Article Sidebar

Submitted
28 December 2017
Accepted
28 December 2017
Published
28 December 2017
Download
PDF
Statistic
Read Counter : 1250 Download : 742

Main Article Content

Abstract

HPV infection is known to cause cervical cancer. This study aimed to identify the variant of HPV genotypes of cervical precancerous lesions from low grade squamous intraepithelial lesion  (LSIL) and high grade squamous intraepithelial lesion (HSIL). This was an explorative study using formalin fix paraffin embedded (FFPE) from cervical precancerous lesions at Dr. Soetomo Hospital, Surabaya. DNA was extracted from FFPE and hybridized for HPV genotyping using Ampliquality HPV Type Express kit (AB ANALITICA) by reverse line blot techniques. The results showed that there were variants of HPV genotype in LSIL. The variants were HPV16 (8/15), HPV18 (3/15), HPV52 (1/15), HPV6+31 (1/15), HPV6+18 (1/15), and HPV72+68 (1/15), and in HSIL which were HPV16 (4/10), HPV18 (2/10), HPV59 (1/10), HPV6+45 (1/10), HPV61+26 (1/10), and HPV16+31 (1/10). The characteristics of infection in LSIL were single infection of high-risk (hr) HPV and multiple infection of  low-risk (lr)+hr HPV, and in HSIL were single infection of HPVhr, multiple infection of HPVhr+hr and HPVlr+hr. In conclusion, HPV prevalence in cervical precancerous lesions is single infection by HPV16 (48%), HPV18 (20%), HPV52 (4%), HPV59 (4%), and multiple infection by HPV6+31, HPV6+18, HPV6+45, HPV16+31, HPV61+26, HPV72+68 is 4%.

Keywords

HPV genotypes LSIL HSIL

Article Details

How to Cite
Prasetyo, R. E., Mastutik, G., & Mustokoweni, S. (2017). PREVALENCE OF HUMAN PAPILLOMAVIRUS GENOTYPES IN LOW AND HIGH GRADE SQUAMOUS INTRAEPITHELIAL LESIONS AT CERVICAL TISSUE. Folia Medica Indonesiana, 53(4), 258–263. https://doi.org/10.20473/fmi.v53i4.7157
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Cosette MW, Wiliam CH, Mark S, Philip E. C., 2016. human papillomavirus genotypes and the cumulative 2-years risk of cervical precancer. Division of Cancer Epidemiology and Genetic and National Cancer Institute, 1-8
  2. Ministry of Health (2014). Hospital Information System
  3. Monia A, Emna E, Hajar L, et al (2016). Prevalence genotype distribution and risk factors for cervical human papillomavirus infection in the Grand Tunis Region, Tunisia. PloS One Journal 11, 1-12
  4. Markowitz LE, Liu G, Hariri S, Steinau M, Dunne EF, Unger ER (2016). Prevalence of HPV after introduction of the vaccination program in the United States. Pediatrics 137, e20151968. doi: 10.1542/ peds.2015-1968
  5. Morshed K, Gruszka DP, Szymanski M, Dacewicz MP (2014). Human papillomavirus (HPV) – structure, epidemiologi, dan pathogenesis. Elsevier Urban & Partner Sp. z o.o., 213-220
  6. Ogembo RK, Philimon NG, Alaina JS, et al (2015). Prevalence of human papillomavirus genotypes among African women with normal cervical cytology and neoplasia: a systematic review and meta-analysis. Plos One 10, 1-16
  7. Pablo CZ, Sebastian OR, Joaquin MB, et al (2009). Genotype distribution of human papillomavirus (HPV) and co-infection in cervical cytologic specimens from two outpatient gynecological clinics in a region of South East Spain. BMC Infectious Diseases, 1-6
  8. Rahmawati A, Damayanti, P CRS, Lumintang H (2014). Correlation of expression P16 on genital wart with various degress of cervical dysplasia. BIKKK - Berkala Ilmu Kesehatan Kulit dan Kelamin - Periodical of Dermatology and Venereology 26, 183-189
  9. Sahiratmadja E (2014). Multiple human papilloma virus infections predominant in squamous cell cervical carcinoma in Bandung 33, 33-43
  10. Schellekens MC (2004). Prevalence of single and multiple HPV types in cervical carcinomas in Jakarta, Indonesia. Gynecologic Oncology 93, 49–53
  11. Sherris J, Wittet S, Kleine A, et al (2009). Evidence-based, alternative cervical cancer screening approach-es in low-resource settings. International Journal of Gynecology & Obstetrics 35, 147-151
  12. Smith JS, Lisa L, Broke H, et al (2007). Human papillo-mavirus type distribution in invasive cervical cancer and high-grade cervical lesions: a meta-analysis update. International Journal of Cancer 121, 621–632
  13. Stanley M, Lowy DR, Frazer I (2006). Chapter 12: prophylactic HPV vaccines: underlying mechanisms. Vaccine 24, 106-13
  14. Tobing MD, Sahiratmadja E, Dinda M, Hernowo BS, Susanto H (2014). Human papillomavirus genotypes profile in cervical cancer patients at Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. Asian Pac J Cancer Prev 15, 5781-5
  15. Vet JNI, de Boer MA, van den Akker BEWM, et al (2008). Prevalence of human papillomavirus in Indo-nesia: a population-based study in three regions. Br J Cancer 99, 214-218
  16. WHO, 2014. Comprehensive Cervical Cancer Control. Geneva, 58–64 and 366–378
  17. Zulqarnain B, Lei Y, Tao Y, et al (2015). Status of human papillomavirus infection in the ethnic popula-tion in Yunnan Provience China. Biomed Research International, 1-8

Most read articles by the same author(s)