Antibacterial Activity of Ethanol Extract of Kemuning (Murraya Paniculata) Against Klebsiella pneumoniae ESBL by In Vitro Test
Downloads
Klebsiella pneumoniae Extended-spectrum β-lactamase (ESBL) was one of the microorganism that cause nosocomial infection which resistant to beta-lactams antibiotics. Orange Jessamine (Murraya paniculata) was traditional medicine which believed has antibacterial components, such as: fl avonoids, alkaloids, essential oils, coumarins, terpenoids, tannins, and saponins. In the previous studies, there was antibacterial activity in ethanolic extract of Murraya paniculata againsts E.coli, K.pneumoniae, S.typhi, E.faecalis, P.aeruginosa, S.fl exneri, S.aureus, and S.sonneii with concentration 200 mg/ mL. There has not experiment about ethanolic extract of Murraya paniculata against Klebsiella pneumoniae ESBL yet. The aim of this study was to fi nd out the in vitro antibacterial activity of ethanol extracts of Murraya Paniculata against Klebsiella pneumoniae ESBL Broth dilution method with concentration 200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL, 12,5 mg/mL, 6,25 mg/mL, and 3,125 mg/mL were used for the determination of the Minimal Inhibitory Concentration (MIC). While the Minimal Bacterial Concentration (MBC) was assessed using streaking method in Nutrient Agar Plate. The highest concentration in this study was obtained from 100 g of Murraya paniculata leaves dissolved in 500 mL of 40% ethanol. The study was carried out 4 times replication. At the time of the sterility test extract, germ growth appeared on Nutrient Agar Plate media, so the extract was fi ltered before being used for research. After incubation at 37 °C for 24 hours, growth of bacterial colonies on all agar plates was observed. The concentration of the ethanol extract of Murraya Paniculata (200 mg/mL) did not inhibit the growth of Klebsiella pneumoniae ESBL. The ethanol extracts of Murraya paniculata in concentration 200 mg/mL had no antibacterial activity against Klebsiella pneumoniae ESBL.
Jeyamohan D. Angka Prevalensi Infeksi Nosokomial Pada Pasien Luka Operasi Pasca Bedah Di Bagian Bedah Di Rumah Sakit Umum Pusat Haji Adam Malik, Medan Dari Bulan April Sampai September 2010. Microbiology and Management of Hospital Infection 2011.
Vincent JL. Nosocomial infections in adult intensive-care units. Lancet. 2003;361(9374):2068-77.
Kuntaman. Analysis of Microbiology Results for Managing Hospital Acquired Infection Effectively Surabaya: Universitas Airlangga; 2011 [cited 2014 27th May]. Available from: http://kuntaman-fk.web.unair.ac.id/artikel_detail-35548-Umum-Microbiology%20and%20Management%20of%20Hospital%20Infection.html.
Chung DR, Song JH, Kim SH, Thamlikitkul V, Huang SG, Wang H, et al. High prevalence of multidrug-resistant nonfermenters in hospital-acquired pneumonia in Asia. American journal of respiratory and critical care medicine. 2011;184(12):1409-17.
Munoz-Price LS, Jacoby GA. Extended-spectrum betalactamases 2014 [cited 2014 9th August ]. Available from: http://www.uptodate.com/contents/extended-spectrum-beta-lactamases.
Paterson DL, Ko WC, Von Gottberg A, Mohapatra S, Casellas JM, Goossens H, et al. Antibiotic therapy for Klebsiella pneumoniae bacteremia: implications of production of extended-spectrum beta-lactamases. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2004;39(1):31-7.
Tsai SS, Huang JC, Chen ST, Sun JH, Wang CC, Lin SF, et al. Characteristics of Klebsiella pneumoniae bacteremia in community-acquired and nosocomial infections in diabetic patients. Chang Gung medical journal. 2010;33(5):532-9.
Huang WY, Cai YZ, Zhang Y. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutrition and cancer. 2010;62(1):1-20.
Syahadat RM, Aziz SA. Pengaruh Komposisi Media Dan Fertigasi Pupuk Organik Terhadap Kandungan Bioaktif Daun Tanaman Kemuning (Murraya paniculata (L.) Jack) Di Pembibitan. 2012.
Ng MK, al e. Bioactivity studies and chemical constituents of Murraya paniculata (Linn) Jack. International Food Research Journal. 2012;19(4):1307-12.
Matos MJ, Vazquez-Rodriguez S, Santana L, Uriarte E, Fuentes-Edfuf C, Santos Y, et al. Looking for new targets: simple coumarins as antibacterial agents. Medicinal chemistry. 2012;8(6):1140-5.
Kawase M, Varu B, Shah A, Motohashi N, Tani S, Saito S, et al. Antimicrobial activity of new coumarin derivatives. Arzneimittel-Forschung. 2001;51(1):67-71.
Xiang JL. Kamuning 2013 [cited 2014 1st Jun ]. Available from: http://stuartxchange.com/Kamuning.html.
Rao S. extendedspectrum beta-lactamases2015.
Paterson DL, Bonomo RA. Extended-spectrum beta-lactamases: a clinical update. Clinical microbiology reviews. 2005;18(4):657-86.
