Article Sidebar

Submitted
30 October 2017
Accepted
30 October 2017
Published
3 November 2017
Download
PDF
Statistic
Read Counter : 951 Download : 1430

Main Article Content

Abstract

The objects contained in crime scene and its surroundings have significance in the examination of forensic identification. The most commonly used specimens in the examination for identification are blood/blood spots, semen patches, vaginal swabs, buccal swabs and bones, including items often used by the perpetrator/victim the last time. For example, a mobile phone hearing aid (headset/earphone). In the use of earphones attached to the outer ear skin so it is suspected there is a serumen attached to the tool. One factor that can affect the quality of DNA is the prolonged exposure. Until now in Indonesia the effect of long exposure to room temperature on the quality of DNA on the DNA material of earphone swabs through DNA analysis has not been widely known. The type of study was laboratory experimental. Used earphones are exposed at room temperature within 1, 7, 14 and 20 days. The results of this study indicate that the environmental effect, ie the duration of exposure, affects the decrease in DNA content significantly from day 1 to 20. Detection of PCR mtDNA D-Loop HVS I visualization results 143 bp nt: 16268 16410 shows positive detection results (+) Only at day 1 exposure to room temperature [4 sample/66,67%] and day 7 [3 sample/50%]. Visualization of PCR results mtDNA D-Loop HVS II 126bp nt: 34 -159 was performed on day 1 day room exposure [2 sample/33,37%] and day 7 [6 sample/100%]. Conclusion, the duration of exposure to room temperature affect the quality of DNA from earpiece swab material. Decreased levels of earphones DNA swabs showed a significance value (p <0.005) against the effect of long time exposure to room temperature.

Keywords

earphone swab DNA quality mitochondrial DNA

Article Details

License

  • Folia Medica Indonesiana is a scientific peer-reviewed article which freely available to be accessed, downloaded, and used for research purposes. Folia Medica Indonesiana (p-ISSN: 2541-1012; e-ISSN: 2528-2018) is licensed under a Creative Commons Attribution 4.0 International License. Manuscripts submitted to Folia Medica Indonesiana are published under the terms of the Creative Commons License. The terms of the license are:

    Attribution ” You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

    NonCommercial ” You may not use the material for commercial purposes.

    ShareAlike ” If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.

    No additional restrictions ” You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

    You  are free to :

    Share ” copy and redistribute the material in any medium or format.

    Adapt ” remix, transform, and build upon the material.

How to Cite
Yudianto, A., & Margaret, N. (2017). EFFECT OF ROOM TEMPERATURE ON THE QUALITY OF DNA FROM EARPHONE SWAB BY OBSERVING MITOCHONDRIAL DNA [mtDNA] D-LOOP REGION OF 126 bp (HVS II, nt 34-159) AND 143 bp (HVS I, nt 16268-16410). Folia Medica Indonesiana, 53(2), 86–93. https://doi.org/10.20473/fmi.v53i2.6342
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Bartlett JM, Stirling D (2003). A short history of the polymerase chain reaction. Methods Mol Biol 226, 3-6
  2. Butler JM, Shen Y, McCord BR (2003). The develop-ment of reduced size STR amplicons as tools for analysis of degraded DNA. J Forensic Sc 48, 1054-64
  3. Edson SM, Ross JP, Coble MD, et al (2004). Naming the dead-confronting realities of rapid identification of degraded skeletal remains:Forensic Science Rev Vol 16, 69-74
  4. Gabriel MN, Huffine EF, Ryan JH, et al (2001). Improved mtDNA sequence analysis of forensic remains using a "mini-primer” set amplification strategy; J Forensic Sci 46, 247
  5. Kusuma SE, Sosiawan A (2004). Efek temperature ekstrim pada DNA inti dan DNA mitokondria. Preliminary Research, Research Institution of Airlangga University.
  6. Muladno (2002). Seputar teknologi rekayasa genetika. 1st ed. Bogor, Pustaka Wirausaha Muda
  7. Notosoehardjo I (1999). Penentuan Jenis Kelamin berdasarkan Pemeriksaan DNA dan Antropometri tulang. Doctoral Dissertation, Airlangga University, Surabaya.|
  8. Seo Y, Uchiyama T, Matsuda H, et al (2002). Mitocho-ndrial DNA and STR typing of matter adhering to an earphone. Journal of Forensic Sci 47, 605-608
  9. Yudianto A (2009). Sex determinant melalui pemeriksa-an DNA rambut manusia. Medical research unit, Airlangga University, Surabaya
  10. Yudianto A (2010). Analisis DNA tulang dan gigi pada lokus short tandem repeat-combined DNA index system (STR-CODIS),Y-Chromosome STRs & mito-chondrial DNA (mtDNA) akibat efek paparan panas suhu tinggi. Dissertation, Airlangga University, Surabaya
  11. Yudianto A, Sispitasari YE, Margaret N (2015). Analis-is DNA mitokondria swab earphone sebagai bahan alternative pemeriksaan identifikasi forensic. Penelitian Unggulan Sekolah Pascsarjana, Airlangga University, Surabaya
  12. Yuwono T (2006). Teori dan aplikasi polymerase chain reaction. Yogyakarta, Penerbit Andi

Most read articles by the same author(s)