Article Sidebar

Submitted
11 August 2022
Accepted
26 August 2022
Published
5 December 2022
Download
pdf
Statistic
Read Counter : 528 Download : 259

Main Article Content

Abstract

Highlights:



  1. This study demonstrated the effect of ozonated Aloe vera oil in the healing of radiation dermatitis wound.

  2. Groups that received no treatment was compared with groups that received treatment using 2.5% hydrocortisone ointment and ozonated Aloe vera.

  3. Ozonated Aloe vera oil improves the healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness.


 


Abstract :


The long-term use of corticosteroids as a standard treatment for skin disorders, such as radiation dermatitis, can cause many side effects. Alternatively, ozonated Aloe vera oil may replace corticosteroids due to its fewer side effects and benefits in wound healing process. Re-epithelialization and the formation of growth factors, such as platelet-derived growth factor (PDGF), play an important role in the healing of dermatitis wound. This study intended to demonstrate the effect of ozonated Aloe vera oil to improve the healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness. This study used a post-test only control group design. A sample of 36 Sprague-Dawley rats was divided into 6 groups (C1=without treatment, C2=2.5% hydrocortisone ointment, P1=pure Aloe vera (AV), P2=300 mg/mL ozonated Aloe vera (OAV), P3=600 mg/mL OAV, P4=1200 mg/mL OAV. The expression of PDGF was assessed using Allred scoring with immunohistochemical staining, whereas the epidermal thickness was assessed using hematoxylin and eosin (H&E) staining at 400x microscopic magnification. The PDGF expression and epidermal thickness between the control and the treatment groups showed significant differences using a Kruskal-Wallis test (P=0.001) and one-way ANOVA test (P<0.001). The groups that was given ozonated Aloe vera oil had higher average of PDGF expression and thicker epidermis than the other groups. The Spearman's correlation test showed a strong positive relationship (p<0.001 and r=0.709) between the two variables. In conclusion, ozonated Aloe vera oil improves healing of radiation dermatitis wound by increasing PDGF expression and epidermal thickness.

Keywords

Ozonated Aloe vera radiation dermatitis platelet derived growth factor (PDGF) epidermal thickness biomedics health risk increasing life expectancy

Article Details

License

Copyright (c) 2022 Folia Medica Indonesiana

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

  • Folia Medica Indonesiana is a scientific peer-reviewed article which freely available to be accessed, downloaded, and used for research purposes. Folia Medica Indonesiana (p-ISSN: 2541-1012; e-ISSN: 2528-2018) is licensed under a Creative Commons Attribution 4.0 International License. Manuscripts submitted to Folia Medica Indonesiana are published under the terms of the Creative Commons License. The terms of the license are:

    Attribution ” You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

    NonCommercial ” You may not use the material for commercial purposes.

    ShareAlike ” If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

    No additional restrictions ” You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

    You  are free to :

    Share ” copy and redistribute the material in any medium or format.

    Adapt ” remix, transform, and build upon the material.

