EFFECTIVENESS OF MENIRAN (PHYLLANTHUS NIRURI LINN) AS ANTIBACTERIAL FOR RESISTANCE ANTIBIOTICS PREVENTION OF ENTEROTOXIN ESCHERICHIA COLI
Versions
- 2024-03-18 (2)
- 2018-07-04 (1)
Downloads
Escherichia coli (E. coli) can be isolated from the environment both inside and outside the hospes body. There were 89 serotypes in which showed 21% resistance to various antibiotics, such as enterotoxigenic E.coli. Alternative efforts are needed to overcome this, one of them through the use of medicinal plants, such as meniran (Phyllanthus niruri Linn). Meniran plant is an immunomodulator that serves to repair the immune system of the body. The aim of research is the research was done through several stages: isolation and identification of enterotoxigenic E. coli from several broiler farms in East Java using the polymerase chain reaction (PCR) method, E. coli resistance test against some antibiotics, making meniran extract and activation test against enterotoxigenic E. coli n The study was divided into five treatments: T0+ (group of chickens were infected by enterotoxigenic E. coli T0- (control group, not infected), T1 (infected by enteroxigenic E. coli + 20% meniran extract), T2 (infected by enterotoxigenic E. coli + 25% extract meniran), T3 (infected by enterotoxigenic E. coli + 30% extract meniran). Data were analyzed by ANOVA (Analysis of Variance). The results show that all of E. coli DNA isolates which tested by the PCR method show positive reactions at 600 bp. The next stage, the enterotoxigenic E. coli are resistant to some antibiotics, such as amoxicillin, ampicillin, erythromycin, cephalosporins, tetracycline, cloxacillin and gentamicin. Furthermore, the 30% Phyllanthus niruri Linn extract is effective as an antibacterial for antibiotic resistance enterotoxigenic E. coli. next necessary to write that: The 30% meniran extract is potential for kill of enterotoxigenic E. coli
Review M. Salmonellosis - a Potential Threat To Poultry : J Cell Tissue Res. 2015;15(3):5209–13.
He, X., Qi, W., Quiñones, B., McMahon, S., Cooley, M., and Mandrell RE. Comparison of the Specificities and Efficacies of Primers for Aromatic Dioxygenase Gene Analysis of Environmental Samples. Appl Env Microbiol. 2011;77(11):3551–3557.
Bergeron CR, Prussing C, Boerlin P, Daignault D, Dutil L, Reid-smith RJ, et al. Chicken as Reservoir for Extraintestinal Pathogenic Escherichia coli in Humans , Canada. 2012;18(3):415–21.
Matin MA, Islam MA, Khatun MM. Prevalence of colibacillosis in chickens in greater Mymensingh district of Bangladesh. Vet World. 2017 Jan;10(1):29–33.
Beckson D. B BBL Prepared Tubed Medium for Detection of Fecal Coliform Bacteria. 2011;8806881.
Trivedi RN, Akhtar P, Meade J, Bartlow P, Ataai MM, Khan SA, et al. High-Level Production of Plasmid DNA by Escherichia coli DH5α Ω sacB by Introducing inc Mutations. Parales RE, editor. Appl Environ Microbiol. 2014 Dec 1;80(23):7154–60.
Yuniati Y, Rollando R. Isolation of antibacterial compounds from endophyte fungal of fusarium Isolation of antibacterial compounds from endophyte fungal of fusarium sp . In phyllanthus niruri linn . Leaves. 2018;(March).
Permata DA, Murtius WS, August J. Research Journal of Pharmaceutical , Biological and Chemical Sciences Alpha Amylase Inhibition and Antioxidant Activity of Phyllanthus niruri Powder Drink . 7(656):656–62.
Aldi Y, Rasyadi Y, Handayani D. Immunomodulatory Activity of Meniran Extracts (Phyllanthus niruri Linn.) on Broiler Chickens. J Sains Farm Klin. 2014;1(1):20–6.
