Effect of Artemisia vulgaris Supplementation on Zebrafish Embryo Under Heat Stress Condition during In Vitro Culture
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Background: Artemisia vulgaris contains flavonoids, which play a vital role in counteracting free radicals. Purpose: To determine the effect of Artemisia vulgaris extract supplementation on embryo development, heart rate and survival of zebrafish under heat stressed and non-heat stressed conditions. Methods: The research used a completely randomized design. Zebrafish embryos (n=240) were divided into heat stressed (36°C) and non-heat stressed (28°C) groups, while for each group were divided into three subgroups, namely T1/control (without Artemisia vulgaris supplementation); and supplemented group T1 and T2, with 2 µL and 4 µL of Artemisia vulgaris supplementation, respectively. The efficacy of Artemisia vulgaris supplementation was determined by observing the embryo development, heart rate, and survival rate of zebrafish up to 96 hours post fertilization (hpf). Results: The development of zebrafish embryos under heat stressed treated with Artemisia vulgaris extract gave the same quality as the control treatment without heat stressed exposure. Zebrafish embryos exposed to heat stressed with 4 µL Artemisia vulgaris supplementation gave the highest survival rate on the heat stressed group. Artemisia vulgaris supplementation improved the heart rate of zebrafish exposed to heat stressed as in the non-heat stressed group. Conclusion: Artemisia vulgaris extract can reduce the detrimental effects of heat stressed induction on zebrafish embryos, as evidenced by the improvement in embryonic development, heart rate, and survival rate of zebrafish embryos after supplementation.
Bensch, R., Song, S., Ronneberger, O., and Driever, W., 2013. Non-Directional Radial Intercalation Dominates Deep Cell Behavior During Zebrafish Epiboly. Biology Open, 2 (8), 845–854.
Dayana, D., 2020. Kajian Pustaka Aktivitas Antioksidan dan Antibakteri Dari Ekstrak Artemisia vulgaris L. [Final Reports]. Universitas Bhakti Kencana.
Febrina, L., Riris, I.D., and Silaban, S., 2017. Uji Aktivitas Antibakteri Terhadap Escherichia coli dan Antioksidan Dari Ekstrak Air Tumbuhan Binara (Artemisia vulgaris L.). Jurnal Pendidikan Kimia, 9 (2), 311–317.
Harper, C. and Lawrence, C., 2011. The Laboratory Zebrafish. Ist Edition. Boca Raton: CRC Press.
Iftikar, F.I. and Hickey, A.J.R., 2013. Do Mitochondria Limit Hot Fish Hearts? Understanding the Role of Mitochondrial Function with Heat Stress in Notolabrus celidotus. PLoS ONE, 8 (5), 1-8.
Kimmel, C.B., Ballard, W.W., Kimmel, S.R., Ullmann, B., and Schilling, T.F., 1995. Stages of embryonic development of the Zebrafish. Developmental Dynamics, 203 (3), 253–310.
Handoyo, D.L.Y., and Pranoto, M.E., 2020. Pengaruh Variasi Suhu Pengeringan Terhadap Pembuatan Simplisia Daun Mimba (Azadirachta Indica). Jurnal Farmasi Tinctura, 1 (2), 45–54.
Lee, S., Ji, K., and Choi, K., 2014. Effects of Water Temperature on Perchlorate Toxicity To The Thyroid and Reproductive System of Oryzias latipes. Ecotoxicology and Environmental Safety, 108, 311–317.
Logan, C.A. and Somero, G.N., 2011. Effects of Thermal Acclimation on Transcriptional Responses To Acute Heat Stress In The Eurythermal Fish Gillichthys Mirabilis (Cooper). American Journal of Physiology: Regulatort, Integrative and Comparative Physiology, 300, 1373–1383.
Luo, S.W., Cai, L., Liu, Y., and Wang, W.N., 2014. Functional Analysis of a Dietary Recombinant Fatty Acid Binding Protein 10 (FABP10) on The Epinephelus coioides in Response to Acute Low Temperature Challenge. Fish and Shellfish Immunology, 36 (2), 475–484.
Mariana, S., Alfons, and Badr, G., 2019. Impact of Heat Stress on The Immune Response of Fishes. Journal of Survey in Fisheries Sciences, 5 (2), 149–159.
Meng, X. liang, Liu, P., Li, J., Gao, B.Q., and Chen, P., 2014. Physiological Responses of Swimming Crab Portunus trituberculatus Under Cold Acclimation: Antioxidant Defense and Heat Shock Proteins. Aquaculture, 434, 11–17.
Ningsih, R.N., 2018. Pengaruh Pemberian Ekstrak Etanol Centella Asiatica Terhadap Motilitas Taktil Dan Panjang Badan Larva Zebrafish (Danio Rerio) Model Hipoksia. [Thesis]. Universitas Brawajaya.
Pörtner, H.O., and Knust, R., 2007. Climate Change Affects Marine Fishes Through The Oxygen Limitation of Thermal Tolerance. Science, 315 (5808), 95–97.
Pörtner, H.O., Mark, F.C., and Bock, C., 2004. Oxygen Limited Thermal Tolerance in Fish? Answers Obtained by Nuclear Magnetic Resonance Techniques. Respiratory Physiology and Neurobiology, 141 (3), 243–260.
Qiu, J., Wang, W.N., Wang, L.J., Liu, Y.F., and Wang, A.L., 2011. Oxidative Stress, DNA Damage and Osmolality in the Pacific White Shrimp, Litopenaeus vannamei Exposed to Acute Low Temperature Stress. Comparative Biochemistry and Physiology-C Toxicology and Pharmacology, 154 (1), 36–41.
Reed, B. and Jennings, M., 2010. Guidance on The Housing and Care of Zebrafish (Danio rerio) Department Science Group RSPCA.
Residiwati G., Azari-Dolatabad N., Tuska, H.S.A., Sidi, S., Van Damme, P., Benedetti, C., Montoro, A.F., Luceno, N.L., Budiono, Pavani, K.C., Opsomer, G., Van Soom, A., and Bogado Pascottini, O., 2021. Effect of Lycopene Supplementation to Bovine Oocytes Exposed to Heat Shock During In Vitro Maturation. Theriogenology, 173, 48-55.
Sampurna, B.P., Audira, G., Juniardi, S., Lai, Y.H., and Hsiao, C.D., 2018. A Simple ImageJ-Based Method to Measure Cardiac Rhythm in Zebrafish Embryos. Inventions, 3 (2), 1-11.
Sholiha, A., 2018. Pengaruh Paparan MSG (Monosodium Glutamate) Dengan Dosis Yang Berbeda Terhadap Perkembangan Embrio Ikan Zebra (Denio rerio). [Thesis]. Universitas Brawijaya.
Spence, R., Gerlach, G., Lawrence, C., and Smith, C., 2008. The behaviour and ecology of the Zebrafish, Danio rerio. Biological Reviews, 83 (1), 13–34.
Thiagarajan, S.K., Rama Krishnan, K., Ei, T., Husna Shafie, N., Arapoc, D.J., and Bahari, H., 2019. Evaluation of the Effect of Aqueous Momordica charantia Linn. Extract on Zebrafish Embryo Model through Acute Toxicity Assay Assessment. Evidence-based Complementary and Alternative Medicine, 2019, 1-9
Wu, C.X., Zhao, F.Y., Zhang, Y., Zhu, Y.J., Ma, M.S., Mao, H.L., and Hu, C.Y., 2012. Overexpression of Hsp90 from Grass Carp (Ctenopharyngodon idella) Increases Thermal Protection Against Heat Stress. Fish and Shellfish Immunology, 33 (1), 42–47.
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