharmaceuticals (Basel) 4, 665-680.
  3. Curtright A, Rosser M, Goh S, et al (2015). Modeling nociception in zebra fish: A way forward for unbiased analgesic discovery. PLoS ONE 10, 1-18.
  4. do Nascimento JET, de Morais SM, de Lisboa DS, et al (2018). The orofacial antinociceptive effect of Kaempferol-3-O-rutinoside, isolated from the plant Ouratea fieldingiana, on adult zebra fish (Danio Rerio). Biomedicine and Pharmacotherapy 107, 1030-1036.
  5. Ghagane SC, Puranik SI, Kumbar VM, et al (2017). In vitro antioxidant and anticancer activity of Leea Indica leaf extracts on human prostate cancer cell lines. Integrative Medicine Research 6, 79-87.
  6. Gut P, Reischauer S, Stainier DYR, et al (2017) Little fish, big data: Zebra fish as a model for cardiovascular and metabolic disease. Physiological Reviews 97, 889-938.
  7. Klasser GD, Goulet JP, Laat AD, Manfredini D (2017). Classification of orofacial pain. In: Farah CS, Balasubramaniam R, McCullough MJ (eds). (2019). Contemporary oral medicine, Switzerland, Springer Nature.
  8. Magalhí£es FEA, de Sousa CAPB, Santos SAAR, et al (2017) Adult Zebra fish (Danio Rerio): An Alternative Behavioral Model of Formalin-Induced Nociception. Zebrafish 14, 422-429.
  9. Masturoh I, Nauri TA (2018). Metodologi Penelitian Kesehatan, Ministry of Health of the Republic of Indonesia. Available from http://bppsdmk.kemkes.go.id/. Accessed July 19, 2019.
  10. Maulina T, Rachmy CN, Akhter R, et al (2014). The association between self-report of orofacial pain symptoms with age, gender, interference in activities, and socioeconomic factors surveyed in Indonesian community health centers. Asian Pac J Dent 14, 23-34.
  11. Onoja SO, Ezeja MI, Omeh YN, et al (2017). Antioxidant, anti-inflammatory and antinociceptive activities of methanolic extract of Justicia secunda Vahl leaf. Alexandria Journal of Medicine 53, 207-213.
  12. Rahman MA, Imran TB, Islam S (2013). Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea Indica leaf extract. Saudi Journal of Biological Sciences 20, 213-225.
  13. Reddy NS, Navanesan S, Sinniah SK, et al (2012). Phenolic content, antioxidant effect and cytotoxic activity of Leea indica leaves. BMC Complementary and Alternative Medicine 12, 1-7.
  14. Saravanan S, Arunachalam K, Parimelazhagan T (2014). Antioxidant, analgesic, anti-inflammatory and antipyretic effects of polyphenols from Passiflora subpeltata leaves – A promising species of Passiflora Antioxidant, analgesic, anti-inflammatory and antipyretic effects of polyphenols from Passiflora sub. Industrial Crops & Products 54, 272-280.
  15. Saulino M, Kim PS, Shaw E (2014). Practical considerations and patient selection for intrathecal drug delivery in the management of chronic pain. Journal of Pain Research 7, 627-638.
  16. Sharma P, Kumari S, Singh D (2018). Investigating the role of two major dietary flavonoids in zebra fish model of pentylenetetrazol-induced convulsions. Journal of Developing Drugs 7, 026-033.
  17. Shetty A, James L, Nagaraj T, et al (2015). Epidemiology of orofacial pain: A retrospective study. Journal of Advanced Clinical & Research Insights 2, 12-15.
  18. Smiljić S, Savic S, Stevanovic J, et al (2016). Prevalence and characteristics of orofacial pain in university students. Journal of Oral Science 58, 7-13.
  19. Steenbergen PJ, Bardine N (2014). Antinociceptive effects of buprenorphine in zebra fish larvae: An alternative for rodent models to study pain and nociception?. Applied Animal Behaviour Science 152, 92-99.
  20. Wandji BA, Bomba TFD, Awouafack, et al (2016). Antinociceptive effects of the aqueous and methanol extracts of the leaves of Pittosporum mannii Hook. F. (pittosporaceae) in mice. Journal of Ethnopharmacology 187, 224-231.