How to Cite
Utami, W. P., Prajoko , Y. W. ., Christina H.N. Prihharsanti, Sadhana, U. ., Susilaningsih, N. ., Restiwijaya, M. ., & Nur, M. (2022). Healing of Radiation Dermatitis with Ozonated Aloe Vera Oil by Increasing PDGF and Epidermal Thickness in Sprague-Dawley Rats. Folia Medica Indonesiana, 58(4), 336–342. https://doi.org/10.20473/fmi.v58i4.38234
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Alfiaturrohmah A, Herbani M, Andriana D (2020). Efek perasan Aloe vera L. terhadap ketebalan epitel dan kepadatan kolagen pada luka sayat tikus wistar. J Bio Komplementer Med.
  2. Anzolin A, da Silveira-Kaross N, Bertol C (2020). Ozonated oil in wound healing: what has already been proven? Med Gas Res 10, 54.
  3. Atik N, Nandika A, Dewi PIC, et al (2019). Molecular mechanism of Aloe barbadensis Miller as a potential herbal medicine. Syst Rev Pharm 10, 118–25.
  4. Coondoo A, Phiske M, Verma S, et al (2014). Side-effects of topical steroids: A long overdue revisit. Indian Dermatol Online J 5, 416.
  5. Gabros S, Nessel TA, Zito PM (2022). Topical corticosteroids. StatPearls Publishing, Treasure Island, Florida.
  6. Ginting E,, Damayanti D, Fetarayani D, et al (2021). Contract dermatitis in tertiary hospital : A 2-year retrospective study. Berk Ilmu Keseh Kulit dan Kelamin 33,88.
  7. Girsang RS, Wibowo MD, Setiawan A, et al (2020). Effect of aloe vera extracts toward the fibroblast number and collegen thickness on clean skin wound healing on rattus novergicus. Syst Rev Pharm 11, 571-3.
  8. Han C, Cheng B, Wu P (2020). Clinical guideline on topical growth factors for skin wounds. Burn Trauma.
  9. Hashemi SA, Madani SA, Abediankenari S (2015). The review on properties of Aloe vera in healing of cutaneous wounds. Biomed Res Int 2015, 1–6.
  10. Heldin C, Lennartsson J, Westermark B (2018). Involvement of platelet-derived growth factor ligands and receptors in tumorigenesis. J Intern Med 283, 16–44.
  11. Hengge UR (2017). Topical corticosteroids. In Clinical and Basic Immunodermatology, pp. 815–30. Springer International Publishing, Cham.
  12. Jacobson LK, Johnson MB, Dedhia RD, et al (2017). Impaired wound healing after radiation therapy: A systematic review of pathogenesis and treatment. JPRAS Open 13, 92–105.
  13. Kim HS, Noh SU, Han YW, et al (2009). Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci 24, 368.
  14. Kumar R, Singh AK, Gupta A, et al (2019). Therapeutic potential of Aloe vera”A miracle gift of nature. Phytomedicine 60, 152996.
  15. National Institutes of Health-National Cancer Institute (2017). Common Terminology Criteria for Adverse Events (CTCAE) version 5.0: U.S. Department of Health and Human Services, National Institutes of Health, National Cancer Institute.
  16. Paramata NR, Abas NA (2019). Pengaruh kompres Aloe vera pada penderita dermatitis di wilayah kerja Puskesmas Sumalata Kabupaten Gorontalo Utara. Jambura Nurs J 1, 19–23.
  17. Ran X, Cheng T, Shi C, et al (2004). The effects of total-body irradiation on the survival and skin wound healing of rats with combined radiation-wound injury. J Trauma Inj Infect Crit Care 57, 1087–93.
  18. Rao S, Hegde S, Baliga-Rao M, et al (2017). An Aloe vera-based cosmeceutical cream delays and mitigates ionizing radiation-induced dermatitis in head and neck cancer patients undergoing curative radiotherapy: A clinical study. Medicines 4, 44.
  19. Robijns J, Laubach H-J (2018). Acute and chronic radiodermatitis. J Egypt Womenʼs Dermatologic Soc 15, 2–9.
  20. Rosenthal A, Israilevich R, Moy R (2019). Management of acute radiation dermatitis: A review of the literature and proposal for treatment algorithm. J Am Acad Dermatol 81, 558–67.
  21. Singh M, Alavi A, Wong R, et al (2016). Radiodermatitis: A review of our current understanding. Am J Clin Dermatol 17, 277–92.
  22. SpaÅ‚ek M (2016). Chronic radiation-induced dermatitis: Challenges and solutions. Clin Cosmet Investig Dermatol Volume 9, 473–82.
  23. Tamba US, Arifin MT, Nur M, et al (2020). The role of ozonated Aloe vera oil in full-thickness skin defects: Macrophage count and epithelization length parameter. F1000Research 9, 1218.
  24. Vadarli G, Angelo-Khattar M (2020). A fibroblast-derived human growth factor preparation for the management of acute radiodermatitis: A case report. Int Med Case Rep J Volume 13, 691–6.
  25. Vahlepi R, Arifin MT, Nur M, et al (2020). Ozonated aloe vera oil improve wound healing by decreasing neutrophil count and wound diameter in Sprague dawley rats with full-thickness skin defect. Indones J Biomed Sci 14, 48.
  26. Valacchi G, Lim Y, Belmonte G, et al (2011). Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice. Wound Repair Regen 19, 107–15.
  27. Wang Y, Tu W, Tang Y, et al (2020). Prevention and treatment for radiation-induced skin injury during radiotherapy. Radiat Med Prot 1, 60–8.
  28. Williams DM (2018). Clinical pharmacology of corticosteroids. Respir Care 63, 655–70.
  29. Zeng J, Dou J, Gao L, et al (2020). Topical ozone therapy restores microbiome diversity in atopic dermatitis. Int Immunopharmacol 80, 106191.