Rupp ME, Fey PD. Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae: considerations for diagnosis, prevention and drug treatment. Drugs. 2003;63(4):353-65.
Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clinical microbiology reviews. 2001;14(4):933-51, table of contents.
Iswantini D, al e. Zingiber cassumunar, Guazuma ulmifolia, and Murraya paniculata Extracts as Antiobesity: In Vitro Inhibitory Effect on Pancreatic Lipase Activity. Hayati Journal of Biosciences. 2011;18(1):6-10.
Harborne JB. Phytochemistry. London: Academic Press; 1993.
Parwata IM, Oka A, Dewi PFS. Isolasi dan Uji Aktivitas Antibakteri Minyak Atsiri dari Rimpang Lengkuas (Alpinia galanga L.). Jurnal Kimia. 2008;2(2):100-4.
Dwidjoseputro D. Dasar-Dasar Mikrobiologi. Jakarta: Djambatan; 1994.
Rahayu MD. Uji Efektivitas Antibakteri Ekstrak Lengkuas Merah (Alpinia purpurata K. Schum) terhadap Bakteri Escherichia coli secara In Vitro2012.
Robinson T. Kandungan Organik Tumbuhan Tingkat Tinggi. Bandung: Institut Teknologi Bandung; 1991.
Juliantina FR, Citra DA, Nirwani B, Nyrmasitoh T, Bowo ET. Manfaat Sirih Merah (Piper crocatum) sebagai Agent Anti bakterial terhadap Bakteri Gram Positif dan Bakteri Gram Negatif. Jurnal Kedokteran dan Kesehatan Indonesia. 2009:10-9.
Kayser O, Kolodziej H. Antibacterial activity of simple coumarins:structural requirements of biological activity. Z Naturforsch. 1999;54c:169-17.
Epifano F, Genovese S, Menghini L, Curini M. Chemistry and pharmacology of oxyprenylated secondary plant metabolites. Phytochemistry. 2007;68(7):939-53.
Banwart GJ. Basic Food Microbiology. New York: AVI; 1981.
Baumann E, Stoya G, Volkner A, Richter W, Lemke C, Linss W. Hemolysis of human erythrocytes with saponin affects the membrane structure. Acta histochemica. 2000;102(1):21-35.
Segal R, Shatkovsky P, Milo-Goldzweig I. On the mechanism of saponin hemolysis--I. Hydrolysis of the glycosidic bond. Biochemical pharmacology. 1974;23(5):973-81.
Winter WP. Mechanism of saponin induced red cell hemolysis: evidence for the involvement of aquaporin CHIP28. Blood. 1994;84:445.
Ya C, al e. Carbohydrate — Polyphenol Complexation. Chemistry and Significance of Condensed Tannins. 1989:307-22.
Gautam MK, al e. In-vitro antibacterial activity on human pathogens and total phenolic, flavonoid contents of Murraya paniculata Linn. Leaves. Asian Pacific journal of tropical biomedicine. 2012:1660-3.
Sundaram M, al e. Studies on in vitro Antibacterial, Antifungal Property and Antioxidant Potency of Murraya paniculata. Pakistan Journal of Nutrition. 2011;10(10):925-9.
Lin RD, Chin YP, Lee MH. Antimicrobial activity of antibiotics in combination with natural flavonoids against clinical extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae. Phytotherapy research : PTR. 2005;19(7):612-7.
Zhou XZ, Jia F, Liu XM, Yang C, Zhao L, Wang YJ. Total alkaloids from Sophora alopecuroides L. increase susceptibility of extended-spectrum beta-lactamases producing Escherichia coli isolates to cefotaxime and ceftazidime. Chinese journal of integrative medicine. 2013;19(12):945-52.
Kinoshita T, Firman K. Highly oxygenated flavonoids from Murraya paniculata. Phytochemistry. 1996;42:1207-10.
Safdari H, Neshani A, Sadeghian A, Ebrahimi M, Iranshahi M, Sadeghian H. Potent and selective inhibitors of class A beta-lactamase: 7-prenyloxy coumarins. The Journal of antibiotics. 2014;67(5):373-7.
N’guessan JD, al e. Antibacterial activity of the aqueous extract of Thonningia sanguinea against Extended-Spectrum-β-Lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae strains. Tropical Journal of Pharmaceutical Research. 2007;6(3):779-83.
Orhan DD, Ozcelik B, Ozgen S, Ergun F. Antibacterial, antifungal, and antiviral activities of some flavonoids. Microbiological research. 2010;165(6):496-504.
Copyright (c) 2021 Indonesian Journal of Tropical and Infectious Disease
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The Indonesian Journal of Tropical and Infectious Disease (IJTID) is a scientific peer-reviewed journal freely available to be accessed, downloaded, and used for research. All articles published in the IJTID are licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which is under the following terms:
Attribution — You must give appropriate credit, link to the license, and indicate if changes were made. You may do so reasonably, but not in any way that suggests the licensor endorses you or your use.
NonCommercial — You may not use the material for commercial purposes.
ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
