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Submitted
8 March 2023
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17 November 2023
Published
10 September 2023
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Abstract

Highlights:


1.Due to the adverse effects associated with current antifungal drugs, research on traditional medicine is necessary to explore other options for candidiasis treatment.
2.Following the findings of this study, it is recommended to conduct further research by incorporating Coleus scuttellarioides filtrate into the growth medium, since this may improve bacterial growth by producing optimal secondary metabolites.


 


Abstract


Candida albicans is the most common organism responsible for both mucosal and systemic infections, accounting for approximately 70% of fungal infections worldwide. Miana, scientifically known as Coleus scuttellarioides (L.) Benth., is recognized for its use in traditional medicinal practices. Miana plants contain endophytic bacteria that possess the ability to produce secondary metabolites with potential antifungal agents. The objective of this study was to assess the antifungal activity of nine endophytic bacteria isolates derived from Coleus scuttellarioides against Candida albicans. This study was laboratory-based qualitative experimental research that applied the Kirby-Bauer diffusion method and several modifications. The Candida albicans specimens were spread throughout the entire potato dextrose agar medium. Afterwards, paper discs that had been soaked in a liquid culture of endophytic bacterial isolates were carefully placed on the surface of the medium. The complete setup was then incubated for 1–2 days. The potential antifungal activity of endophytic bacteria was assessed by observing the emergence of a clear zone surrounding their growth, which would indicate inhibition. An additional observation was performed in the follow-up test, involving the use of Sabouraud dextrose agar medium to confirm the initial test result. The results from the inhibitory test revealed that none of the bacterial isolates exhibited any inhibition zone. Conversely, ketoconazole as the positive control showed an inhibition zone with an average diameter of 28.5 mm. In conclusion, endophytic bacterial isolates obtained from Coleus scuttellarioides have no discernible antifungal properties against Candida albicans. This study implies that ketoconazole remains effective in treating infections caused by Candida albicans.

Keywords

Candida albicans Coleus scutellarioides (L.) Benth endophytic bacteria human and health tropical disease

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How to Cite
Melda Yunita, Ruth Magdalena Lumbantobing, & Ritha Tahitu. (2023). Antifungal Activity of Endophytic Bacteria Isolated from Miana Plants (Coleus scutellarioides (L.) Benth.) against Candida albicans. Folia Medica Indonesiana, 59(3), 274–281. https://doi.org/10.20473/fmi.v59i3.43989
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References

