RELATIONSHIP OF NON STRUCTURAL ANTIGEN 1 (NS1) EXAMINATION RESULTS TO CLINICAL SIGNS ,SYMPTOMS AND ROUTINE BLOOD EXAMINATION IN PATIENTS SUSPECTED DENGUE INFECTIONS AT INPATIENTS CLINIC OF VITA MEDIKA KEPUNG KEDIRI DISTRICTS

Acivrida Mega Charisma

= http://dx.doi.org/10.20473/ijtid.v8i1.10382
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Abstract


Early diagnosis of dengue infection is important because late diagnostic can be fatal to the patient, remembered the journey of the disease is very rapid. Currently there has been developed an examination of a non structural 1 dengue antigen (NS1) that can detect dengue viral infections earlier, even on the first day of fever. However, not all health care centers have adequate laboratory facilities for NS1 checks.Clinical symptoms and signs as well as a routine blood test are indicators that become the basis of diagnosis in health care facilities with limited facilities. This study aims to determine the relationship of NS1 examination result to clinical symptoms and signs as well as the result of routine blood tests in patients suspected dengue infection. This research uses observational analytic method with cross sectional approach. The research was conducted in clinic laboratory and inpatient clinic room of Vita Medika Kepung Kediri from November 2017 to February 2018.The number of research samples of 30 people was determined by the consecutive sampling technique. NS1 examination was done by using rapid immunochromatography test method with mono kits. Routine blood examination was done by using Micros 60. Chi square test on relationship between clinical sign and symptoms  examination of dengue with the results of NS1 examination obtained p= 0,310 (p > 0,005), while the results of chi square test on the relationship of routine blood examination results haemoglobine levels, amount of leucocyte, platelet count and hematocite of NS1 examination result obtained p value in a row p = 0,235 (p > 0,05) , p = 0,013(p < 0,05) , p = 0,028(p < 0,05)  dan p = 0,132 (p > 0,05). There was a significant correlation between leucocyte count and platelet count to NS1 antigen exanimation result , but there was no correlation between clinical signs and symptoms of dengue patients, haemoglobine level and haematocryt value on NS1 antigen examination result.


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References


Candra A. 2010. Demam berdarah dengue: epidemiologi , patogenesis , dan faktor risiko penularan dengue hemorrhagic fever. Aspirator. 2(2):110-9

Suhendro, Nainggolan L, Chen K, Pohan HT. 2009. Demam berdarah dengue. Dalam : Sudoyo AW, Setiyohadi B, Alwi I, Simadibrata M, Setiati S, editor. Buku ajar ilmu penyakit dalam jilid III edisi V. Jakarta: Pusat Penerbitan Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Indonesia. Hal 2773-9

WHO. 2011. Comprehensive Guidelines for Prevention and Control of Dengue and Dengue Haemorrhagic Fever. India: WHO press

Suwandono A, Parwati I, Irani P, Rudiman F. 2011. Perbandingan nilai diagnostik trombosit , leukosit , antigen NS1 dan antibodi IgM anti dengue. J Indon Med Assoc. 61(8):326-32

Da Costa VG, Marques SAC, Moreli ML. 2014. A meta-analysis of the diagnostic accuracy of two commercial NS1 antigen ELISA tests for early dengue virus detection. PLoS ONE. 9(4):1-12

Libraty DH, Paul RT, Darren P,Timothy PE, Siripen K, Sharone G, et al. 2002. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. JID. 186:1165-8

Sun DS, King CC, Huang HS, Shih YL, Lee CC, Tsai WJ, et al. 2007. Antiplatelet autoantibodies elicited by dengue virus non-structural protein 1 cause thrombocytopenia and mortality in mice. J. Thromb. Haemost. 5:2291

Young P.R, Hilditch P.A, etal. J. Clinical Microbiology (2000) Vol. 38, No. 3. 1053-1057

World Health Organization (WHO). De-ngue haemorrhagic fever: Diagnosis, treatment, prevention, and control. 2nd edition. Geneva, 1997; p. 1-84.

Avirutnan P, Punyadee N, Noisakran S, Komoltri C, Thiemmeca S, Auethavornanan K, et al. 2006. Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement. J Infect Dis. 193:1078-88

Kassim FM, Izati MN, Rogayah T, Apandi YM, Saat Z. 2011. Use of dengue NS1 antigen for early diagnosis of dengue virus infection. Southeast Asian Journal of Tropical Medicine and Public Health. 42(3):562-9

Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M. 2002. Enzyme-linked immunosorbent assay specific to dengue virus type 1 non-structural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol. 40:376-81

IDAI. 2012. Infeksi virus dengue. Dalam: Editor S.S. Poorwo Soedarmo, H. Garna, S.R. S. Hadinegoro & H.I. Satari. Buku ajar infeksi & pediatri tropis. Edisi 2. Jakarta: Badan Penerbit IDAI. hal155-180

Dussart P, Labeau B , Lagathu G, Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol 2006:13:1185-9

Datta S, Wattal C . Dengue NS1 antigen detection : A useful tool in early diagnosis of dengue virus infection. Indian J Med Microbiol 2010; 28:107-10

Kumarasamy V, Abdul Wahab AH, Chua SK, et al, 2007, Evaluation of commercial Dengue NS1 Antigen Capture ELISA for laboratory diagnosis of acute dengue virus infection, J.Virol . method,140, pp 75 - 79

Ahmed NH & Shobha B. 2014. Comparison of NS1 antigen detection ELISA, real time RT-PCR and virus isolation for rapid diagnosis of dengue infection in acute phase. J Vector Borne.Dis. 51:194-9

Irawan Anasta Putra, Ahmad Syauqy, Armadi Darmawan, Ave Olivia Rahman. Korelasi Pemeriksaan NS1 Ag Dan Pemeriksaan Darah Tepi Pada Anak Dengan Demam,2016. JMJ . Vol 4. 2016:106-118

Rena N, Utama S, Parwati T. 2009. Kelainan hematologi pada demam berdarah dengue. J Peny Dalam. 10:3Santosh ST, Chincholkar VV, Kulkarni DM, Nilekar SL, Ovhal RS, Halgarkar CS. 2013. A study of NS1 antigen and platelet count for early diagnosis of dengue infection. Int J Curr Microbiol App Sci. 2(12):40-4

Muhamad Jyuldi Prayoga. Hubungan Hasil Pemeriksaan Antigen Non Struktural 1 (NS1) Terhadap Gejala, Tanda Klinis dan Jumlah Trombosit Pada pasien Suspect Infeksi Dengue , 2017. Skripsi fakultas Kedokteran Universitas Lampung 2017.

Kulkarni RD, et al. 2011. Association of platelet count and serological markers of dengue infection-importance of NS1antigen. Indian J Med Microbiol. Vol 29:359-62

Megariani, Rinang Mariko, Amrin Alkavar, andani Eka Putra . Uji Diagnostik Pemeriksaan Antigen non Struktural 1 Untuk Deteksi Dini Infeksi Virus Dengue Pada anak , 2014. Sari Pediatri.Vol 16.2014

Mayer F.Wowor. Deteksi Dini Demam Berdarah Dengue Dengan Pemeriksaan Antigen NS1, 2011. Jurnal Biomedik. 2011. Vol 3 :1-9

Pusparini. 2004. Kadar hematokrit dan trombosit sebagai indikator diagnosis infeksi dengue primer dan sekunder. Jurnal Kedokteran Trisakti. 23(2):51-6


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