Main Article Content


Escherichia coli (E. coli) is the leading cause of male genital tract infection with no symptoms of infertility. Protein E. coli pili hemagglutinin isolated from infertile male sperm with 32.2 kDa MW acts as adhesion in spermatozoa. This study aimed to prove whether E. coli pili adhesin 32.2 kDa MW is toxic to male reproductive system. Samples consisted of spermatozoa of 30 guinea pigs divided into three groups: control, immunized with E. coli pili adhesin 32.2 kDa MW protein, and transurethral infected E. coli. Observations of sperm motility, vitality and morphology were performed under a microscope. MDA levels and sperm DNA damage were measured by a spectrophotometer and comet assay method and observed using a fluorescent microscope. There was no difference between control and immunization group of E. coli pili adhesin in motility (p=0.499), vitality (p=0.817) and morphology (p=0.176); between control and transuretral infection groups in motility (p=0.000), vitality (p=0.000) and morphology (p=0.000); and between control and both treatment groups in motility (p=0.001), vitality (p=0,000) and morphology (p=0.000). Histologic analysis showed E. coli pili adhesin of 32.2 kDa MW immunization group did not suffer from testicular tissue damage, while the positive group showed a deterioration of seminiferous tubular cells. MDA levels differed between immunization group E. coli pili, transurethral infection group, and control (p=0.024) and between transurethral and control (p=0.007) groups. However, between control and immunized group with E. coli pili protein showed no difference (p=0.251). DNA damage differed (p=0.000) between immunized group with E. coli pili, transurethral infection and control group; between control and transurethral infected group (p=0.000); and between transurethral infection group and E. coli pili protein immunization group (p=0.000). However, between control and E. coli pili immunization group showed no difference (p=0.600). In conclusion, E. coli pili adhesin 32.2 kDa MW protein is not toxic for sperm quality and the quality of sperm molecules.


E. coli pili adhesin of 32.2 kDa MW guinea pigs sperm motility morphology vitality DNA damage MDA level

Article Details

How to Cite
Sukarjati, S., Amilah, S., & Sudjarwo, S. (2018). Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System. Folia Medica Indonesiana, 54(2), 146–154.


  1. Agarwal A, Saleh RA, Bedaiwy MA (2003). Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 79, 829-843
  2. Aitken RJ (1993). Analysis of lipid peroxidation mechanism in human spermatozoa. Molecular Repro-duction and Development 35, 302-315
  3. Auroux MR, Jacquest L, Mathieu D, Aurer J (1991). Is the sperm bacterial ratio a determinating factor in impairment of sperm motility: An in vitro study in man with Escherichia coli. Int.of Andrology 14, 264-270
  4. Avendan C, Oehninger S (2011). DNA fragmentation in review morphologically normal spermatozoa: How much should we be concerned in the ICSI era? Journal of Andrology 32
  5. Baumber J, Ball BA, Gravance CG, Medina V, Davies-Morel MCG (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidationm. J. androl 21, 895-902
  6. Diemer T, Weidner W, Michelmann HW, Schiver HG, Rovan E, Mayer F (1996). Influence of Escherichia coli on motility parameters of human spermatozoa in vitro. Int J of Andrology 19, 271-277
  7. Hafez ESE, Prasad MRN (1976). Functional aspect of the epididymis, in: Human semen and fertility regula-tion in men. 1st ed. St Louis, Hafez ESE (Ed). St. Louis, The CV Mosby Company, 31-42
  8. Koksal IT, Usta M, Orhan I, Abbasoglu S, Kadioglu (2003). Potential role of reactive oxygen species on testicular pathology associated with infertility. Asian J Andrology 5, 95-99
  9. Liu JH, Li HY, Cao SG, Duan YF, Li Y (2002). Influence of several urophatogenic microorganism on human sperm motilityin vitro. Asian J. Androl 4, 179-182
  10. Marchesi D, Feng HL (2007). Sperm DNA integrity from sperm to egg. Journal of Andrology 28
  11. Monga M, Robert JA (1994). Spermagglutination by bacteria: Receptor-specific interaction. J of Andrology 15, 151-156
  12. Moon HK, Moon HR, Hong SH (2001). Determination of urinary 8 hydroxy 2 deoxyguanosin as aDNA damage marker
  13. Muratori M, Piomboni P, Baldi E, Filimberti E, Pecchioli P, Moretti E, Gambera L, Baccetti B, Biagiotti R, Forti G, Maggi M (2000). Functional and ultrastructural features of DNA-fragmented human sperm. J. of Andrology 21, 903-912
  14. Ollero M, Guzman EG, Lopez MC, Sharma RK, Argarwal A, Larson K, Evenson D, Thomas Jr AJ, Alvarez JG (2001). Characterization of subset of human spermatozoa at different stages of maturation: Implications in the diagnosis and treatment of male infertility. Human Reproduktion 16, 1912-1921
  15. Sanocka D, Kurpisz M (2004). Reaction oxygen species and sperm cells. Reprod Biol Endocrinol 2, 12
  16. Schaeffer AJ (1998). Aetiophatology and pathogenesis of urogenital infection. Andrologia 30, 3-6
  17. Sikka SC (1996). Oxidative stress and role of anti-oxidants in normal and abnormal sperm function. Frontiers in Bioscience 1, e78-e86
  18. Sukarjati, Soebadi DM, Hinting A, Sujarwo (2011). Immunogenity of 32,2 kDa hemaglutinin BM protein of Escherichia coli isolated from infertil males semen. Folia Medica Indonesiana 47
  19. Sukarjati, Sujarwo, Hinting A, Soebadi DM (2010). Pengaruh Escherichia coli dan granulosit terhadap kadar reactive oxygen species secara in vitro. Berkala Penelitian Hayati 15
  20. Sukarjati (2008). Peran protein hemaglutinin pili E. coli dan lekosit pada mekanisme penurunan kualitas spermatozoa manusia. A dissertation
  21. Sukarjati, Lunardhi H (200l). Pengaruh spesies bakteri dan ratio sperma/bakteri terhadap vitalitas sperma manusia secara in vitro, Jurnal Penelitian Berkala Hayati 7
  22. Sukarjati, Lunardhi H, Hinting A (2002). Pengaruh spesies bakteri dan ratio sperma/bakteri terhadap motilitas sperma manusia secara in vitro. Jurnal Penelitian Berkala Hayati 8
  23. Sukarjati, Lunardhi H, Sujarwo (2006). Pengaruh inkubasi spermatozoa dengan spesies bakteri yang berbeda terhadap integritas membrane plasma sperma-tozoa. Seminar Nasional Biologi, ITS, Surabaya, September 9, 2006
  24. Wolff H, Panhans A, Stolz W, Mauree M (1993). Adherence of Escherichia coli to sperm: A mannose mediated phenomenon leading to agglutination of sperm and Escherichia coli. Fertility Sterility 60, 154-158
  25. Zaneveld LJD (1985). The biology of spermatozoa, presented in: Konggres National III PANDI, Jakarta, 15-39
  26. Zini A, Sigman M (2009). Are tests of sperm DNA damage clinically useful? Pros and cons. Journal of Andrology 30