  1. Alioes Y, Kartika A (2019). Uji potensi antijamur Candida albicans ekstrak daun gelinggang (Cassia alata L.) dibandingkan dengan sediaan saun sirih yang beredar di pasaran secara in vitro. Jurnal Kimia Riset 3, 108. doi: 10.20473/jkr.v3i2.12040.
  2. Aprianti I, Maulana IT, Syafnir L (2019). Telaah golongan senyawa antibakteri Shigella dysenteriae dari ekstrak bertingkat alga merah (Eucheuma spinosum) menggunakan metode KLT bioautografi. Prosiding Farmasi. doi: http://dx.doi. org/10.29313/.v0i0.18144.
  3. Bhateja S (2018). Candidiasis- The most common fungal infection of oral cavity. Biomedical Journal of Scientific & Technical Research. doi: 10.26717/ BJSTR.2018.08.001649.
  4. Fanning S, Mitchell AP (2012). Fungal biofilms ed. Heitman J. PLoS Pathogens 8, e1002585. doi: 10.1371/journal.ppat.1002585.
  5. Foeh S, Temaj I, Khallimi K (2019). Potensi bakteri endofit dalam menekan pertumbuhan Phytophthora palmivora (Butler) secara in vitro. J Agroekoteknologi Trop (Journal Trop Agroecotechnology). Available at: https://ojs.unud.ac.id/index.php/JAT/article/view/54480.
  6. Indrayati S, Sari RI (2018). Gambaran Candida albicans pada bak penampung air di toilet SDN 17 Batu Banyak Kabupaten Solok. Jurnal Kesehatan Perintis (Perintis's Heal Journal) 5, 133–138. doi: 10.33653/jkp.v5i2.148.
  7. Jamilatun M, Aminah A, Shufiyani S (2020). Uji daya hambat antibakteri kapang endofit dari tanaman alang-alang (Imperata cylindrica (L.) Beauv.) terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Medikes (Media Informasi Kesehatan) 7, 335–346. doi: 10.36743/medikes.v7i2.224.
  8. Kalsum U, Ayu A (2019). Uji aktivitas ekstrak etanol umbi wortel (Daucus carota L.) sebagai antifungi terhadap pertumbuhan Candida albicans. Warta Farmasi 8, 71–80. doi: 10.46356/wfarmasi.v8i2.117.
  9. Levita J, Sumiw SA, Pratiwi TI, et al (2016). Pharmacological activities of Plectranthus scutellarioides (L.) R.Br. leaves extract on Cyclooxygenase and Xanthine oxidase enzymes. Journal of Medicinal Plants Research 10, 261–269. doi: 10.5897/JMPR2016.6105.
  10. Lieberman A, Curtis L (2018). Severe adverse reactions following ketoconazole, fluconazole, and environmental exposures: A case report. Drug Safety - Case Reports 5, 18. doi: 10.1007/s40800-018-0083-2.
  11. Mahulette J (2022). Potensi bakteri endofit yang diisolasi dari tanaman miana (Coleus scutellaroides (L) Benth) dalam menghambat pertumbuhan bakteri Salmonella typhi (thesis). Universitas Pattimura.
  12. Makhfirah N, Fatimatuzzahra C, Mardina V, et al (2020). Pemanfaatan bahan alami sebagai upaya penghambat Candida albicans pada rongga mulu. Jurnal Jeumpa 7, 400–413. doi: 10.33059/ jj.v7i2.3005.
  13. Marpaung PNS (2014). Uji efektivitas sediaan salep ekstrak daun miana (Coleus scutellarioides [L] Benth.) untuk pengobatan luka yang terinfeksi bakteri Staphylococcus aureus pada kelinci (Oryctolagus cuniculus). Pharmacon. doi: 10.35799/pha.3.2014.5360.
  14. Maulana HR, Sumardi U, Koesoemadinata RC (2019). Prevalensi kandidiasis invasif di unit perawatan intensif Rumah Sakit Hasan Sadikin Bandung. Jurnal Penyakit Dalam Indonesia 6, 8. doi: 10.7454/jpdi.v6i1.276.
  15. Maulana RN, Zulfa F, Setyaningsih Y (2020). Uji efektivitas ekstrak kulit pisang ambon (Musa paradisiaca var. Sapientum L.) terhadap pertumbuhan Trichophyton rubrum secara In Vitro. Pros Semin Nas Ris Kedokt (Sensorik 2022). Available at: https://conference.upnvj.ac.id /index.php/sensorik/article/view/426.
  16. Muljono P, Fatimawali, Manampiring AE (2016). Uji aktivitas antibakteri ekstrak daun mayana jantan (Coleus atropurpureus Benth) terhadap pertumbuhan bakteri Streptococcus Sp. dan Pseudomonas Sp. eBiomedik. doi: 10.35790/ebm.v4i1.10860.
  17. Novanti H, Susilawati Y (2017). Aktivitas farmakologi daun iler (Plectranthus scutellarioides (L.) R.Br.). Farmaka. doi: 10.24198/jf.v15i1. 13196.
  18. Nursalwa N, Karim H, Ali A (2018). Isolasi bakteri endofit pada tanaman family Zingeberaceae dan uji aktivitas antimikroba. Prosiding Seminar Nasional Biologi dan Pembelajarannya. Available at: https://ojs.unm.ac.id/semnasbio/article/view/6967.
  19. Pierce CG, Srinivasan A, Uppuluri P, et al (2013). Antifungal therapy with an emphasis on biofilms. Current Opinion in Pharmacology 13, 726–730. doi: 10.1016/j.coph.2013.08.008.
