Potential of Antioxidant Activity Caulerpa racemosa Extract Using DES Solvent and Different Sonication Times as An Antibacterial Against Pathogenic Bacteria
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Caulerpa racemosa, a type of green algae, has been studied for its potential health benefits, including antioxidant and an- tibacterial properties. The extraction of bioactive compounds from marine algae using green solvents like Deep Eutectic Sol- vents (DES) has gained interest due to their efficiency and en- vironmental friendliness. This research aimed to determine the potential antioxidant activity of C. racemosa extract using DES solvents and different sonication times as an antibacterial against pathogenic bacteria. The research employed a Facto- rial Completely Randomized Design (CRD) with two variables: sonication time (10, 20, and 30 minutes) and different DES solvents (ethanol and ethylene glycol (1:20) with 90% aquades, and methanol and glycerol (1:10) with 90% aquades. The highest antioxidant activity was found in DES Methanol-Glycerol (36.39 mg TE/g dw), followed by DES Eth- anol-Ethylene glycol (28.30 mg TE/g dw), both significantly different (p<0.05). The C. racemosa extract inhibited the growth of V. parahaemolyticus with an inhibition zone of 8.08 mm at 50 ppm and 13.13 mm at 1,000 ppm. For V. harveyi, inhibition started at 6.37 mm at 50 ppm, reaching 11.70 mm at 1,000 ppm. DES Methanol-Glycerol with a 10-minute soni- cation provided the highest antioxidant activity and effectively inhibited V. parahaemolyticus and V. harveyi. Extraction of C. racemosa with DES-MG solvent and varying sonication times influences its antioxidant activity and antibacterial properties.
Abbott, A.P., Capper, G., Davies, D.L., Rasheed, R.K. and Tambyrajah, V., 2003. Novel Solvent Properties of Choline Chloride/Urea Mixtures. Chemical Communications, 2003, pp.70-71. https://doi.org/10.1039/B210714G
Ahmad, I., Yusniah, A., Nur, Y., Prabowo, W.C. and Herman, 2020. Total Poly-phenols Enrichment from Mitragyna speciosa Korth Havil Leaves Using Green Solvent Based Microwave-assisted Extraction Method. Jurnal Farmasi Galenika, 6(2), pp.338-346. https://doi.org/10.22487/j24428744.2020.v6.i2.15035
Belkacemi, L., Belalia, M., Djendara, A.C. and Bouhadda, Y., 2020. Antioxidant and antibacterial activities and identifica-tion of bioactive compounds of vari-ous extracts of Caulerpa racemosa from Algerian coast. Asian Pacific Journal of Tropical Biomedicine, 10(2), pp.87-94. https://doi.org/10.4103/2221-1691.275423
Benítez-Correa, E., Bastías-Montes, J.M., Acuña-Nelson, S. and Muñoz-Fariña, O., 2023. Effect of Choline Chlorid-based Deep Eutectic Solvents on Poly-phenols Extraction from Cocoa (Theo-brama cacao L.) Bean Shells and Anti-oxidant Activity Extracts. Current Re-search in Food Science, 7, 100614. https://doi.org/10.1016/j.crfs.2023.100614
Cameron, D.K. and Wang, Y.J., 2006. Applica-tion of protease and high-intensity ul-trasound in corn starch isolation from degermed corn flour. Cereal Chemis-try, 83(5), pp.505-509. https://doi.org/10.1094/CC-83-0505
Damayanti, D.S., Damayanthi, E., Riyadi, H., Wibawan, I.W.T. and Handharyani, E., 2023. The Analysis of Antioxidant Ca-pacities and Sensory in Sea Grapes (Caulerpa racemose) Powdered Drink as a Therapeutic Obesity. Amerta Nu-trition, 7(2), pp.175-184. https://doi.org/10.20473/amnt.v7i2.2023.175-184
Darsana, I.G.O., Besung, I.N.K. and Mahatmi, H., 2012. Potensi daun binahong (Anredera cordifolia) (Tenore Steensis) dalam menghambat pertumbuhan bakteri Escherechia coli secara in vitro. Indonesia Medicus Veterinus, 1(3), pp.337–351.
Gu, Y. and Jérome, F., 2010. Glycerol as a Sustainable Solvent for Green Chemis-try. Green Chemistry, 12(7), pp.1127-1138. https://doi.org/10.1039/C001628D
Hao, H., Yan, M., He, B., Li, M., Liu, Q., Cai, Y., Zhang, X. and Huang, R., 2019. Chemi-cal composition and immunostimula-tory properties of green alga Caulerpa racemosa var peltata. Journal of Food and Agricultural Immunology, 30(1), pp.937-954. https://doi.org/10.1080/09540105.2019.1646216
Hidayah, N., Mustikaningtyas, D. and Bintari, S.H., 2017. Aktivitas Antibakteri Infusa Simplisia Sargassum muticum ter-hadap Pertumbuhan Staphylococcus aureus. Life Science, 6(2), pp.49-54. https://journal.unnes.ac.id/sju/UnnesJLifeSci/article/view/25345
Hidayati, K., 2020. Daya hambat ekstrak hot water Caulerpa racemosa terhadap Vibrio harveyi dan Vibrio parahaemo-lyticus secara in vitro. Skripsi. Fakultas Perikanan dan Kelautan. Universitas Airlangga. p.42.