Prasetyo RH, Safitri E. Effects of honey to mobilize endogenous stem cells in efforts intestinal and ovarian tissue regeneration in rats with protein energy malnutrition. Asian Pacific J Reprod. 2016 May;5(3):198–203.
Prasetyo RH, Hestianah EP. Honey can repairing damage of liver tissue due to protein energy malnutrition through induction of endogenous stem cells. Vet world. 2017 Jun;10(6):711–5.
Safitri E, Utama S, Widiyatno TV, Sandhika W, Prasetyo RH. Auto-regeneration of mice testicle seminiferous tubules due to malnutrition based on stem cells mobilization using honey. Asian Pacific J Reprod. 2016 Mar;5(1):31–5.
Safitri E, Widiyatno T V., Prasetyo RH. Honeybee product therapeutic as stem cells homing for ovary failure. Vet World. 2016 Nov;9(11):1324–30.
Samik A, Safitri E. Mycotoxin binders potential on histological of ovary mice exposed by zearalenone. Vet World. 2017 Mar;10(3):353–7.
Dandjesso C, Klotoé JR, Dougnon T V., Sègbo J, Atègbo JM, Gbaguidi F, et al. Phytochemistry and hemostatic properties of some medicinal plants sold as anti-hemorrhagic in Cotonou Markets (Benin). Indian J Sci Technol. 2012;5(8):3105–9.
Maddox CE, Laur LM, Tian L. Antibacterial Activity of Phenolic Compounds Against the Phytopathogen Xylella fastidiosa. Curr Microbiol. 2010 Jan 8;60(1):53–8.
Jawetz, Melnick and A. Medical Microbiology. 6th Ed. New York: A LANGA medial book; 2013.
Samik A, Safitri E. Potency of mycotoxin binders on MDA level, expressions of caspase 9 and caspase 3 in the uterus of mice exposed to zearalenone. Iraqi J Vet Sci. 2017;31(1):29–33.
Lluque, A., Mercado, E., Riveros, M., Alvarado , L., Carlos, E., Colichón, A., Salazar, E., Ochoa T. Comparison of enteropathogenic Escherichia coli (EPEC) diagnosis by serology and by polymerase chain reaction (PCR). Rev Gastoenterol Peru. 2010;30(2):121–5.
Lara VM, Carregaro AB, Santurio DF, Sá MF de, Santurio JM, Alves SH. Antimicrobial Susceptibility of Escherichia coli Strains Isolated from Alouatta spp. Feces to Essential Oils. Evidence-Based Complement Altern Med. 2016;2016:1–4.
Rumagit, B.I., Banne, Y., Dilampudi Y. Preparation of ulcer ointment from meniran (Phyllanthus niruri L.) herb extract. J STIFA Makassar. 2015;1(1):15–9.
Landers TF. A Review of Antibiotic Use in Food Animals : Perspective , Policy , and Potential. 127(February 2012).
Katouli M. Population structure of gut Escherichia coli and its role in development of extra-intestinal infections. 2010;2(2):59–72.
Willey, J.M., Sherwood, L.M., Woolverten CJ. Prescott's Principle of Microbiology. New York: McGraw-Hill Higher Education; 2009. 532-567 p.
Munita JM, Arias CA, Unit AR, Santiago A De. HHS Public Access. 2016;4(2):1–37.
Cushnie TPT, Cushnie B, Lamb AJ. International Journal of Antimicrobial Agents Alkaloids : An overview of their antibacterial , antibiotic-enhancing and antivirulence activities. Int J Antimicrob Agents. 2014;44(5):377–86.
Copyright (c) 2018 Indonesian Journal of Tropical and Infectious Disease
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The Indonesian Journal of Tropical and Infectious Disease (IJTID) is a scientific peer-reviewed journal freely available to be accessed, downloaded, and used for research. All articles published in the IJTID are licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which is under the following terms:
Attribution ” You must give appropriate credit, link to the license, and indicate if changes were made. You may do so reasonably, but not in any way that suggests the licensor endorses you or your use.
NonCommercial ” You may not use the material for commercial purposes.
ShareAlike ” If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
No additional restrictions ” You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