  20. Puspitasari A, Kawilarang AP, Ervianty E, et al (2019). Profil pasien baru kandidiasis. Berkala Ilmu Kesehatan Kulit dan Kelamin 31, 24034. doi: 10.20473/bikk.V31.1.2019.24-34.
  21. Rachman EA, Sari SR (2020). Uji daya hambat isolat bakteri endofit tanaman kayu jawa Lannea coromandelica (Houtt.) Merr. terhadap Candida albicans. Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan 11, 8. doi: 10.32502/sm.v11i1.2140.
  22. Romo JA, Kumamoto CA (2020). On commensalism of Candida. Journal of Fungi 6, 16. doi: 10.3390/jof6010016.
  23. Rubio RG, de Oliveira HC, Rivera J, et al (2020). The fungal cell wall: Candida, cryptococcus, and aspergillus species. Frontiers in Microbiology. doi: 10.3389/fmicb.2019.02993.
  24. Setianingrum DA, Bintang M, Syaefudin (2014). Aktivitas antifungi ekstrak daun miana (Coleus scutellariodes [L] benth) pada pertumbuhan Candida albicans secara in vitro. Scientific Repository. Available at: http://repository.ipb.ac.id/handle/123456789/70389.
  25. She X, Calderone R, Kruppa M, et al. (2016). Cell wall N-linked mannoprotein biosynthesis requires goa1p, a putative regulator of mitochondrial complex I in Candida albicans ed. Chauhan N. PLoS One 11, e0147175. doi: 10.1371/journal. pone.0147175.
  26. Sinawe H, Casadesus D (2023). Ketoconazole. Available at: http://www.ncbi.nlm.nih.gov/pub med/0.
  27. Sulistiyani S, Ardyati T, Winarsih S (2016). Antimicrobial and antioxidant activity of endophyte bacteria associated with Curcuma longa rhizome. The Journal of Experimental Life Sciences 6, 45–51. doi: 10.21776/ub.jels.2016. 006.01.11.
  28. Sulistrioningsih S, Wardoyo ERP, Kurniatuhadi R (2020). Aktivitas antifungi ekstrak metanol daun salam (Syzygium polyanthum [Wight] Walp.) terhadap pertumbuhan Malassezia sp. (M1) secara in vitro. Protobiont: Journal of Biological Sciences. doi: 10.26418/protobiont.v9i2.45849.
  29. Sulmiyati S, Said NS, Fahrodi DU, et al (2019). The characteristics yeast isolated from commercial kefir grain, Indonesia. Hasanuddin Journal of Animal Science (HAJAS) 1, 26–36. doi: 10.20956/hajas.v1i1.6519.
  30. Talapko J, JuzbaÅ¡ić M, Matijević T, et al (2021). Candida albicans”The virulence factors and clinical manifestations of infection. Journal of Fungi 7, 79. doi: 10.3390/jof7020079.
  31. Tidore M (3AD). Pengaruh tempat dan interval waktu konseling terhadap kepuasaan layanan konseling pada pelayanan kesehatan peduli remaja di Puskesmas Kota Ambon. 2018. doi: 10.33846/ghs.v3i4.308.
  32. Vinayarani G, Prakash HS (2018). Growth promoting rhizospheric and endophytic bacteria from Curcuma longa L. as biocontrol agents against rhizome rot and leaf blight diseases. The Plant Pathology Journal 34, 218–235. doi: 10.5423/PPJ.OA.11.2017.0225.
  33. Wakhidah AZ, Silalahi M (2018). Etnofarmakologi tumbuhan miana (Coleus scutellariodes (L.) Benth) pada masyarakat Halmahera Barat, Maluku Utara. Pro-Life. Available at: http://ejournal.uki. ac.id/index.php/prolife/article/view/711.
  34. Winastri NLAP, Muliasari H, Hidayati E (2020). Aktivitas antibakteri air perasan dan rebusan daun calincing (Oxalis corniculata L.) terhadap Streptococcus mutans. Berita Biologi. doi: 10.14203/beritabiologi.v19i2.3786.
  35. Wu W, Chen W, Liu S, et al (2021). Beneficial relationships between endophytic bacteria and medicinal plants. Frontiers in Plant Science. doi: 10.3389/fpls.2021.646146.
  36. Yunita M, Manse Y, Wulandari MC, et al (2022). Isolation of endophytic bacteria from nutmeg plant as antibacterial agents against pathogenic bacteria. JPBIO (Jurnal Pendidikan Biologi) l 7, 115–122. doi: 10.31932/jpbio.v7i1.1522.
  37. Yunita M, Mubarik NR, Solihin DD (2016). Isolation and identification of chitinolytic bacteria as biocontrol agent of pathogenic fungi on gold silkworm cocoon cricula trifenestrata. Malaysian Journal of Microbiology. doi: 10.21161/mjm.74415.
  38. Yunita M, Ohiwal M, Dirks CS, et al (2022). Endophytic bacteria associated with Myristica fragrans Houtt: Improved media, bacterial population, preliminary characterization, and potential as antibacterials. Biodiversitas Journal of Biological Diversity. doi: 10.13057/biodiv/d23 0824.
  39. Zuraidah Z, Gunawan A, Agustina E (2021). Uji daya hambat ekstrak daun sirih hijau (Piper betle L.), daun sirih merah (Piper crocatum Ruiz & Pav.), dan daun sirih hutan (Piper aduncum L.) terhadap pertumbuhan Candida albicans. Jurnal Ilmu Alam dan Lingkungan. doi: 10.20956/jal.v12i2.17587.