COMBINED TARGET SITE VGSC MUTATIONS PLAY A PRIMARY ROLE IN PYRETHROID RESISTANT PHENOTYPES OF Aedes aegypti AS DENGUE VECTOR FROM PALU CITY, CENTRAL SULAWESI

Sitti Rahmah Umniyati

= http://dx.doi.org/10.20473/ijtid.v7i5.10384
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Abstract


It has been reported that Aedes aegypti mosquitoes in Palu City had been resistant to cypermethrin insecticide but the resistance mechanism is not well known. This study aimed to determine the resistance status of Ae. aegypti to cypermethrin and whether the mutation of voltage-gated sodium channel (VGSC) was associated with pyretroid resistance in high and low dengue endemic areas in Palu City. Aedes aegypti collected from each village was reared to adult and assayed for susceptibility test to cypermethrin using the CDC bottle bioassay method. PCR primers of AaSCF1 and AaSCR4 were used for screening of IIS6 VGSC gene mutation. PCR primers of AaSCF7 and AaSCR7 were used for screening of IIIS6 VGSC gene mutation. For an identification of mutation sites were sequenced and aligned to Gene bank (access No. AB914689 and AB914690) for IIS6 VGSC and Gene bank (access No. AB914687 and AB914688) for IIIS6 VGSC gene mutation. The susceptibility status of Ae. aegypti to cypermethrin was resistant in high dengue endemic areas and moderately resistant in low dengue endemic areas. It was found double point mutation at S989P and V1016G in Ae. aegypti from high and low dengue endemic areas in Palu City and there was a single point mutation only in high dengue endemic area at target site V1016G. Aedes aegypti from both high and low dengue endemic areas were resistant to cyperpethrinn and the two alleles had a major role in the occurrence of cypermethrin resistance in Palu City.


Keywords


Aedes aegypti, resistance, pyrethroid, VGSC gene mutation

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References


WHO. Dengue Guidelines for Diagnosis, Treatment, Prevention and Control. Geneva, Switzerland: WHO; 2009.

World Health Organization. Comprehensive guidelines for prevention and control of dengue and dengue haemorrhagic fever. South-East Asia: WHO, 2011.

World Health Organization. National guidelines for clinical management of dengue fever. India: WHO, 2015.

Dinas Kesehatan Kota Palu. 2017. Pengendalian Penyakit Demam Berdarah Dengue. Peraturan Daerah (PERDA) Kota Palu No. 2 Tahun 2016. Available from: https://peraturan.bpk.go.id/Home/ Details/20800.

Kementrian Kesehatan Republik Indonesia. Pedoman penggunaan insektisida (pestisida) dalam pengendalian vektor. Jakarta: Direktorat Jendral Pengendalian dan Penyehatan Lingkungan, 2012. Available from:http://perpus.b2p2vrp.litbang.depkes.go.id/opac/index. php?p=show_detail&id=1107&keywords=

Yuliani TS, Triwidodo H, Mudikdjo K, Panjaitan, NK, Sjafrida Manuwoto S. Pestisida rumah tangga untuk pengendalian hama permukiman pada rumah tangga. JPSL, 2011;2 (1): 73-83.

Jastal. Pemetaan status resistensi insektisida di Indonesia tahun 2015. Laporan penelitian. Balai Litbangkes Donggala, 2015. Available from: https://docplayer.info/44333679-Laporan-tahunan-tahun-balai-penelitian-dan-pengembangan-pengendalian-penyakit-bersumber-binatang-donggala.html

Smith LB, Kasai S, Scott JG. Pyrethroid resistance in Aedes aegypti and Aedes albopictus: Important mosquito vectors of human diseases. Pestic. Biochem. Physiol. 2016. 133: 1-12.

Sastroasmoro S dan Ismail S. Dasar-dasar metodologi penelitian

klinis. 5th Ed. Jakarta: CV. Sagung Seto. 2014. 104-110.

World Health Organization. Monitoring and managing insecticide resistance in Aedes mosquito populations. Genewa: WHO, 2016. Available from:https://www.who.int/csr/resources/publications/zika/ insecticide-resistance/en/

Center for Desease Control and Prevention Gudline for evaluating insecticide resistance in vector using the CDC bottle bioassay. USA: CDC, 2010. 1-83.

Anonymus. Genomic DNA Mini Kit (Blood/Cultured Cell). Geneaid www.geneaid.com Available from: Geneaid www.geneaid.com. http://www.geneaid.com/products/genomic-dna-purification/dna-extraction-kit-blood-cultured-cell-miniprep.

Kawada H, Oo SZM, Thaung S, Kawashima E, Maung YNM, Thu HM, et al. (2014) Co-occurrence of Point Mutations in the Voltage-Gated Sodium Channel of Pyrethroid-Resistant Aedes aegypti Populations in Myanmar. PLoS Negl Trop Dis 8(7): e3032. https:// doi.org/10.1371/journal.pntd.0003032.

Ponlawat A, Scott JG, Harington LC. Insecticide susceptibility of Aedes aegypti an Aedes albopictus across Thailand. J Med Entomol. 2015; 42: 821-825.