Huyyirnah and Fitriyani, 2020. Metode Penyimpanan Bakteri Vibrio algin-olitycus dan Vibrio harveyi dalam Me-dia TSB (Tryptic Soy Broth) dan Glise-rol. Integrated Lab Journal, 8(2), pp.91-101. https://ejournal.uin-su-ka.ac.id/pusat/integratedlab/article/view/080208
Jayusri, Cokrowati, N. and Diniarti, N., 2023. Cultivation of Seaweed Caulerpa rac-emose Using Different Substrate on A Laboratory Scale. Asian Journal of Aquatic Sciences, 6(3), pp.441-446. https://doi.org/10.31258/ajoas.6.3.441-446
Kandhasamy, M. and Arunachalam, K.D., 2008. Evaluation of in vitro antibacte-rial property of seaweeds of southeast coast of India. African Journal of Bio-technology. 7(12), pp.1958-1961. https://doi.org/10.5897/AJB08.120
Kuldiloke, J., 2002. Effect of Ultrasound Temperature and Pressure Treath-ments on Enzyme Activity and Quality of Fruit and Vegetable Juices. Berlin: Dissertationder Technischen Universi-tat Berlin.
Kumar, J.G.S., Umamaheswari, S., Kavimani, S. and Ilavarasan, R., 2019. Pharmaco-logical potential of green algae Caulerpa: A review. International Journal Pharmaceutical Sciences and Research, 10(3), pp.1014- 1024. https://doi.org/10.13040/IJPSR.0975-8232.10(3).1014-24
Kurniaji, A., Idris, M. and Muliani, 2020. Uji daya hambat ekstrak daun mangrove (Sonneratia alba) pada bakteri Vibrio harveyi secara in vitro. Jurnal Sains Teknologi Akuakultur, 3(2), pp.84-92.
Latifah, A.T.W., 2015. Prarancangan Pabrik etilen glikol dari etilen glikol dari etilen oksida dan air dengan proses hidrasi non katalik kapasitas 220.000 ton/tahun. Naskah Publikasi Tugas Akhir, Jurusan Teknik Kimia Fakultas Teknik Universitas Muhammadiyah Surakarta. http://eprints.ums.ac.id/id/eprint/36189
Li, P., Huo, L., Su, W., Lu, R., Deng, C., Liu, L., Deng, Y., Guo, N., Lu, C. and He, C., 2004. Free radical-scavenging capaci-ty, antioxidant activity and phenolic content of Pouzolzia zeylanica. Journal of the Serbian Chemical Society, 76(5), pp.709-717. https://doi.org/10.2298/JSC100818063L
Lomartire, S. and Gonçalves, A.M.M., 2023. An Overview on Antimicrobial Poten-tial of Edible Terrestrial Plants and Marine Macroalgae Rhodophyta and Chlorophyta Extracts. Marine Drugs, 21(3), 163. https://doi.org/10.3390/md21030163
Ma’aruf, W.F., Ibrahim, R., Dewi, E.N., Susan-to, E. and Amalia, U., 2013. Caulerpa racemosa and Gracilaria verrucosa Profile as Edible Foods. Saintek Peri-kanan: Indonesian Journal of Fisheries Science and Technology, 9(1), pp.68-74. https://ejournal.undip.ac.id/index.php/saintek/article/view/8114
Mannu, A., Blangetti, M., Baldino, S. and Prandi, C., 2021. Promising Techno-logical and Industrial Applications of Deep Eutectic Systems. Materials, 14(10), 2494. https://doi.org/10.3390/ma14102494
Marraskuranto, E., Nursid, M., Utami, S., Setyaningsih, I. and Tarman, K., 2021. Kandungan Fitokimia, Potensi Anti-bakteri dan Antioksidan Hasil Ekstraksi Caulerpa racemosa dengan Pelarut Berbeda. Jurnal Pascapanen dan Bioteknologi Kelautan dan Peri-kanan, 16(1), pp.1-10. http://dx.doi.org/10.15578/jpbkp.v16i1.696
Mokoginta, T.A., Yudistira, A. and Mpila, D.A., 2021. Uji aktivitas antioksidan ekstrak etanol rumput laut Caulerpa racemosa dari Pulau Mantehage Su-lawesi Utara. Pharmacon, 10(3), pp.948-952. https://doi.org/10.35799/pha.10.2021.35596
Niawanti, H., 2017. Purifikasi Biodiesel Menggunakan Deep Eutectic Solvent. Tesis, Program Magister, Fakultas Teknologi Industri, Institut Teknologi Sepuluh Nopember, Surabaya.
