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Background:Closure of the defect with a random skin flap is constrained by the extent of the defect area. Several studies have shown the benefits of administration of PRP (Platelet Rich Plasma) in random skin flap. The purpose of this study is to compare the effect of PRP injection given before flap elevation, after flap elevation and control on the extended random skin flap procedures in rats.

Methods: This research is experimental with randomized post test only control group design (n = 27, divided into 3 treatment groups) that compare the effectiveness of PRP injection 24 hours prior to the elevation of the flap, after the elevation of the flap, and control in the extended random skin flap in rats. Random skin flap is made in the ratio 1: 5 on the rat skin. Measurement of viable area were observed on days 1, 7 and 14.

Results: There were significantly increased viability of random skin flap on the group with PRP injection 24 hours prior flap elevation compared to other group of treatment. The average of viability on day first 39%±13% ;42%±34%; 62%±14%. On day 7th24%±13%; 36%±26%; 62%±12%.On day 14th16%±15%; 28%±22%; 60%±11%.

Conclusion: Injection of PRP on extended random skin flap on rat 24 hours before flap elevationincrease viability of the flap compared to control group and group which receive PRP injection on extended random flap on rat skin after elevation of the flap. Viability increased due to angiogenesis stimulation after PRP injection.


Platelet Rich Plasma Flap Survival Extended Random Skin Flap

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How to Cite
Sudrajat Wahyu Nugroho, T. E., Rizaliyana, S., & Perdanakusuma, D. S. (2020). Effectiveness of Pre PRP Injection and Post Elevation Flap at Extended Random Flap Rat Skin. Folia Medica Indonesiana, 56(3), 186–191.


  1. Alcántara, D.A., Zaragoza, M.R., Giménez, E.D., Poveda, J., Serrato, B., Gorrea, P. and Juncosa, J. Platelet Rich Plasma: New Insights for Cutaneous Wound Healing Management. J Funct Biomater. 2018 Mar; 9(1): 10.
  2. Carlson N. F. et al. 2002. Platelet Rich Plasma: Clinical Aplication In Dentistry. J Am Dent Assoc. 133: 1383-1386
  3. De Rossi R, Coelho A.C., Mello G.S., et al. 2009. Effects of platelet-rich plasma gel on skin healing in surgical wound in horses. Acta Cir Bras. 24(4):276-81.
  4. Karayannopoulou M., Psalla D., Kazakos G., Loukopoulos P., Giannakas N., Savvas I., Kritsepi-Konstantinou M., Chantes A., Papazoglou L.G. Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs. Vet. Comp. Orthop. Traumatol.2015;28:172–178.
  5. Kim, H. Y., Park, J. H., Han, Y. S., Kim H. The Effect of Platelet-Rich Plasma on Flap Survival in Random Extension of an Axial Pattern Flap in Rabbuts.Plast.Reconstr. Surg. 132: 85, 2013.
  6. Kliche S, Waltenberger J. VEGF receptor signaling and endothelial function. IUBMB Life 2001;52:61–66.
  7. Li, W, Enomoto, M., Ukegawa, M., Hirai, T., Sotome, S., Wakabayashi, Y., Shinomiya, K., Okawa, A. Subcutaneous Injections of Platelet-Rich Plasma into Skin Flaps Modulate Proangiogenic Gene Expression and Improve Survival Rates. Plast.Reconstr. Surg. 129: 858, 2012.
  8. Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent 2001;10:225–8.
  9. Orhan, E, Uysal, A.C., Başer, E., Keskin, D., Yakut, C.D., The Effect Of Intradermal Administration Of Inactive Platelet Rich Plasma On Flap Viability In Rats. Acta Cir. Bras. 2017;32(3):280-286.
  10. Takikawa M., Sumi Y., Ishihara M., Kishimoto S., Nakamura S., Yanagibayashi S., Hattori H., Azuma R., Yamamoto N., Kiyosawa T. PRP&F/P MPs improved survival of dorsal paired pedicle skin flaps in rats. J. Surg. Res. 2011;170:e189–e196.
  11. Shweiki D, Itin A, Soffer D, Keshet E. Vascular endothelial growth factor induced by hypoxia may mediate hypoxia initiated angiogenesis. Nature 1992;359:843–845
  12. Wang, B., Geng, Q., Hu J., Shao, J., Ruan, J. and Zheng, J. Platelet-rich plasma reduces skin flap inflammatory cells infiltration and improves survival rates through induction of angiogenesis - An experiment in rabbits. Journal of Plastic Surgery and Hand Surgery, 2016.