Germ Tube Induction Test Comparing Total of Six Liquid and Three Solid Media in Candida albicans
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Invasive candidiasis (IC) has a high mortality rate of 70%, thus diagnosis should be established without delay. Given its fast result, serological test such as β-d-glucan (BDG) test is one alternative diagnosis modalities. However, it lacks specificity. Candida albicans germ tube antibody (CAGTA) test is an alternative serological test which has a high sensitivity of 76.2% and specificity of 80.3%. Manufacturing CAGTA serological test requires provision of specific germ tube antigen. In this study, various culture media were tested to find the best media for germ tube induction. This study was an experimental in vitro study. The number and length of the germ tube were recorded in two- and three-hour incubation periods. A total of six samples containing one C. albicans ATCC 90028, four C. albicans wild type strains, and one C. krusei wild type strain were used. Nine media were tested to induce germ tube formation: human and sheep serum, fetal bovine serum, mueller hinton agar and broth, tryptic soy agar and broth, brain heart infusion agar and broth. At both incubation periods, the medium with the highest number of germ tube was human serum (p=0.001 and p=0). The longest germ tube was found in sheep serum at two-hour incubation period (p=0.005). Mueller hinton broth (MHB) showed comparable results with human and sheep serum (p>0.05). Human serum is a superior inducer of morphogenesis. However, the use of MHB is recommended in this study, since provision of fresh human and sheep serum on a regular basis is impractical.
Bongomin F, Gago S, Oladele RO, Denning DW. Global and multi-national prevalence of fungal diseases”estimate precision. Journal of Fungi. 2017;3(4):57.
Clancy CJ, Nguyen MH. Diagnosing invasive candidiasis. Journal of Clinical Microbiology. 2018;56(5):e01909-17.
Loreto ES, Tondolo JSM. Fungal Infection. London, UK: Intechopen; 2019.
Lockhart SR. Current Epidemiology of Candida Infection. Clin Microbiol Newsl. 2014;36(17):131–6.
Yapar N. Epidemiology and risk factors for invasive candidiasis. Therapeutics and Clinical Risk Management. 2014;10:95- 105.
Henriques M, Silva S. Candida albicans Virulence Factors and Its Pathogenicity. Microorganisms. 2021;9(4):704.
Hage C, Carmona E, Epelbaum O, Evans S, Gabe L, Haydour Q et al. Microbiological Laboratory Testing in the Diagnosis of Fungal Infections in Pulmonary and Critical Care Practice. An Official American Thoracic Society Clinical Practice Guideline. American Journal of Respiratory and Critical Care Medicine. 2019;200(5):535-550.
Parra-Sánchez M, Zakariya-Yousef Breval I, Castro Méndez C, García-Rey S, Loza Vazquez A, íšbeda Iglesias A, et al. Candida albicans Germ-Tube Antibody: Evaluation of a New Automatic Assay for Diagnosing Invasive Candidiasis in ICU Patients. Mycopathologia. 2017;182(7– 8):645–52.
Villa S, Hamideh M, Weinstock A, Qasim M, Hazbun T, Sellam A et al. Transcriptional control of hyphal morphogenesis in Candida albicans. FEMS Yeast Research. 2020;20(1).
Mayer FL, Wilson D, Hube B. Candida albicans pathogenicity mechanisms. Virulence. 2013;4:2 119-128.
Pini P, Colombari B, Marchi E, Castagnoli A, Venturelli C, Sarti M et al. Performance of Candida albicans germ tube antibodies (CAGTA) and its association with (1 → 3)-β-D-glucan (BDG) for diagnosis daof invasive candidiasis (IC). Diagnostic Microbiology and Infectious Disease. 2019;93(1):39-43.
Mehta A, Kumar M, Bhumbla U, Vyas A, Dalal AS. Comparison of Different Media for Germ Tube Production by Candida albicans: A Retrospective Study. Int J Curr Microbiol Appl Sci. 2018;7(6):819– 23.
Wohlmeister D, Vianna D, Helfer V, Calil L, Buffon A, Fuentefria A et al. Differentiation of Candida albicans , Candida glabrata , and Candida krusei by FT-IR and chemometrics by CHROMagarâ„¢ Candida. Journal of Microbiological Methods. 2017;141:121-125.
