Diagnostic Test of Blood Eosinophil Level as a Marker of Ascaris lumbricoides Infection
Downloads
A. lumbricoides infection is one from 17 neglected tropical diseases in Indonesia. But, Infection of A. lumbricoides in Indonesia often undiagnosed. This is because stool examination with Kato-Katz's method still rarely done. So it needs something alternative examination that more practical and can be routinely carried out. This study was a diagnostic test for blood eosinofil levels in diagnosing A. lumbricoides infection. This research was conducted at a hospital in Medan. This study involved 63 children who had their parents approval. Children stool were examined by Kato-Katz method as a gold standard and blood eosinofil levels was examined as an index in this study. The results showed sensitivity level of eosinofil is 25% and specificity 96,08%, positive predictive value 60%, negative predictive value 84%, positive likelihood ratio 6,38% and negative likelihood ratio 0,78%. The conclusion is elevated blood eosinofil levels cannot be used as an alternative test Kato-Katz in diagnosing Infection of A. lumbricoides.
de Silva, N., Brooker, S., Hotez, P., Montresor, A., Engels, D. and Savioli, L. Soil-transmitted helminth infections: updating the global picture. Trends in Parasitology, 2003, 19(12), 547-51.
Siregar CD. Pengaruh Infeksi Cacing Usus pada Pertumbuhan Fisik Anak Usia Sekolah Dasar. Sari Pediatri, 2006, 8 (2), 112-7.
[WHO] World Health Organization. Assessing the epidemiology of soil-transmitted helminths during a transmission assessment survey (TAS) in the global programme for the elimination of lymphatic filariasis. Geneva: Preventive Chemotherapy and Transmission Control (PCT) Department of Control of Neglected Tropical Diseases (NTD) World Health Organization, 2015.
Klion, A. and Nutman, T. The role of eosinophils in host defense against helminth parasites. Journal of Allergy and Clinical Immunology, 2004, 113(1), 30-37.
MacDonald, A.S., Araujo M.I., Pearce E.J. Immunology of Parasitic Helminth Infections. Infection and Immunity, 2002, 70(2), 427-433
Shin, M., Lee, Y. and Min, D. Eosinophil-Mediated Tissue Inflammatory Responses in Helminth Infection. The Korean Journal of Parasitology, 2009, 47(Suppl), p.S125.
Barda, B., Albonico, M., Ianniello, D., Ame, S., Keiser, J., Speich, B., Rinaldi, L., Cringoli, G., Burioni, R., Montresor, A. and Utzinger, J. How Long Can Stool Samples Be Fixed for an Accurate Diagnosis of Soil-Transmitted Helminth Infection Using Mini-FLOTAC?. PLOS Neglected Tropical Diseases, 2015, 9(4), p.e0003698.
Nutman, T.B. Evaluation and Differential Diagnosis of Marked, Persistent Eosinophilia. Immunology and Allergy Clinics of North America, 2007, 27(3), 529-549.
Lacy P, Adamko DJ, Moqbel R. The Human Eosinophil, 2014. In: Greer, ed. Wintrobe's Clinical Hematology. 13th ed. Philadelphia, PA: Lippincott Williams & Wilkins. 363-411
[UNICEF] United Nations Children's Fund , East Asia and Pacific Regional Office. Mapping human helminth infections in Southeast Asia. Bangkok. UNICEF East Asia & Pacific Regional Office, 2003.
Simarmata, N. Nutritional status of soil-transmitted helminthiasis-infected and uninfected children. Paediatrica Indonesiana, 2015, 55 (3), 136-41
Bergquist, R., Johansen, M. and Utzinger, J. Diagnostic dilemmas in helminthology: what tools to use and when?. Trends in Parasitology, 2009, 25(4), 151-156.
Tarafder, M., Carabin, H., Joseph, L., Balolong, E., Olveda, R. and McGarvey, S. Estimating the sensitivity and specificity of Kato-Katz stool examination technique for detection of hookworms, Ascaris lumbricoides and Trichuris trichiura infections in humans in the absence of a ‘gold standard'. International Journal for Parasitology, 2010, 40(4), 399-404.
[WHO] World Health Organization. Bench aids for the diagnosis of intestinal parasites. Geneva : World Health Organization, 2012.
Barda, B., Cajal, P., Villagran, E., Cimino, R., Juarez, M., Krolewiecki, A., Rinaldi, L., Cringoli, G., Burioni, R. and Albonico, M. Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina. Parasites & Vectors, 2014, 7(1), p.271.
Periago, M., Diniz, R., Pinto, S., Yakovleva, A., Correa-Oliveira, R., Diemert, D. and Bethony, J. The Right Tool for the Job: Detection of Soil-Transmitted Helminths in Areas Co-endemic for Other Helminths. PLOS Neglected Tropical Diseases, 2015, 9(8), p.e0003967.
Levecke, B., Behnke, J. M., Ajjampur, S. S., Albonico, M., Ame, S. M., Charlier, J., Vercruysse, J. A Comparison of the Sensitivity and Fecal Egg Counts of the McMaster Egg Counting and Kato-Katz Thick Smear Methods for Soil-Transmitted Helminths. PLoS Neglected Tropical Diseases, 2011, 5(6). doi:10.1371/journal.pntd.0001201
Habtamu, K., Degarege, A., Ye-Ebiyo, Y. and Erko, B. Comparison of the Kato-Katz and FLOTAC techniques for the diagnosis of soil-transmitted helminth infections. Parasitology International, 2011, 60(4), 398-402.
Nikolay, B., Brooker, S. and Pullan, R. Sensitivity of diagnostic tests for human soil-transmitted helminth infections: a meta-analysis in the absence of a true gold standard. International Journal for Parasitology, 2014, 44(11), 765-774.
Pusponegoro, H.D., Wirya, I.G.N., Pudjiadi, A.H., Bisanto, J., Zulkarnain, S.Z. Uji diagnostik. 2014.
In: Sastroasmoro S, Ismael S, ed. Dasar Dasar Metodologi Klinis. 5th ed. Jakarta: Sagung Seto. hlm. 219-244
Akobeng, A. Understanding diagnostic tests 1: sensitivity, specificity and predictive values. Acta Paediatrica, 2007, 96(3), pp.338-341.
Deeks, J. Diagnostic tests 4: likelihood ratios. BMJ, 2004, 329(7458), pp.168-169.
Grimes, D. and Schulz, K. Refining clinical diagnosis with likelihood ratios. The Lancet, 365(9469), 2005, 1500-150
Copyright (c) 2023 Indonesian Journal of Tropical and Infectious Disease
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The Indonesian Journal of Tropical and Infectious Disease (IJTID) is a scientific peer-reviewed journal freely available to be accessed, downloaded, and used for research. All articles published in the IJTID are licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which is under the following terms:
Attribution ” You must give appropriate credit, link to the license, and indicate if changes were made. You may do so reasonably, but not in any way that suggests the licensor endorses you or your use.
NonCommercial ” You may not use the material for commercial purposes.
ShareAlike ” If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
No additional restrictions ” You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