Noack, K., Kiefer, J. and Leipertz, A., 2010. Concentration‐dependent hydrogen‐bonding effects on the dimethyl sul-foxide vibrational structure in the presence of water, methanol, and eth-anol. ChemPhysChem, 11(3), pp.630-637. https://doi.org/10.1002/cphc.200900691
Pagliaro, M. and Rossi, M., 2008. The Future of Glycerol: New Usage of a Versatile Raw Material. RSC Publishing, Cam-bridge.
Palaniyappan, S., Sridhar, A., Kari, Z.A., Tél-lez-Isaías, G. and Ramasamy, T., 2023. Evaluation of Phytochemical Screen-ing, Pigment Content, In Vitro Antioxi-dant, Antibacterial Potential and GC-MS Metabolite Profiling of Green Sea-weed Caulerpa racemosa. Marine Drugs, 21(5), 278. https://doi.org/10.3390/md21050278
Panda, S.K., 2012. Assay guided comparison for enzymatic and non-enzymatic an-tioxidant activities with special refer-ence to medicinal plants. Antioxidant Enzyme, 14(1), pp.382-400. http://dx.doi.org/10.5772/50782
Rosmania and Yuniar, 2021. Pengaruh Wak-tu Penyimpanan Inokulum Escherichia coli dan Staphilococcus aureus Pada Suhu Dingin Terhadap Jumlah Sel Bak-teri di Laboratorium Mikrobiologi. Jurnal Penelitian Sains, 23(3), pp.117-124. https://doi.org/10.56064/jps.v23i3.624
Sander, A., Rogošić, M., Slivar, A. and Žuteg, B., 2016. Separation of hydrocarbons by means of liquid-liquid extraction with deep eutectic solvents. Solvent Extraction and Ion Exchange, 34(1), pp.86-98. https://doi.org/10.1080/07366299.2015.1132060
Singkoh, M.F.O., 2011. Aktivitas antibakteri ekstrak alga laut Caulerpa racemose dari perairan Pulau Nain. Jurnal Peri-kanan dan Kelautan Tropis, 7(3), pp.123-127. https://doi.org/10.35800/jpkt.7.3.2011.189
Sunarni, T., Pramono, S. and Asmah, R., 2007. Flavonoid antioksidan pe-nangkap radikal dari daun kepel (Stelechocarpus burahol (Bl.) Hook f. & Th.). Majalah Farmasi Indonesia, 18(3), pp.111-116. http://download.garuda.kemdikbud.go.id/article.php?article=411045&val=8865&title=Antioxidantfree%20radical%20scavenging%20of%20flavonoid%20from%20The%20Leaves%20of%20Stelechocarpus%20burahol%20Bl%20Hook%20f%20%20Th
Syam, H., Jamaluddin, P., Mustarin, A. and Rivai, A.A., 2018. Social Economic Conditions of Seaweed Farmes in Jeneponto Regency. Advances in Social Science, Education and Humanity Re-search (ASSEHR), 227, pp.590-595. https://doi.org/10.2991/icamr-18.2019.140
Tapotubun, A.M., Matrutty, T.E.A.A., Riry, J., Tapotubun, E.J., Fransina, E.G., Mailoa, M.N., Riry, W.A., Setha, B. and Ri-euwpassa, F., 2021. Seaweed Caulerpa sp position as Functional Food. IOP Conference Series: Earth and Envi-ronmental Science, 517, 012021. https://doi.org/10.1088/1755-1315/517/1/012021
Wilapangga, A. and Syaputra, S., 2018. Ana-lisis Antibakteri Metode Agar Cakram dan Uji Toksisitas Menggunakan BSLT (Brine Shrimp Lethality Test) dari Ekstrak Metanol Daun Salam (Eugenia polyantha). Indonesian Journal of Bio-technology and Biodiversity, 2(2), pp.50-56. https://doi.org/10.47007/ijobb.v2i2.20
Yap, W.F., Tay, V., Tan, S.H., Yow, Y.Y. and Chew, J., 2019. Decoding Antioxidant and Antibacterial Potentials of Malay-sian Green Seaweeds: Caulerpa race-mosa and Caulerpa lentillifera. Antibi-otics, 8(3), 152. https://doi.org/10.3390/antibiotics8030152
Yuan, G., Guan, Y., Yi, H., Lai, S., Sun, Y. and Cao, S., 2021. Antibacterial Activity and Mechanism of Pant Flavonoids to Gram-Positive Bacteria Predicted from Their Lipophilicities. Scientific Reports, 11, 10471. https://doi.org/10.1038/s41598-021-90035-7
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