Main Article Content


Infertility is the inability of a partner to get pregnant after regular sexual intercourse without contraception for 12 months. Based on the cause, male infertility is caused by genetic, hormonal, infectious, sexual, and unexplained infertility. However, many infertile couples are classified into unexplained infertility without an antisperm antibody (ASA) immunological examination. This study aimed to prove that couples classified as unexplained infertility can be caused by ASA wives. This was an observational study with a cross sectional study design. The sample consisted of 2 groups, in which the first group consisted of 36 fertile couples and the second group consisted of 35 unexplained infertility couples. All samples were carried out indirect MAR test to obtain the ASA value of the wife. If the wife's ASA value was >40%, the ASA results were positive. Conversely, if the ASA value was <40%, the ASA results were negative. From these 2 groups, there was a statistically significant difference (p<0.0001) that the wife's ASA value in the unexplained infertility group was significantly higher than that in the fertile group. This study concluded that the positive ASA results from the indirect MAR test were significantly found in the unexplained infertility sample.


ASA antisperm antibodies indirect MAR Test

Article Details

How to Cite
Ramadhona, N. H., Hinting, A., & Lunardhi, H. (2020). Differences of Indirect MAR Test in the Unexplained Infertile and Fertile Couples. Folia Medica Indonesiana, 56(1), 1–3.


  1. Bohring C, Krause W (2003). Immune infertility: towards a better understanding of sperm (auto)-immunity. The value of proteomic analysis. Hum. Reprod 18, 915-924
  2. Hinting A, Vermeulen L, Comhair F (1988). The indirect mixed antglobulin reaction tets using a commercially available kit for the detection of antisperm antibodies in serum. Feril Steril 49, 1039-44
  3. Lee HY, Kim JQ, Kim SI (1987). Sperm auto-immunity associated with vasectomy, vasovasostomy and epididymovasostomy in Korean males. Clin.Reprod. Fertil. 5, 347-357
  4. Lombardo F, Gandini L, Dondero F, Lenzi A (2001). Immunology and immunopathology of the male genital tract. Hum. Reprod. Update 7, 450-456
  5. Mahdi BM, Salih WH, Caitano AE, Kadhum BM, Ibrahin DS (2011). Frequency of antisperm in infertile women. J Reprod Infertil 12, 261-265
  6. Mathur S, Goust JM, Williamson HO, Fudenberg HH (1981). Cross-reactivity of sperm and T lymphocyte antigens. Am. J. Reprod. Immunol. 1, 113-118
  7. Nakagawa K, Yamano S, Kamada M, Hinokio K, Maegawa M, Aono T (1999). Quality of embryo does not affect the implantation rate of IVF-ET in infertile women with antisperm antibody. Fertil.Steril 72, 1055-1060
  8. Shi J, Yang Z, Wang M (2007). Screening of an antigen target for immunocontraceptives from cross-reactive antigens between human sperm and Ureaplasmaurealyticum. Infect. Immun. 75, 2004-2011
  9. Silber SJ, Grotjan HE (2004). Microscopic vasectomy reversal 30 years later: A summary of 4010 cases by the same surgeon. J. Androl. 25, 845-859