Preleminary Study: The Effects of Different Cryoprotectants on Damage and Hatching Rates of African Catfish (Clarias gariepinus)

Akhmad Taufiq Mukti; Septuresty Hartri Eka; Woro Hastuti Satyantini; Ahmad Shofy Mubarak

doi:10.20473/jafh.v9i3.16562

Authors

Akhmad Taufiq Mukti
atm.mlg@gmail.com
Departemen Manajemen Kesehatan Ikan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, Indonesia, Indonesia
Septuresty Hartri Eka Program Studi Bioteknologi Perikanan dan Kelautan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, Indonesia, Indonesia
Woro Hastuti Satyantini Departemen Manajemen Kesehatan Ikan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, Indonesia, Indonesia
Ahmad Shofy Mubarak Departemen Kelautan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, Indonesia, Indonesia

Vol. 9 No. 3 (2020): JAFH Vol. 9 No. 3 September 2020

Articles

Downloads

PDF

Abstract
How to Cite
Metrics
References
License

This study was aimed to observe the effects of different cryoprotectants in embryo cryopreservation on damage and hatching rates of African catfish (Clarias gariepinus) embryo. The gastrula-staged embryos were treated 5% (v/v) solutions concentration of dimethyl sulfoxide (DMSO), propylene glycol (PG), honey, and combined cryoprotectants, respectively and preserved at temperatures of 4 and 0ºC for 30 min, 1 h, 2 h, 3 h, 4 h, 5 h, and 6 h, respectively. Thawing of embryos was conducted in freshwater at a temperature of 28ºC. Then, the embryos were incubated in the aquaria at 28ºC temperature. The result showed that the different cryoprotectants in gastrula-staged embryo cryopreservation affect damage and hatching rates of African catfish embryos at different length of preservation. The percentage of catfish embryo damage increases with different length of preservation and the storage temperature. A combination of DMSO-honey and PG-honey has the lowest damage percentage and the highest hatching rate of catfish embryo compared to other treatments (p<0.05).

Mukti, A. T., Hartri Eka, S., Hastuti Satyantini, W., & Shofy Mubarak, A. (2020). Preleminary Study: The Effects of Different Cryoprotectants on Damage and Hatching Rates of African Catfish (Clarias gariepinus). Journal of Aquaculture and Fish Health, 9(3), 214–221. https://doi.org/10.20473/jafh.v9i3.16562

Download Citation

Ahammad, M.M., Bhattacharyya, D. dan Jana, B.B., 2002. The hatching of common carp Cyprinus carpio L. embryos in response to exposure to different concentrations of cryoprotectant at low temperatures. Cryobiology, 44, pp. 114-121. https://doi.org/10.1016/s0011-2240(02)00012-3.

Ahammad, M.M., Bhattacharyya, D. dan Jana, B.B., 2003. Stage-dependent hatching responses of rohu Labeo rohita embryos to different concentrations of cryoprotectants and temperatures. Cryobiology, 46, pp. 2-16. https://doi.org/10.1016/ s0011-2240(02)00138-4.

Ball, D.W., 2007. The chemical composition of honey. Journal of Chemical Education, 84(10), pp. 43-46. https://doi.org/10.1021/ed084 p1643.

Beirao, J., Robles, V., Herraez, M.P., Sarasquete, C., Dinis, M.T. dan Cabrita, E., 2006. Cryoprotectant microinjection toxicity and chilling sensitivity in gilthead seabream (Sparus aurata) embryos. Aquaculture, 261(3), pp.897-903. https://doi.org/10.1016/j.aquaculture.2006.07.039

Bhattacharya, S. dan Prajapati, B.G., 2016. A Review on cryoprotectant and its modern implication in cryonics. Asian Journal of Pharmaceutics, 10 (3), pp. 154-159. https://dx.doi.org /10.22377/ajp.v10i3.721.

Chao, N.H. dan Liao, I.C., 2001. Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture, 197, pp. 161-189. https://doi.org/10.1016/B978-0-444-50913-0.50011-4.

Dasaria, M.A., Kiatsimkula, P.P., Sutterlin, W.R. dan Suppesa, G.J., 2005. Low-pressure hydrogenolysis of glycerol to propylene glycol. Applied Catalysis, 281, pp. 225-231. https:// doi.org/10.1016/j.apcata.2004.11.033.

Eka, S.H., Mukti, A.T., Satyantini, W.H. dan Mubarak, A.S., 2020. Preliminary study: the effect of cryopreservation on the gastrula-staged embryo of African catfish (Clarias gariepinus). IOP Conference Series: Earth and Environmental Science, (inpres). https://iopscience. iop.org/article/10.1088/1755-1315 /441/1/012124.

Fornari, D.C., Ribeiro, R.P., Streit Jr., D.P., Godoy, LC., Neves, P.R., Oliveira, D.D. dan Sirol, R.N., 2011. Effect of cryoprotectants on the survival of cascudo preto (Rhinelepis aspera) embryos stored at -8oC. Zygote, 22, pp. 58-63. https://doi.org/10.1017/ S0967199411000517.

