BLASTOCYSTIS AND OTHER INTESTINAL PARASITES INFECTIONS IN ELEMENTARY SCHOOL CHILDREN IN DUKUH VILLAGE, KARANGASEM DISTRICT, BALI

Ni Luh Putu Eka Diarthini, I Kadek Swastika, Luh Ariwati, Rahmadany Isyaputri, Moh. Yasin Fitri N, Sri Hidajati, Sukmawati Basuki

= http://dx.doi.org/10.20473/ijtid.v7i3.7323
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Abstract


Blastocystis spp. is the most common enteric parasitic infection found in several community surveys from developing countries. Blastocystis infections may cause gastrointestinal symptoms, but also cause extraintestinal symptoms such as urticaria and joint pain. Blastocystis infection can also be asymptomatic or a carrier. However, the prevalence of Blastocystis infection in children has not yet been fully investigated in Indonesia, particularly in Bali Province. This study aimed to determine the prevalence of Blastocystis and other intestinal parasites in elementary school children stools in Dukuh village, Karangasem regency. A cross sectional study was conducted in September 2016. A total of 103 school children stools were collected by informed consent and parasites were examined by microscopy with wet mounts method using Lugol’s iodine solution. Thirty-five school children were infected with Blastocystis spp. (35/103, 34%) that consisted of a single infection (29/35, 82.9%) and mix infection with other parasites (6/35, 17.1%). The mix infections were Blastocystis spp. and hookworm infection (1/6, 16.7%), Blastocystis spp. and Entamoeba coli (1/6, 16.7%), Blastocystis spp. and Giardia lamblia (2/6, 33.3%), Blastocystis spp. and Entamoeba histolytica/ Entamoeba dispar (1/6, 16.7%) and Blastocystis spp. and Entamoeba histolytica/ Entamoeba dispar and Giardia lamblia (1/6, 16.7%). The vacuolar forms of Blastocystis were dominantly found, in which was non-infectious form, whereas the infectious form is the cyst form and Blastocystis density was observed less than 5 cells per field of view at 400 magnification in all cases. This study concluded that the high prevalence of Blastocystis infection in elementary school children in Dukuh Village, Karangasem District, Bali that were dominantly single infections and several mix infections with other intestinal parasites. The high prevalence of Blastocystis infection in elementary school children suggested that it needs proper prevention measures for the children in this study area.


Keywords


Blastocystis spp., intestinal parasites, prevalence, children, Bali

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REFERENCES

Farthing M, Salem M, Lindberg G, Dite P, Khalif I, Salazer-Lindo E, et al. Acute diarrhea in adults and children: a global perspective. World Gastroenterol Organ. 2012;(February): 1–24.

Subagyo B, Santoso N. Buku Ajar Gastro-Enterologi-Hepatologi. 1 st ed. Jakarta: IDAI; 2012. 87-102 p.

Nofita E, Harminarti N, Rusjdi SR. Identifikasi Blastocystis Hominis secara Mikroskopis dan PCR pada Sampel Feses di Laboratorium RSUP. Dr. M. Djamil Padang. Maj Kedokt Andalas. 2014; 37(1): 26–31.

Rebolla MF, Silva EM, Gomes JF, Falcão AX. High prevalence of Blastocystis spp. infection in children and staff members attending public urban schools in Sao Paulo State, Brazil. Rev Inst Med Trop Sao Paulo. 2016;(2).

Wawrzyniak I, Poirier P, Texier C, Delbac F, Viscogliosi E, Dionigia M, et al. Blastocystis, an unrecognized parasite: An overview of pathogenesis and diagnosis. Ther Adv Infect Dis. 2013;1(5): 167 78.

Tan KSW. New Insights on Classification, Identification, and Clinical Relevance of Blastocystis spp. 2008; 21(4): 639–65.

Abdulsalam AM, Ithoi I, Al-Mekhlafi HM, Al-Mekhlafi AM, Ahmed A, Surin J. Subtype distribution of Blastocystis isolates in Sebha, Libya. PLoS One. 2013; 8(12): 1–11.