Chareonviriyaphap T, Bangs MJ, Suwonkerd W, Kongmee M, Corbel V, Ngoen-Klan R. Review of insecticide resistance and behavioral avoidance of vectors of human diseases in Thailand. Parasit Vectors. 2013. 6: 280. doi: 10.1186/1756-3305-6-280

Seixas G, Grikoraki L, Weetman D, Vicente JL, Silva AC, Pinto J, Vontas J, Sousa JA. Insecticide resistance is mediated by multiple mechanisms in recently introduced Aedes aegypti from Madeira Island (Portugal). PLoS Negl Trop Dis. 2017. https://doi.org/10.1371/ journal.pntd.0005799

Ahmad I. Adapatsi serangga dan dampaknya terhadap kehidupan Manusia. 2011.Pidato ilmiah Guru Besar Institut Teknologi Bandung (ITB). Available from: http://rektorkita.itb.ac.id/files/ balon/IA_CV.pdf

Mantolu Y, Kustiati, Ambarningrum, TB, Yusmalinar S, Ahmad I. Status dan perkembangan resistensi Ae. aegypti (Linnaeus) (Diptera: Culicidae) Strain Bandung, Bogor, Makassar, Palu dan VCRU terhadap tnsektisida permetrin dengan seleksi lima generasi. J Entomol Indonesi. 2018. 13(1):1-8.

Amelia-Yap ZH, Ahee DC, Azirun MS, Van LL, Pyrethroid resistance in the dengue vector Aedes aegypti in Southeast Asia: present situation and prospects for management. Parasite Vectors.2018. 11.332:1-17. doi: 10.1186/s13071-018-2899-0

Alvarez LC, Ponce G, Oviedo M, Lopez B, Flores AE. Resistance to malation and deltamethrin in Aedes aegypti (Diptera : Culicidae) From Western Venezuela. Entomol Soc Am. 2013. 50(5):1031–9.

Widiarti, Heriyanto B, dan Boewono, DT. Peta resistensi vektor demam berdarah dengue Aedes aegypti terhadap insektisida kelompok organophosfat, karbamat dan piretroid di Provinsi Jawa Tengah dan Yogyakarta. Buletin Penelitian Kesehatan. 2011. (4): 39.

Sunaryo, Ikawati B, Rahmawati., Widiastuti D. Status resistensi vektor demam berdarah dengue (Aedes Aegypti) terhadap malation 0,8% dan permethrin 0,25% di Provinsi Jawa Tengah. Ekologi Kesehatan. 2014. Vol. 13 No. 2.

Ikawati B, Sunaryo,Widiastuti D. Peta status kerentanan Aedes aegypti (Linn.) terhadap insektisida cypermethrin dan malation di Jawa Tengah. Aspirator. 2015. 7(1): 23-8.

Stenhouse SA, Plernsub S, Yanola J, Lumjuan N, Dantrakool A, Choochote W, Somboon P. Detection of the V1016G mutation in the voltage-gated sodium channel gene of Aedes aegypti (Diptera: Culicidae) by Allele-Specific PCR Assay, and its distribution and effect on deltamethrin resistance in Thailand. Parasites & Vectors. 2013.6: 253.

Rajatileka S, William CB, Saavedra KR, Yuwadee T, Chamnarn A, McCalla PJ, and Hilary R. Development and application of a simple colorimetric assay reveals widespread distribution of sodium shannel mutations in thai populations of Aedes aegypti. Acta Tropica. 2008. 108: 54–7.

Srisawat R, Komalamisra N, Eshita Y, Zheng M, Ono K, Itoh TQ, Matsumoto A, Petmitr S, and Rongsriyam Y. Point mutations in domain II of the voltage-gated sodium channel gene in deltamethrin-resistant Aedes aegypti (diptera: Culicidae). Appl. Entomol. Zool. 2010; 45. 275–282.

Al Nazawi AM, Aqili J, Alzahrani M, McCall PJ, and Weetman D. Combined target site (kdr) mutations play a primary role in highly pyrethroid resistant phenotypes of Aedes aegypti from Saudi Arabia. Parasites & Vectors. 2017;10:161.

Martins AJ, Lima JB, Peixoto AA, and Valle D. Frequency of Val1016Ile mutation in the voltage-gated sodium channel gene of Aedes aegypti Brazilian populations. Trop Med Int Health. 2009.14(11): 1351-5, ISSN 1365-3156.

Lima EP, Paiva MHS, De Araújo AP, Da Silva ÉVG, Da Silva UM., De Oliveira A.E.G. Santana, C.N.Barbosa, C.C.P. Neto, M.O.F Goulart, C.S. Wilding, Ayres LN, and Santos MAVM. Insecticide resistance in Aedes aegypti populations from Ceará, Brazil. Parasites & Vectors. 2011. 4(5):1-12.

Ghiffari A, Fatimi H, dan Anwar C. Deteksi resistensi insektisida sintetik piretroid pada Aedes aegypti (l.) strain Palembang menggunakan teknik polymerase chain reaction. Aspirator. 2013. 5

(2): 37-44.

Ishak IH, Jaal Z, Ranson H, and Wondji CS. contrasting patterns of insecticide resistance and knockdown resistance (kdr) in the dengue vectors Aedes aegypti and Aedes albopictus from Malaysia. Parasites & Vectors. 2015. 8:181.

Plernsub S, Saingamsook J, Yanola J, Lumjuan N, Tippawangkoso P,Sukontason K, Walton C, and Somboon P. Additive effect of knockdown resistance mutations, S989P, V1016G and F1534C,in a heterozygous genotype conferring pyrethroid resistance in Aedes aegypti in Thailand. Parasites & Vectors, 2016; 9:417.

Widyastuti D, Sunaryo, Pramestuti N, Sari TF, Wijayanti N. Deteksi mutasi V1016G pada gen voltage-gated sodium channel pada populasi Aedes aegypti (Diptera: Culicidae) di Kabupaten Klaten, Jawa Tengah dengan metode Allele-Specific PCR. Vektora. 2015. 7 (2): 65-70.


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