THE EFFECT OF SOURSOP LEAF EXTRACT ON PANCREATIC BETA CELL COUNT AND FASTING BLOOD GLUCOSE IN MALE WISTAR RATS EXPOSED TO A HIGH-FAT DIET AND STREPTOZOTOCIN

Dewa Ayu Agung Alit Suka Astini, H Ari Gunawan, R Mochamad Wirono Aman Santoso, Susilowati Andajani, Ahmad Basori

= http://dx.doi.org/10.20473/fmi.v53i1.5484
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Abstract


Based on some researches known that soursop leaf extract can improve beta cell injury. The aims of this study was to analyze the effect of soursop leaf extract on fasting blood glucose (FBG) and pancreatic beta cell number in male Wistar rats wich were exposed to a high-fat diet and streptozotocin. This study design is the only randomized posttest control group design. The total sample size is 50 male Wistar rats. The independent variable: high-fat diet, STZ, and soursop leaf extract; the dependent variable: pancreatic beta cells number, and FBG3. Data tested for normality with Kolmogorov-Smirnov (a=0.05) and tested of homogeneity with Levene (a =0.05). Comparison test between groups with Kruskal-Wallis (a=0.05), followed by Mann Whitney. Correlation test with Pearson (a=0.05) between dose of the soursop leaf extract and FBG3, and between dose and the number of pancreatic beta cells. The results of this study showed that the soursop leaf extract at a dose of 100 mg/kg and 150 mg/kg have an effect on fasting blood glucose levels and panreatic beta cells number;2)There is a significant negative correlation between the orograstric lavage of soursop leaf extract with FBG3 (r=-0.647;p<0.001), the increasing doses of soursop leaf extract, further lowering fasting blood glucose levels;3)There is a significant positive correlation between the orograstric lavage of soursop leaf extract with the number of pancreatic beta cells (r=0,759;p<0,001), the increasing doses of soursop leaf extract, further increasing pancreatic beta cells number. In conclusion, increasing doses of soursop leaf extract, further lowering fasting blood glucose and increasing the number of pancreatic beta cells.

Keywords


high-fat diet; STZ; soursop leaf extract; FBG; pancreatic beta cells

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References


Adewole, SO, Ojewole, AO 2009, ‘Protective Effects Of Annona muricata Linn. (Annonaceae) Leaf Aqueous Extract On Serum Lipid Profiles And Oxidative Stress In Hepatocytes of Streptozotocin-Treated Diabetic Rats’, Afr. J. Trad. CAM, vol 6, no. 1, pp. 30 – 41.

Adeyemi, DO, Komolafe, OA, Adewole, OS, Obuotor, EM, Abiodun, AA, Adenowo, TK 2010, ‘Histo-morphological and morphometric studies of the pancreatic islet cells of diabetic rats treated with extract of Annona muricata’, Via Medica,vol. 69, no. 2, pp. 92-100.

Chen, L, Magliano, DJ, Zimmet, PZ 2012, ‘The worldwide epidemiology of type 2 diabetes mellitus-present and future perspectives’, Endocrinol, vol. 8, pp. 228-236.

Day, C, Bailey, CJ 2011, ‘Obesity in the pathogenesis of type 2 diabetes’ The British Journal of Diabetes & Vascular Disease, vol. 11, no. 55, pp. 54-61.

Hosseini, A, Shafiee-Nick, R, Ghorbani, A 2015, ‘Pancreatic beta cell protection/regeneration with phytotherapy’, Brazilian Journal of Pharmaceutical Sciences, vol.51, no. 1, pp. 1-16.

Kedari, TS, Khan, AA 2014, ‘Guyabano (Annona Muricata): A review of its Traditional uses Phyto-chemistry and Pharmacology’, American Journal of Research Communication, vol. 2, no. 10, pp. 247-22.

Kumar, V, Abbas, AK, Fausto, N, Aster, JC 2010, ‘The Endocine Pancreas’, in Robbin and Cotran Pathologic Basis of Disease (8th ed), Saunders,an imprint of Elsevier Inc, Philadelphia, pp. 1130-1148.

Malviya, N, Jain, S, Malviya S 2010, ‘Antidiabetic Potential of Medicinal Plants’, Acta Poloniae Pharma-ceutica-Drug Research, vol. 67, no. 2, pp. 113-118.

Muthu & Duraira 2015, ‘Evaluation of antioxidant and free radical scavenging activity of Annona muricata’, European Journal of Experimental Biology, vol. 5, no. 3, pp. 39-45.

Pinent, M, Castell, A, Baiges, I, Montagut, G, Arola, L, Ard´evol, A 2008, ‘Bioactivity of Flavonoids on Insulin-Secreting Cells’, Comprehensive Reviews in Food Science and Food Safety, vol. 7, pp. 299-308.

Ramachandran, A, Snehalatha, C, Shetty, AS, Nanditha, A 2012, ‘Trends in prevalence of diabetes in Asian countries’, World Journal of Diabetes, vol. 3, no. 6, pp. 110-117.

Rimbun 2015, ‘Tesis Profil Glukosa Darah Luas Pulau Langerhans dan Ekspresi Reseptor Insulin Otot pada Berbagai Model Diabetes Mellitus Tipe 2’, Program Studi Kedokteran Dasar Jenjang Magister Fakultas Kedokteran Universitas Airlangga, Surabaya.

Saisho, Y 2014, ‘Importance of Beta Cell Function for the Treatment of Type 2 Diabetes’, J. Clin. Med, vol. 3, pp. 923-943.

Scottish Intercollegiate Guidelines Network 2010, ‘Ma-nagement of diabetes’.

Shaw, JE, Sicree, RA, Zimmet, PZ 2010, ‘Global estimates of the prevalence of diabetes for 2010 and 2030’, Diabetes Research and Clinical Practice, vol. 87, pp. 4-14.

Soewondo, P, Ferrario, A, Tahapary L 2013, ‘Challeng-es in diabetes management in Indonesia: a literature review’, Globalization and Health, vol. 9, no. 63, pp. 1-17.

Srinivasan, K, Ramarao, P 2007, ‘Animal models in type 2 diabetes research: An overview’, Indian J Med Res, vol. 125, pp. 451-472.


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