Stensvold C, Arendrup M, Jespersgaard C, Molbak K, Nielsen H. Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study. Diagnostic Microbiol Infect Disesase. 2007; 59: 303–7.

Yoshikawa H, Tokoro M, Nagamoto T, Arayama S, Asih PBS, Rozi IE, et al. Molecular survey of Blastocystis sp. from humans and associated animals in an Indonesian community with poor hygiene.

Parasitol Int. 2016; 65(6): 780–4.

Dogruman-al F, Kustimur S, Yoshikawa H, Tuncer C, Simsek Z. Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey. Mem Inst Oswaldo Cruz, Rio Jaeniro. 2009;104(August): 724–7.

Popruk S, Pintong A, Radomyos P. Diversity of Blastocystis Subtypes in Humans. J Trop Med Parasitol. 2013; 36(2): 88–97.

Eassa SM, Ali HS, Masry SA El, El-fattah AHA. iMedPub Journals Blastocystis hominis among Immunocompromised and Immunocompetent Children in Alexandria, Egypt Abstract. 2016; 1–7.

Daya Pertiwi Foundation. Selayang Pandang Proyek 2014/2015: Pulau Nusa Penida & Kubu Kecamatan Karangasem, Bali. 2015.

WHO. Manual of Basic Technique for a Health Laboratory. 2003.

Leelayoova S, Rangsin R, Taamasri P, Naaglor T, Thathaisong U, Mungthin M. Evidence of waterborne transmission of Blastocystis hominis. Am J Trop Med Hyg. 2004; 70(6): 658–62.

Leelayoova S, Siripattanapipong S, Thathaisong U, Naaglor T, Taamasri P, Piyaraj P, et al. Drinking water: A possible source of blastocystis spp. subtype 1 infection in schoolchildren of a rural community in central Thailand. Am J Trop Med Hyg. 2008; 79(3): 401–6.

Kline K, McCarthy JS, Pearson M, Loukas A, Hotez PJ. Neglected Tropical Diseases of Oceania: Review of Their Prevalence, Distribution, and Opportunities for Control. PLoS Negl Trop Dis. 2013; 7(1): 1–9.

Hotez PJ, Broker S, Bethony JM, Bottazi ME, Loukas A, Xiao S. Hookworm infection. N Engl J Med. 2004; 2: 799–807.

Gardner TB, Hill DR. Treatment of giardiasis. Clin Microbiol Rev. 2001; 14(1): 114–28.

Bedell DA, Garrett RE. Giardidasis. seventh. Rakel RE, editor. Elsevier; 2007. 477–479 p.

MH FH, Karamkhani A, R FH. Waterborne Parasites : A Recent Status of Occurrence, Source and Human Intestinal Parasites in Sources and Tap. 2016;1(61): 17–21.

Kaya S, Çetİn ES, Aridoğan BCi, Arikan S, Demİrcİ M. Pathogenicity of Blastocystis hominis, A Clinical Reevaluation. Turkiye Parazitoloji Derg. 2007; 31(3): 184–7.

Moghaddam D, Ghadirian E, Azami M. Blastocystis hominis and the evaluation of efficacy of metronidazole and trimethoprim/ sulfamethoxazole. Parasito Res. 2005;96 (4): 273–5.

Andersen LOB. Blastocystis in Health and Disease Are We Moving from a Clinical to a Public Health Perspective? PubMed Commons. J Clin Microbiol. 2016; 54(3): 524/8.

Coyle CM, Varughese J, Weiss LM, Tanowitz HB. Blastocystis : To Treat or Not to Treat. 2012; 54: 105–10.

Vassalos CM, Spanakos G, E V, C P, N V. Differences in Clinical Significance and Morphologic Features of Blastocystis sp Subtype 3. 2010; 251–8.

Tan TC, Suresh KG, Smith H V. Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. Parasitol Res. 2008; 104(1): 85–93.

Vdovenko AA. Blastocystis hominis: origin and significance of vacuolar and granular forms. Parasitol Res. 2000;86(1): 8–10.


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