EVALUATION OF Salmonella sp CONTAMINATION AND ITS ANTIBIOTICS RESISTANCE PATTERNS ISOLATED FROM BROILER MEAT SOLD AT WET MARKET IN CENTER OF SURABAYA
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Antibiotic resistance now days become a main issue to the medical researches as found many positive result of antibiotic resistance test. One of the causes of antibiotic resistance is using antibiotic as a feed additive to animal. Bacteria that are resistant to antibiotics can be a danger to humans, in this case the resistant bacteria as a result of treatment errors animals, especially chickens that uses low-dose antibiotics as growth promoters. This study aimed to determine the contamination of Salmonella sp and its antibiotics resistance patterns of Salmonella sp isolated from broiler meat sold at wet market in the Center of Surabaya: (a) Pasar Kembang, (b) Pasar Kupang, (c) Pasar Dukuh Kupang, (d) Pasar Kedungsari, (e) Pasar Kedungdoro and (f) Pasar Keputran. The method that used in this study was bacteriological isolation and identification method. The method started with pre-enrichment using Buffered Pepton Water, selective enrichment using Tetrathionate Broth and Selenite Cysteine broth, selective media using Salmonella-Shigella Agar, Biochemical test using Triple Sugar Iron Agar, Simon Citrate, Methyl Red – Voges Proskauer, and Sulfide Indol Motility, and followed with susceptibility test according to Kirby-Bauer method using Mueller-Hinton Agar. The antibiotics that used in susceptibility test were: (a) Meropenem, (b) Ampicillin Sulbactam, (c) Amikacin, (d) Ofloxacin and (e) Nalidixic Acid. The results of this study were
found 90% or 27 of 30 samples positive contaminated with Salmonella sp. The results of antibiotics resistance from 27 isolates 0% were resistant to Meropenem, 0% were resistant to Amikacin; 3.7% were resistant to Ampicillin-Sulbactam; 11.1% were resistant to Ofloxacin and 44.4% were resistant to Nalidixic Acid.
Direktorat Jenderal Peternakan, Departemen Pertanian (Ditjennak). 2008. Statistik Peternakan. Jakarta: Direktorat Jenderal Peternakan.
Lawrie RA, 2003. Ilmu Daging. Edisi Kelima. Universitas Indonesia Press, Jakarta. p. 132–157.
World Health Organization (WHO). 2014. Salmonella. http://www.who.int/topics/salmonella/en/. [16 September 2014].
Crump JA, SP. Luby, and ED. Mintz. 2004. The Global Burden of Typhoid Fever. Bull World Health Organ 82: 346–353.
Departemen Kesehatan RI (Depkes RI). 2009. Profil Kesehatan Indonesia Tahun 2008. Jakarta: Depkes RI.
Fitria Y, RH. Nugroho, HB. Sosiawan, Noviarti, dan Nurhayati. 2004. Hasil Monitoring dan Surveilanse Cemaran Mikroba dan Residu Antibiotika di Kota Padang, Pekanbaru dan Jambi. Tahun 2004. Informasi Kesehatan Hewan.
Mulyana, Yanti. 2007. Sensitivitas Salmonella sp. Penyebab Demam Tifoid terhadap Beberapa Antibiotik di Rumah Sakit Immanuel Bandung. Bandung: Fakultas Kedokteran Universitas Padjajaran.
Bell C, and A. Kyriakides. 2002. Salmonella a Practical Approach to the Organism and its Control in Foods. UK: Blackwell Science ltd.
Reynolds J. 2012. Kirby-Bauer (Antibiotic Sensitivity). Dallas, USA: Richland College.
Soeparno. 2005. Ilmu dan Teknologi Daging. Yogyakarta: Gadjah Mada University Press. p. 113–114.
Kalalo LP, Aryati, dan B. Subagjo. 2004. Pola Bakteri dan Tes Kepekaan Antibiotika Wanita Hamil dengan Bakteriuria Asimtomatis. Surabaya: Universitas Airlangga.
Suyatna F. dan H. Toni. 1995. Farmakologi dan Terapi: Edisi Keempat. Jakarta: Penerbit Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia. p. 595.
Center of Disease Control and Prevention (CDC). 2013. Antibiotics Resistance Threat in the United States. USA: CDC. p. 23.
Pratiwi ST. 2008. Mikrobiologi Farmasi. Penerbit Erlangga: Jakarta. p. 136; 149–160; 165–171.
Russell AD and I. Chopra. 1990. Understanding Antibacterial Action and Resistance. New York: Ellis Horwood series in Pharmaceutical Technology.
Rang HP and MM. Dale. 1991. Pharmacology. UK: Churchill Livingstone. p. 824–825.
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