Silva S, Negri M, Henriques M, Oliveira R, Williams D, Azeredo J. Candida glabrata, Candida parapsilosisandCandida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance. FEMS Microbiology Reviews. 2012;36(2):288-305.
Gómez-Gaviria M, Mora-Montes HM. Current Aspects in the Biology, Pathogeny, and Treatment of Candida krusei, a Neglected Fungal Pathogen. Infect Drug Resist. 2020;13:1673-1689. Published 2020 Jun 10. doi:10.2147/IDR.S247944
Procedure for Serum Processing from Whole Blood. Brd.nci.nih.gov.https://brd.nci.nih.gov/brd/sop/download-pdf/148. Published 2020. Accessed January 28, 2021.
Audreylia E, Budiman Y, Surja SS. Mentha piperita extract, a potential antifungal agent against Candida albicans and Candida krusei. Curr Res Environ Appl Mycol. 2020;10(1):236–41.
Atalay MA, Koc AN, Parkan OM, Aydemir G, Elmali F, Sav H. Can serums be replaced by Mueller-Hinton agar in germ tube test? Niger J Clin Pract. 2017;20(1):61–3.
Barbedo JGA. Automatic Object Counting In Neubauer Chambers. Brazilian Telecommunications. 2013.
Tsui C, Kong E, Jabra-Rizk M. Pathogenesis of Candida albicans biofilm. Pathogens and Disease. 2016;74(4).
Chen H, Zhou X, Ren B, Cheng L. The regulation of hyphae growth in Candida albicans. Virulence. 2020;11(1):337-348.
Garbe E, Vylkova S. Role of Amino Acid Metabolism in the Virulence of Human Pathogenic Fungi. Current Clinical Microbiology Reports. 2019;6(3):108-119.
Burch J, Mashayekh S, Wykoff D, Grimes C. Bacterial Derived Carbohydrates Bind Cyr1 and Trigger Hyphal Growth in Candida albicans. ACS Infectious Diseases. 2018;4(1):53-58.
Hudson DA, Sciascia QL, Sanders RJ, Norris GE, Edwards PJB, Sullivan PA, et al. Identification of the dialysable serum inducer of germ-tube formation in Candida albicans. Microbiology. 2004;150(9):3041–9.
Van Ende M, Wijnants S, Van Dijck P. Sugar Sensing and Signaling in Candida albicans and Candida glabrata. Frontiers in Microbiology. 2019;10.
Seo D, Paek SH, Oh S, Seo S, Paek SH. A human serum-based enzyme-free continuous glucose monitoring technique using a needle-type bio-layer interference sensor. Sensors (Switzerland). 2016;16(10):1581.
AL-Hadithy HA, Badawi NM. Determination of Serum Proteins and Glucose Concentrations in Clinically Normal and Anemic Awassi Sheep. World s Veterinary Journal. 2015;6(1):01.
Branzoi I, Iordoc M, Branzoi F, Vasilescu-Mirea R, Sbarcea G. Influence of diamond-like carbon coating on the corrosion resistance of the NITINOL shape memory alloy. Surface and Interface Analysis. 2010;42(6-7):502-509.
Su C, Yu J, Lu Y. Hyphal development in Candida albicans from different cell states. Current Genetics. 2018;64(6):1239-1243.
Hilmioglu S, Ilkit M, Badak Z. Comparison of 12 liquid media for germ tube production of Candida albicans and C. tropicalis. Mycoses. 2007;50(4):282– 5.
Ding X, Liu Z, Su J, Yan D. Human serum inhibits adhesion and biofilm formation in Candida albicans. BMC Microbiol. 2014;14(1):80.
Mba I, Nweze E. Mechanism of Candida pathogenesis: revisiting the vital drivers. European Journal of Clinical Microbiology & Infectious Diseases. 2020;39(10):1797-1819.
Wich M, Greim S, Ferreira-Gomes M, Krüger T, Kniemeyer O, Brakhage A et al. Functionality of the human antibody response to Candida albicans. Virulence. 2021;12(1):3137-3148.
Becton Dickinson and Company. Manual of Microbiological Culture Media - DB (2nd edition). Difco & BBL Manual Manual of Microbiological Culture Media. 2009.
Yakasiri HP, Siddabathuni A. Utility of non-serum liquid media against conventional human serum in germ tube production test. IP International Journal of Medical Microbiology and Tropical Diseases. 2020;6(1):54-57.
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