Humaryanto Humaryanto, Hanina Hanina, Lipinwati Lipinwati, Charles Apul Simanjuntak

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Staphylococcal cassette chromosome mec (SCCmec) is one of the mobile genetic elements of Methicillin-Resistant Staphylococcus aureus (MRSA) that carries many resistance genes and allows SCCmec to move from one bacterium to another. Twelve types of SCCmec have been identified throughout the world. Identification of SCCmec type is needed to determine the pattern of MRSA resistance in a particular region. This study aimed to identify the type of SCCmec MRSA from clinical samples. Specifically, this study was conducted at the Biomolecular Laboratory of the Faculty of Medicine and Health Sciences of Jambi University in June 2018-February 2019. Culture was carried out on 100 clinical specimens of festering wound swabs from inpatients at hopitals in Jambi City. A total of 32 samples of Staphytect plus test positive were tested using Cefoxitin disc diffusion method and MecA Polymerase Chain Reaction (PCR). There were 14 samples identified as MRSA isolates, namely twelve samples (85.72%) of SCCmec type III, one sample (7.14%) of SCCmec type II, and one sample (7.14%) of SCCmec type IVb. The results were different from previous studies where all MRSA isolates (100%) in Indonesia were SCCmec type III, although most SCCmec types were still dominated by SCCmec type III. This study concludes that there has been a shift in the content of SCCmec in MRSA isolate originating from hospitals in Jambi city.


MRSA; mecA; SCC mec, genetics, resistence

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Alrabiah K, Alola S Al, Banyan E Al, Shaalan M Al, Johani S Al. Characteristics and risk factors of hospital acquired e Methicillin-resistant Staphylococcus aureus ( HA-MRSA ) infection of pediatric patients in a tertiary care hospital in Riyadh , Saudi Arabia. Int J Pediatr Adolesc Med. Elsevier Ltd; 2016;3(2):71–7.

Chambers HF. Methicillin Resistance in Staphylococci : Molecular and Biochemical Basis and Clinical Implications. Clin Microbiol Rev. 1997;10(4):781–91.

Liana P. Gambaran Kuman Methicillin-Resistant Staphylococcus Aureus ( MRSA ) di Laboratorium Mikrobiologi Departemen Patologi Klinik Rumah Sakit Dr. Cipto Mangunkusumo (RSCM) Periode Januari-Desember 2010. MKS. 2014;46(3):171–5.

Mahmudah R, Soleha TU, Ekowati C. Identifikasi Methicillin Resistant Staphylococcus aureus (MRSA) pada Tenaga Medis dan Paramedis di Ruang Intesive Care Unit (ICU) dan Ruang Perawatan Bedah Rumah Sakit Umum Daerah Abdoel Moeloek. Med J Lampung Univ. 2013;2(4):70–8.

Monecke S, Schwarz S, Hotzel H, Ehricht R. Rapid Microarray-Based Identification of Different mecA Alleles in. 2012;56(11):5547–54.

Chongtrakool P, Ito T, Ma XX, Kondo Y, Trakulsomboon S, Tiensasitorn C, et al. Staphylococcal Cassette Chromosome mec ( SCC mec ) Typing of Methicillin-Resistant Staphylococcus aureus Strains Isolated in 11 Asian Countries : a Proposal for a New Nomenclature for SCC mec Elements. Antimicrob Agents Chemother. 2006;50(3):1001–12.

Ito T, Hiramatsu K, Oliveira DC, De Lencastre H, Zhang K, Westh H, et al. Classification of staphylococcal cassette chromosome mec (SCCmec): Guidelines for reporting novel SCCmec elements. Antimicrob Agents Chemother. 2009;53(12):4961–7.

Lyytikainen O, Jagerroos H, Vuopio-Varkila J, Virolainen A, Salmenlinna S, Ibrahem S, et al. Carriage of Methicillin-Resistant Staphylococci and Their SCCmec Types in a Long-Term-Care Facility. J Clin Microbiol. 2008;47(1):32–7.

Nitschke H, Pfohl K, Monecke S, Jatzwauk L, Mu E, Slickers P, et al. Diversity of SCC mec Elements in Staphylococcus aureus as Observed in South- Eastern Germany. PLoS One. 2016;11(9):1–24.

Yuwono, Sunarjati S, Masria S, Supardi I. Staphylococcus aureus dengan Polymerase Chain Reaction Identification of Staphylococcal Cassette Chromosome Mec Methicillin Resistant Staphylococcus aureus Using Polymerase Chain Reaction. Maj Kedokt Bandung. 2009;43(2):60–5.

Sudigdoadi S. Analisis Tipe Staphylococcal Cassette Chromosome mec (SCCmec) Isolat Methicillin Resistant Staphylococcus aureus (MRSA). Maj Kedokt Bandung. 2014;42(4):149–54.

Cockerill FR, Wikler MA, Bush K, Craig WA, Dudley MN, Eliopoulos GM, et al. Performance Standards for Antimicrobial Susceptibility Testing ; Twenty-First Informational Supplement. Vol. 31, CLSI document. 2011. M100-S21 p.

McClure-Warnier J-A, Conly JM, Zhang K. Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus. J Vis Exp. 2013;(79).

Peters B, Liu J, Chen D, Peters BM, Li L, Li B, et al. Staphylococcal chromosomal cassettes mec ( SCCmec ): A mobile genetic element in methicillin-resistant Staphylococcus aureus Microbial Pathogenesis Staphylococcal chromosomal cassettes mec ( SCC mec ): A mobile genetic element in methicillin-resistant Staphylococcus aureus. Microb Pathog. Elsevier Ltd; 2016;101(July 2018):56–67.

Ghanbari F, Saberianpour S, Ghanbari N. Staphylococcal Cassette Chromosome mec ( SCC mec ) Typing of Methicillin-Resistant Staphylococcus aureus Strains Isolated from Community- and Hospital-Acquired Infections. Avicenna J Clin Microb Infec. 2017;4(2).

Sabir M, Dwiyanti R, Hatta M, Buntaran L, Sultan AR. Sccmec type II gene is common among clinical isolates of methicillin-resistant Staphylococcus aureus in Jakarta, Indonesia. BMC Res Notes. 2013;6(1):110.

Donnio P, Bifani P, Dehem M, Wilhelm N, Lafforgue N, Cormier M, et al. Molecular and Epidemiological Evidence for Spread of Multiresistant Methicillin-Susceptible Staphylococcus aureus Strains in Hospitals. Antimicrob Agents Chemother. 2007;51(12):4342–50.

Tenover FC, Mcdougal LK, Goering R V, Killgore G, Projan SJ, Patel JB, et al. Characterization of a Strain of Community-Associated Methicillin-Resistant Staphylococcus aureus Widely Disseminated in the United States †. J Clin Microbiol. 2006;44(1):108–18.

Ghaznavi-rad E, Ekrami A. Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolates , Isolated from a Burn Hospital in Southwest Iran in 2006 and 2014. Hindawi Int J Microbiol. 2018;

Hannan A, Javed F, Saleem S, Tahira K, Jahan S. Frequency of Staphylococcal Cassette Chromosome mec Type IV and Type V in Clinical Isolates of Methicillin Resistant Staphylococcus aureus. Open J Med Microbiol. 2015;5(June):69–75.


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