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Abstract

Diabetes mellitus (DM) is one of disease that its incidence increases every year worldwide. The condition of DM can cause various complications caused by oxidative stress. Stichopus hermanii (SH) or golden sea cucumber extract contains antioxidant compounds that have been proven to reduce oxidative stress conditions. The purpose of this study was to investigate the effect of Stichopus hermanii extract on condition of diabetes mellitus by looking at changes in fasting blood glucose, plasma insulin, and malondialdehyde levels in animal models of Wistar rats. This study was a laboratory experimental study using Randomized Control Trial Design with Post-test only control group design. Thirty-five male Wistar rats divided into five groups, i.e normal control group, positive control, negative control and two treatment groups with SH extract dose 8.5 and 17 mg / kgBW for 14 days once daily after induction of Streptozotocin at the Biochemistry Laboratory of the Faculty of Medicine, Airlangga University. Fasting blood glucose level was measured by a glucometer, plasma insulin measured by ELISA and MDA level was measured by a spectrophotometer. Data were analyzed statistically by using One Way ANOVA test and Kruskal Wallis. There were significant results of SH extract can reduce fasting blood glucose (Kruskal Wallis, p=0.030) and MDA (Kruskal Wallis, p=0.042) but not in plasma insulin (ANOVA, p=0.130). The lowest MDA level occurs in the K4 group that given SH extract dose 17 mg/kg BW than another experimental group. As the conclusion, this study showed SH extract can decrease fasting blood glucose and oxidative stress in diabetic-induced rats.

Keywords

Stichopus hermanii diabetes mellitus FBG insulin MDA

Article Details

How to Cite
Prawitasari, D. S., Safitri, I., & Notopuro, H. (2019). Effects of Golden Sea Cucumber Extract (Stichopus Hermanii) on Fasting Blood Glucose, Plasma Insulin, and MDA Level of Male Rats (Rattus Norvegicus) Induced with Streptozotocin. Folia Medica Indonesiana, 55(2), 107–111. https://doi.org/10.20473/fmi.v55i2.24586

References

  1. Bajaj S, Khan A (2012). Antioxidant and diabetes. Indian Journal of Endocrinology and Metabolism 16, 267-271a
  2. Barky ARE, Ali EMM, Mohamed TM (2017). Marine sea cucumber saponins and diabetes. Austin Pancreatic Disorders 1, 1002
  3. Ceriello A, Testa R (2009). Antioxidant anti-inflammatory treatment in type 2 diabetes. Diabetes Journals care 32, S232-236
  4. Elekofehinti OO (2015). Saponins: Anti-diabetic principles from medicinal plants. A review. Elsevier, Pathophysiology 22, p 95-103
  5. Firdaus, Rimbawan, Marliyati SA, Roosita K (2016). Model tikus diabetes yang diinduksi streptozotocin-sukrosa untuk pendekatan penelitian diabetes melitus gestasional. Jurnal MKMI 12, 29-34
  6. Mu’allimah I (2017). Aktivitas antihiperglikemik sediaan teripang (Stichopus hermanii) dan spirulina (Spirulina plantesis) pada tikus putih sprague dawley yang diinduksi streptozotocin. Bogor, Institut Pertanian Bogor
  7. Olokoba AB, Obasteru OA, and Olokoba LB (2012). Type 2 diabetes mellitus: A review of current trends. Oman Medical Journal 27, 269-273
  8. Pangestuti R, Arifin Z (2017). Medicinal and health benefit effects of functional sea cucumbers. Journal of Traditional and Complementary Medicine xxx, 1-11
  9. Purwanto B, Liben P (2014). Model hewan coba untuk penelitian diabetes. Kelompok Kajian animal Model Departemen Faal FKUA. Surabaya, Revka Petra Media
  10. Revianti S, Soetjipto, Rahayu RP (2016). Protective role of Stichopus hermanii ethanol extract supplementation to oxidative stress and oral hyperkeratosis in smoking exposed rats. International Journal of ChemTech Research 9, 408-417
  11. Suryadinata RV, Wirjatmadi B, Adriani M (2017). Efektivitas penurunan malondialdehyde dengan kombinasi suplemen antioksidan superoxide dismutase melon dan gliadin akibat paparan rokok. Global Medical and Health Communicaiton 5, 79-83
  12. Veerabhadra GGK, Rahman MA, Patil S (2016). A study on antioxidants status in type 2 diabetes mellitus patients. International Journal of Biomedical and Advance Research 7, 278-280

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