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27 October 2020
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5 September 2021
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Abstract

This study aimed to explore the phylogenetic pattern of Extended-spectrum β-lactamase-producing Escherichia coli in the gut flora of neonatal into groups A, B1, B2, D and tracing the presence of spreading among the age group of neonatal. Multiplex PCR was conducted to classify the phylogenetic group of ESBL producing E. coli into groups of commensals (A, B1) and pathogen (B2, D). The spread of bacterial agents among the host (neonatal) was conducted by the RAPD-PCR method with two random primers. Among 34 bacterial isolates were identified among early neonatal group A (22,2%), B1 (11,1%), B2 (22,2%) D (33,3%) and postneonatal groups A (8%), B1 (4%), B2 (36%) D (40%). It was not a statistically significant difference (p=0.388). The dominance of pathogenic groups B2 and D as many as 70,6%. From 34 samples was found 34 types of RAPD indicated there were not any bacterial spread among neonatal. The ESBL producing E. coli among early neonatal and postneonatal were dominated by the pathogenic group of B2 and D (total=70,6%), but they were not statistically significant. There was not any spreading of bacterial agents among individual gut flora of neonatal.

Keywords

Escherichia coli ESBL neonate health infection phylogenetic

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  • Folia Medica Indonesiana is a scientific peer-reviewed article which freely available to be accessed, downloaded, and used for research purposes. Folia Medica Indonesiana (p-ISSN: 2541-1012; e-ISSN: 2528-2018) is licensed under a Creative Commons Attribution 4.0 International License. Manuscripts submitted to Folia Medica Indonesiana are published under the terms of the Creative Commons License. The terms of the license are:

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How to Cite
Sulistya, R. B., Kuntaman, K., Koendhori, E. B., Happy, T. A., & Setyarini, W. (2021). Phylogenetic and Transmission Patterns of Extended-Spectrum β- Lactamase –Producing Escherichia Coli among Neonatal’s Gut Flora in Indonesia. Folia Medica Indonesiana, 57(3), 199–204. https://doi.org/10.20473/fmi.v57i3.22828
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References

  1. Barfield WD (2016). Standard terminology for fetal, infant, and perinatal deaths. J Pediatr 137, 1-7.
  2. Bok E, KoË™za´nska A, Mazurek-Popczyk J, et al (2020). Extended phylogeny and extraintestinal virulence potential of commensal escherichia coli from piglets and sows. International Journal of Environmental Research and Public Health, 17,1-17.
  3. Clermont O, Bonacorsi S, Bingen E (2000). Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 66, 4555-4558.
  4. Clermont O, Christenson JK, Denamur E, et al (2013). The clermont escherichia coli phylo-typing method revisited: improvement of specificity and detection of new phylo-groups. Environ Microbiol Rep 5, 58-65.
  5. Darmstadt GL, Zaidi AKM, Stoll BJ (2011). Neonatal infection: A global perspective. Arch Dis Child Fetal Neonatal Ed 90, 221-224.
  6. Das P, Singh AK, Mukherjee S, et al (2013). Composition of escherichia coli population in the neonatal gut: phylogroups and virulence determinants. J Med Microbiol 62, 1680-1687.
  7. Flokas ME, Karanika S, Alevizakos M, et al (2017). Prevalence of ESBL-producing enterobacteriaceae in pediatric bloodstream infections: A systematic review and meta- analysis. PLoS One 12, 1-13.
  8. Happy TA, Setyarini W, Ranuh IGMRG, et al (2020). Prevalence ESBL Producing Escherichia Coli among Children in Indonesia. Indian J of Public Health Research and Development, 11(05), 792-797.
  9. Iranpour D, Hassanpour M, Ansari H, et al (2015). Phylogenetic groups of Escherichia coli strains from patients with urinary tract infection in Iran based on the new clermont phylotyping method. Biomed Res Int, 2015, 1-7.
  10. Kazemnia A, Ahmadi M, Dilmaghani M (2014). Antibiotic resistance pattern of different Escherichia coli phylogenetic groups isolated from human urinary tract infection and avian colibacillosis. Iran Biomed J 18, 219-224.
  11. Luo Q, Wang Y, Xiao Y (2020). Prevalence and transmission of mobilized colistin resistance (mcr) gene in bacteria common to animals and humans. Biosafety and Health 2, 71-78.
  12. Manges AR (2016). Escherichia coli and urinary tract infections: the role of poultry-meat. Clinical Microbiology and Infection 22, 122-129.
  13. Marialouis XA, Santhanam A. (2016). Antibiotic Resistance, RAPD- PCR typing of multiple drug resistant strains of Escherichia coli from urinary tract infection (UTI). J Clin Diagn Res, 10(3), 5–9.
  14. Muinck EJ, De í˜ien T, Storrí¸ O, et al (2011). Diversity, transmission and persistence of Escherichia coli in a cohort of mothers and their infants. Environ Microbiol Rep 3, 352–359.
  15. Neu J, Rushing J (2011). Cesarean versus vaginal delivery: long term infant outcomes and the hygiene hypothesis. Clin Perinatol 38, 321-331.
  16. Oteo J, Cercenado E, Fernández-romero S, et al (2012). Extended-spectrum-B- lactamase-producing Escherichia coli as a cause of pediatric infections: Report of a neonatal intensive care unit outbreak due to a CTX-M-14-producing strain. Antimicrob Agents Chemother 56, 54-8.
  17. Rath S, Dubey D, Suhu MC, et al (2014). Surveillance of ESBL producing multidrug resistant Escherichia coli in a teaching hospital in India. Asian Pac J Trop Dis 4, 140-149.
  18. Storberg V (2014). ESBL-producing Enterobacteriaceae in Africa a non-systematic literature review of research published 2008-2012. Infect Ecol Epidemiol 1, 1-16
  19. Tebruegge M, Pantazidou A, Clifford V (2011). The age-related risk of co-existing meningitis in children with urinary tract infection. PloS ONE 6, 1-6.
  20. Valentine L, Sharp H, Hille K, et al (2014). Subgrouping of ESBL-producing Escherichia coli from animal and human sources: An approach to quantify the distribution of ESBL types between different reservoirs. International Journal of Medical Microbiology 304, 805-816.
  21. Vogel L, Oorschot EV, Maas HM, et al (2000). Epidemiologic typing of Escherichia coli using RAPD analysis, ribotyping and serotyping. Clin Microbiol Infect 6, 82-87.

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