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Submitted
4 November 2017
Accepted
4 November 2017
Published
4 November 2017
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Abstract

Eight isolates of Streptomyces sp. can be isolated from compost soil in Surabaya, Indonesia. The results of biochemical and morphological tests showed that the 8 isolates were new. Phylogenetic analysis was performed on the sequence of 16S rRNA gene. Nucleotide sequences of 16S rRNA gene Streptomyces sp. the compost soil isolates of Surabaya were analyzed and compared with the 16S rRNA gene sequence from the literature. Streptomyces sp. the compost soil of Surabaya based on the 16S rRNA gene showed the new species of Streptomyces. The result of phylogenetic tree diagram showed that Streptomyces Sp-D, Sp-Ep, Sp-G and Sp-I found in Bratang Surabaya compost house land were new isolates. Streptomyces Sp-Ep was a new type of Streptomyces closely related to Streptomyces indonesiasis and Streptomyces nashvillensis. Streptomyces Sp-Ea was Streptomyces olivoreticuli which was still related to Streptomyces yogyakartensis. Streptomyces Sp-F was Streptomyces levis strain NRRL B-24299. Streptomyces Sp-C was Streptomyces filamentosus. Streptomyces Sp-D was a new type of Streptomyces closely related to Streptomyces javensis and Streptomyces roseus. Streptomyces Sp-G was a new type of Streptomyces closely related to Streptomyces roseoviridis strain NBRC 12911 and Streptomyces thermocarboxydovorans strain AT52. Streptomyces Sp-I was a new streptomyces that was still closely related to Streptomyces cangkringensis and Streptomyces asiaticus. Streptomyces Sp-A was Streptomyces laurentii strain: LMG 19959.

Keywords

Phylogenetic analysis antimicrobial streptomyces compost soil 16S rRNA

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  • Folia Medica Indonesiana is a scientific peer-reviewed article which freely available to be accessed, downloaded, and used for research purposes. Folia Medica Indonesiana (p-ISSN: 2541-1012; e-ISSN: 2528-2018) is licensed under a Creative Commons Attribution 4.0 International License. Manuscripts submitted to Folia Medica Indonesiana are published under the terms of the Creative Commons License. The terms of the license are:

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How to Cite
Kurnijasanti, R., Isnaeni, I., AT, P., & Sudjarwo, S. (2017). PHYLOGENETIC ANALYSIS AND ANTI MICROBIAL ACTIVITY OF Streptomyces spp. ISOLATED FROM COMPOST SOIL IN SURABAYA INDONESIA ON THE BASIS OF 16S Rrna GENE. Folia Medica Indonesiana, 53(3), 204–208. https://doi.org/10.20473/fmi.v53i3.6456
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References

  1. Barakate M, Ouhdouch Y, Oufdou K, Beaulieu C (2002). Characterization of rhizospheric soil strepto-mycetes from Moroccan habitats and their antimi-crobial activities. World J. Microbiol. Biotechnol 17, 49-54
  2. Champness W (2000). Actinomycete development, antibiotic production and phylogeny: questions and challenges. In: Brun YV and Skimkets LJ (ed.). Prokaryotic Development. Washington D.C, The American Society for Microbiology, p 11-31
  3. Corbin DR, Grebenok RJ, Ohnmeiss TE, Greenplate JT, Purcell JP (2001). Expression and chloroplast targeting of cholesterol oxidase in transgenic tobacco plants. Plant Physiol 126, 1116-1128
  4. Davelos AL, Xiao K, Samac DA, Kinkel LL (2004). Spatial variation in the frequency and intensity of antibiotic interaction among streptomycetes in a prairie soil. Appl Environ Microbiol 70, 1051-1058
  5. Dhanasekaran D, Rajakumar G, Sivamani P, Selvamani S, Panneerselvam A, Thajuddin N (2005). Screening of salt pans actinomycetes for antibacterial agents. Int. J. Microbiol 1, 6-12
  6. Iznaga Y, Lemus M, Gonzalez L, Garmendia L, Nadal L, Vallin C (2004). Antifungal activity of actinomy-cetes from Cuban soils. Phytother. Res. 18, 494-496
  7. Kim SB, Goodfellow M (2002). Streptomyces avermi-tilis sp. nov., nom. rev., a taxonomic home for the avermectin-producing streptomycetes. Int J Syst Evol Microbiol 52, 2011–2014
  8. Kim SB, Seong CN, Jeon SJ, Bae KS, Goodfellow M (2004). Taxonomic study of neutrotolerant acidophilic actinomycetes isolated from soil and description of Streptomyces yeochonensis sp. nov. Int J Syst Evol Microbiol 54, 211–214
  9. Saintpierre D, Amir H, Pineau R, Sembiring L, Good-fellow M (2003). Streptomyces yatensis sp. nov., a novel bioactive streptomycete isolated from a New-Caledonian ultramafic soil. Antonie van Leeuwenhoek 83, 21–26.
  10. Shayne JJ, Hugenholtz P, Sangwan P, Osborne C, Janssen HP (2003). Laboratory cultivation of wide-spread and previously uncultured soil bacteria. App. Environ. Microbiol 69, 7211-7214
  11. Tamura K, Peterson D, Peterson N, et al (2011). MEGA 5: Molecular evolutionary distance and maxi-mum parsimony methods. Mol Biol Evol 28, 2731-2739