Fornari, D.C., Ribeiro, R.P., Streit Jr., D.P., Vargas, L., Godoy, L.C., Oliveira, C.AL, Digmayer, M., Galo, J.M. dan Neves, P.R., 2012. Increasing storage capability of pacu (Piaractus mesopotamicus) embryos by chilling: development of a useful methodology for hatcheries management. CryoLetters, 33, pp. 125-134. https://pubmed.ncbi.nlm. nih.gov/22576116/.

Hagedorn, M., Kleinhans, F.W., Artemov, D. dan Pilatus, U., 1998. Characterization of a major permeability barrier in the zebrafish embryo. Biology of Reproduction, 59, pp. 40-50. https://doi.org/10.1095/ biolreprod59.5.1240.

Kais, B., Schneider, K.E., Keiter, S., Henn, K., Ackermann, C. dan Braunbeck, T., 2013. DMSO modifies the permeability of the zebrafish (Andio rerio) chorion-implications for the fish embryo test (FET). Aquatic Toxicology, 33, pp. 229-238. https:// doi.org/10.1016/j.aquatox.2013.05.022.

Keivanloo, S., Mohammad, S. dan Hajibeglou, A., 2017. Effect of vitrification by propylene glycol-based solutions on the survival rate of the persian sturgeon Acipenser persicus embryos. Turkish Journal of Fisheries and Aquatic Sciences, 17, pp. 681-687. https://doi.org/10. 4194/1303-2712-v17_4_04.

Lahnsteiner, F., 2009. Factors affecting storage of zebrafish (Danio rerio) embryos. Theriogenology, 72, pp. 333-340. https://doi.org/10.1016/ j.theriogenology.2009.03.003.

Lin, C., Spikings, S., Zhang, T. dan Rawson, D.M., 2009a. Effect of chilling and cryopreservation on expression of pax genes in zebrafish Andio rerio embryos and blastomeres. Cryobiology, 59, pp. 42-47. https://doi.org/10.1016/j. cryobiol.2009.04.007.

Lin, C., Zhang, T. dan Rawson, D.M., 2009b. Cryopreservation of zebrafish Andio rerio blastomeres by controlled slow cooling. Cryoletters, 30(2), pp. 132-141. https://pub med.ncbi.nlm.nih.gov/19448862/.

Lopes, T.D.S., Sanches, E.A., Okawara, R.Y.O. dan Romagosa, E., 2015. Chilling of Steindachneridion parahybae, Siluriformes: pimelo-didae embryos. Theriogenology, 84, pp. 538-544.https://doi.org/10. 1016/j.vas.2019.100046.

Mukti, A.T., Mubarak, A.S. dan Wahyurini, E.T., 2019. Aplikasi teknologi induced spawning untuk mempercepat pemijahan ikan lele pada mitra program kemitraan masyarakat. Journal of Aquaculture and Fish Health, 8(1), pp. 46-53. http://dx.doi.org/10.20473/jafh.v8i1.12004.

Shaluei, F., Imanpoor, M.R., Shabani, A. dan Esfahani, N.M.H., 2013. Effect of different concentrations of permeable and non-permeable cryoprotectants on the hatching rate of goldfish Carassius auratus embryos. Asian Pacific Journal of Reproduction, 2(3), pp. 185-188. https://doi.org/10.1016/s2305-0500(13)60144-x.

Sharifuddin, M.M. dan Azizah, M.N.S., 2014. Preliminary studies on cryopreservation of snakehead Channa striata embryos. Cryobiology, 69, pp. 1-9. https://doi. org/10.1016/j.cryobiol.2014.04.001.

Sheshtawy, E.R.I., Badry, E.D.A., Sisy, E.G.A., Nattat, E.W.S. dan Almaty, A.M.A., 2016. Natural honey as a cryoprotectant to improve Arab stallion post-thawing sperm parameters. Asian Pacific Journal of Reproduction, 5(4), pp. 331-334. http://dx.doi.org/10.1016%2Fj.apjr.2016.06.004.

Tian, Y., Jiang, J., Song, L., Chen, Z., Zhai, J., Liu, J., Wang, N. dan Chen, S., 2015. Effects of cryopreservation on the survival rate of the seven-band grouper Epinephelus septemfasciatus embryos. Cryobiology, 71, pp. 499-506. https://doi.org/10.1016/j. cryobiol.2015.10.147.

Xiao, Z.Z., Zhang, L.L., Xu, X.Z., Liu, Q.H., Li, J., Ma, D.Y., Xu, S.H., Xue, Y.P. dan Xue, Q.Z., 2008. Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos. Theriogenology, 70, pp. 86-92. https://doi.org/10.1016/j.the riogenology.2008.06.028.

Zhang, Y.Z., Zhang, S.C., Liu, X.Z., Xu, Y.J., Hu, J.H., Xu, Y.Y., Li, J. dan Chen, S.L., 2005. Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos. Theriogenology, 63, pp. 763-773. https://doi.org/10.1016/j.theriogenology.2004.04.011.

Preleminary Study: The Effects of Different Cryoprotectants on Damage and Hatching Rates of African Catfish (Clarias gariepinus)

Authors

Downloads

Most read articles by the same author(s)

Login

Accreditation Certificate

Journal Policy

EditorialTeam

Meet Our Editorial Team

Instruction For Author

Article Template

Reference Manager

Grammar Editor

Back Issue JAFH

This Journal is Supported by

IndexedBy

Indexing

Stats

Stats

Address

Contact Info: