A Comparative Study of Randu Honey Antimicrobial Activity from Several Region in Java
Background: Randu honey is monofloral honey sourced from one type plant nectar. The geographical location of randu (Ceiba pentandra) as the source of nectar is one factor that influences antimicrobial activity produced by randu honey. This research used randu honey from several regions in Java such as Sidoarjo (RSH), Pusat Perlebahan Nasional Bogor (RBH), Kediri (RKH), and Malang (RMH). Objective: To compare the antimicrobial activity of several randu honeys (RSH,RBH,RKH,RMH) against Gram-negative Escherichia coli ATCC 25922, Gram-positive Staphylococcus aureus ATCC 6538 and methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33592, Candida albicans ATCC 10231. Methods: This study used well diffusion and dilution antimicrobial test methods. The diameter of inhibition zone formed in well diffusion method is measured using a vernier caliper. The diffusion method was used as screening test before determining quantitative minimum inhibitory concentration (MIC) using serial dilution with a ratio1:2 (v/v). Streptomycin and Ketoconazole were used as positive controls. Nutrient broth and Sabouraud broth media were incubated at 37°C for 24 hours (antibacterial tests) and 25°C for 48 hours (antifungal test) respectively. Results: Well diffusion test revealed all randu honey samples could inhibit test bacteria and fungi with the appearance of an inhibition zone. Diameter inhibition zone ranged from 14.66±0.52mm to 27.86±0.43mm. The MICs of RSH,RBH,RKH,RMH ranged from 3.12% to 25% against all test bacteria and fungi. Conclusion: The results of this study showed randu honey from Bogor (RBH) has the highest antimicrobial activity based on diffusion and dilution tests.
Abu-Zaid, A.A. Al-Barty, A., Morsy, K., & Hamdi, H. (2022). In Vitro Study of Antimicrobial Activity of Some Plant Seeds Against Bacterial Strains Causing Food Poisoning Diseases. Brazilian Journal of Biology; 82; 1–7. doi: 10.1590/1519-6984.256409.
Akinduti, P.A. Motayo, B., Idowu, O. M., Isibor, P. O., Olasehinde, G. I., Obafemi, Y. D., Ugboko, H. U., Oyewale, J. O., Oluwadun, A., & Adeyemi, G. A. (2019). Suitability of Spectrophotometric Assay for Determination of Honey Microbial Inhibition. Journal of Physics: Conference Series; 1299(1); 1-8. doi: 10.1088/1742-6596/1299/1/012131.
Almasaudi, S. (2021) The Antibacterial Activities of Honey. Saudi Journal of Biological Sciences; 28(4); 2188–2196. doi: 10.1016/j.sjbs.2020.10.017.
Anand, S., Deighton, M., Livanos, G., Pang, E.C.K., & Mantri, N. (2019). Agastache Honey has Superior Antifungal Activity in Comparison with Important Commercial Honeys. Scientific Reports; 9(1); 1–14. doi: 10.1038/s41598-019-54679-w.
Badan Pusat Statistik (2018). Statistik Produksi Kehutanan, Penelitian Terapan Kajian Strategi Nasional. Jakarta: Badan Pusat Statistik.
Banerjee,N., Biswas,S., Hossain,C.M., & Basak, P.(2022) Chapter 12 - Effectiveness of Onion (Allium cepa L.) Skin in Human Health In: Contemporary Medical Biotechnology Research for Human Health; 115-125. England: Elsevier Inc.
Basuki, T. (2018). Bunga Kapuk (Ceiba pentandra L. Gaertn) sebagai Sumber Pakan Lebah Madu yang Potensial, Balittas; 5; 345–351.
Brudzynski, K. (2020). A Current Perspective on Hydrogen Peroxide Production in Honey: A Review. Food Chemistry; 332;127-229. doi: 10.1016/j.foodchem.2020.127229.
Burgut, A. (2020). Volatile Aromatic Composition and Antimicrobial Activity of Different Types of Honey. Progress in Nutrition; 22(3); 1-10. doi: 10.23751/pn.v22i3.8495.
Clearwater, M.J., Revell, M., Noe, S., & Manley-Harris, M. (2018). Influence of Genotype, Floral Stage, and Water Stress on Floral Nectar Yield and Composition of Mānuka (Leptospermum scoparium); Annals of Botany; 121(3); 501–512. doi: 10.1093/AOB/MCX183.
Dewi, M.A., Kartasasmita, R.E. & Wibowo, M.S. (2017). Uji Aktivitas Antibakteri Beberapa Madu Asli Lebah asal Indonesia terhadap Staphylococcus aureus dan Escherichia coli. Jurnal Ilmiah Farmasi; 5(1); 27–30.
Dezmirean, D. S., Mărghitaş, L. A., Chirilă, F., Copaciu, F., Simonca, V., Bobiş, O., & Erler, S. (2017). Influence of Geographic Origin, Plant Source and Polyphenolic Substances on Antimicrobial Properties of Propolis Against Human and Honey Bee Pathogens. Journal of Apicultural Research; 56(5); 588–597. doi: 10.1080/00218839.2017.1356205.
Djakaria, S.A., Batubara, I. & Raffiudin, R. (2020). Antioxidant and Antibacterial Activity of Selected Indonesian Honey Against Bacteria of Acne. Jurnal Kimia Sains dan Aplikasi; 23(8); 267–275. doi: 10.14710/jksa.23.8.267-275.
Hasan, A.E.Z., Herawati, H., Purnomo, P., & Lathifah, A. (2020) ‘Fisikokimia Madu Multiflora asal Riau dan Potensinya sebagai Antibakter Escherichia coli dan Staphylococcus aureus’, Chemistry Progress, 13(2), pp. 81–90. doi: 10.35799/cp.13.2.2020.31594.
Irfanah, L. (2018). Perbandingan Metode Difusi Agar dan Dilusi secara Turbidimetri Untuk Uji Daya Hambat Madu Randu terhadap Pertumbuhan Bacillus subtilis ATCC 6633. Skripsi: Fakultas Farmasi Universitas Airlangga, Surabaya.
Irish, J., Blair, S. & Carter, D.A. (2021) The Antibacterial Activity of Honey Derived from Australian Flora. PLoS ONE; 6(3); 1-9. doi: 10.1371/journal.pone.0018229.
Kemenkes RI. (2020). Farmakope Indonesia Edisi VI. Jakarta: Kementerian Kesehatan RI.
Kocsis, M. Bodó, A., Kőszegi, T., Csepregi, R., Filep, R., Hoffmann, G., & Farkas, Á. (2022). Quality Assessment of Goldenrod, Milkweed and Multifloral Honeys Based on Botanical Origin, Antioxidant Capacity and Mineral Content. International Journal of Molecular Sciences; 23(2); 1-13. doi: 10.3390/ijms23020769.
Kuete V. (2010) Potential of Cameroonian Plants and Derived Products Against Microbial Infections: A Review. Planta Med; 76; 1479-1491.
Tomczyk, M., Tarapatskyy, M. & Dżugan, M. (2019). The Influence of Geographical Origin on Honey Composition Studied by Polish and Slovak Honeys. Czech Journal of Food Sciences; 37(4); 232–238. doi: 10.17221/40/2019-CJFS.
Vaou, N., Elisavet, V., Chrysa, T., & Bezirtzoglou, E. (2021). Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives. Microorganisms; 9; 1–28. doi: 10.3390/microorganisms9102041.
Widodo, S., Khasanah, R., Parman, S., & Suedy, A. (2017). Kualitas Madu Lokal dari Lima Wilayah di Kabupaten Kediri. Jurnal Biologi; 6 (1); 29–37.
Copyright (c) 2024 JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1. The copyright of this journal belongs to the Editorial Board and Journal Manager with the author's knowledge, while the moral right of the publication belong to the author.
2. The formal legal aspect of journal publication accessibility refers to the Creative Commons Attribution-Non-Commercial-Share Alike (CC BY-NC-SA), which implies that the publication can be used for non-commercial purposes in its original form.
3. Every publication (print/electronic) is open access for educational, research, and library purposes. In addition to the objectives mentioned above, the editorial board is not responsible for copyright infringement
.jpg)
