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Intra Uterine Fetal Death (IUFD) is one contributor to infant mortality. Human Leukocyte Antigen-E (HLA-E) and Natural Killer Cells (NK cells) are believed to play an important role towards IUFD associated with immune maladaptation. This study aimed to determine the expression of HLA-E and NK cells on trophoblast on IUFD and normal pregnancy. The study used cross sectional approach undertaken at Department of Obstetrics and Gynecology, Dr. Moewardi Hospital, Surakarta, networking hospitals, and Anatomic Pathology Laboratory of Faculty of Medicine, Sebelas Maret University. The number of samples were 32 subjects consisting of 16 subjects with normal pregnancy and 16 subjects with IUFD. The expression of HLA-E and NK cells on trophoblast of subjects who met the inclusion and exclusion criteria were examined using immunohistochemistry method and t-test statistical analysis. The mean value of HLA-E expression in the trophoblast of IUFD group was 17.30±6.69, in normal pregnancy was 57.06±32.04, with p=0.00 (p<0.05). The mean value of NK cell expression in trophoblast in IUFD group was 78.62±36.43, in normal pregnancy was 19.87±6.43, with p=0.00 (p<0.05). This study concluded that the expression of HLA-E was lower and NK cells wass higher in IUFD compared to those in normal pregnancy.


HLA-E NK cells Intra Uterine Fetal Death

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How to Cite
Sulistyowati, S., Nugroho, M. A., Respati, S. H., & Soetrisno, S. (2017). EXPRESSION OF HUMAN LEUKOCYTE ANTIGEN-E AND NATURAL KILLER CELLS IN INTRA UTERINE FETAL DEATH. Folia Medica Indonesiana, 53(4), 252–257.


  1. Al-Kadri HM, Tamim HM (2012). Factors contributing to intra-uterine fetal death. Arch Gynecol Obstet 286, 1109-16
  2. Flenady V, Koopmans L, Middleton P, Froen JF, Smith GC, Gibbons K et al (2011). Major risk factors for stillbirth in high-income countries: a systematic review and meta-analysis. The Lancet 377, 1331–1340
  3. Fotoohi M, Ghasemi N, Mirghanizadeh SA, Vakili M, Samadi M (2016). Association between HLA-E gene polymorphism and unexplained recurrent spontaneous abortion (RSA) in Iranian women. Int J Reprod Biomed (Yazd) 14, 477 – 482
  4. Fukui A, Yokota M, Funamizu A, Nakamua R, Fukuhara R, Yamada K et al (2012). Changes of NK cell in preeclampsia. Am J Reprod Immunol 67, 278-86
  5. Gerungan EN, Pascoal M, Lontaan A (2016). Faktor-faktor yang berhubungan dengan kejadian intra uterine fetal death (IUFD). Jurnal Ilmiah Bidan 4, 9 – 14
  6. Haumonte JB, Caillat-Zucman S, Bretelle F, Lambert M, Lyonnet L, Levy-Mozziconacci A, et al (2014). Enhanced prevalence of plasmatic soluble MHC class i chain-related molecule in vascular pregnancy diseases. BioMed Research International 2014, 1 – 11
  7. Hunt JS, Langat DL (2009). HLA-G: A human pregnancy-related immunomodulator. Curr Opin Pharmacol, 9, 462–469
  8. Hviid TVF (2006). HLA-G in human reproduction: aspects of genetics, function and pregnancy complica-tions. Human Reproduction Update 12, 209–232
  9. Mallia JV, Das DK, Maitra A (2012). Role of HLA in pregnancy. Int J Hum Genet 12(1), 33 – 6
  10. Matter TF, Sharif FA (2013). HLA-G and HLA-E Gene polymorphisms in idiopathic recurrent spontaneous abortion women in Gaza strip-Palestine. Int J Reprod Contracept Obstet Gynecol 2,3,277-283
  11. Mattingly PJ, Joy S, Talavera F (2016). Evaluation of fetal death. Medscape. /article/259165-overview
  12. Morandi F, Airoldi I, Pistoia V (2014). IL-27 Driven Upregulation of Surface HLA-E Expression on Monocytes Inhibits IFN- Release by Autologous NK Cells. Journal of Immunology Research 2014.
  13. Murti B (2010). Desain dan Ukuran Sampel Untuk Penelitian kuantitatif dan Kualitatif di Bidang Kesehatan. Yogyakarta: universitas gajah Mada.
  14. Patel S, Thaker R, Shah P, Majumder S (2014). Study of causes and complications of intra uterine fetal death (IUFD). Int J Reprod Contracept Obstet Gynecol 3(4), 931-935
  15. Rodrigues MN, Favaron PO, Dombrowski JG, Souza RM, Miglino MA (2013). Role of natural killer (NK) cells during pregnancy: A review. Open Journal of Animal Sciences 3, 138-144
  16. Sargent IL, Borzychowski AM, Redman CWG (2007). NK Cell and pre-eclampsia. J Reprod Immunol 76(1-2),40 – 4
  17. Sharma S (2014). Natural killer cells and regulatory T cells in early pregnancy loss. Int J Dev Biol 58, 0, 219–229.
  18. Silver RM (2007). Fetal death. Obstet Gynecol 109,153–67
  19. Sulistyowati S, Abadi A, Hood J, Soetjipto (2010). The influence of low HLA-G protein expression on Hsp-70 and VCAM-1 profile in preeclampsia. Indones J Obstet Gynecol 34(4), 185 – 190.
  20. Tripathi P, Naik S, Agrawal S (2007). Role of HLA-G, HLA-E and KIR2DL4 in Pregnancy. Int J Hum Genet 7, 219-233
  21. Yuristin D (2014). Hubungan anemia dengan kejadian Intra Uterine Fetal Death (IUFD) di RSUD Arifin Achmad Pekanbaru Propinsi Riau tahun 2011 – 2012. ISSN 97724DB915DD4, Vol 1