Toksisitas Larutan Perak Nitrat (AgNO3) pada Mencit Balb-c Berdasarkan Kadar SGPT dan SGOT

Indah Amalia Amri, Muhamad Ferian Hendrasmara, Dahliatul Qosimah, Ajeng Aeka, Nofan Rickyawan, Wawid Purwatiningsih, Fidi Nur Aini Eka Puji Dameanti

= http://dx.doi.org/10.20473/jmv.vol3.iss2.2020.251-257
Abstract views = 421 times | downloads = 1684 times

Abstract


Perak Nitrat (AgNO3) sering dimanfaatkan di bidang kesehatan sebagai bahan antimikroba. Efek pada organisme yang terpapar perak nitrat dapat mengakibatkan adanya akumulasi toksik pada hepar. Penelitian ini bertujuan mengetahui dampak toksisitas perak nitrat terhadap kadar SGPT dan SGOT pada mencit Balb-c. Hewan coba yang digunakan pada penelitian ini berjumlah 20 ekor mencit Balb-c dengan berat badan 25-30  gram dan umur 2-3 bulan serta masa pemberian perlakuan selama 14 hari. Perlakuan yang diberikan terdiri dari kelompok K- (tanpa induksi), P1 (100 µg/ml), P2 (200 µg/ml), P3 (300 µg/ml), P4 (400 µg/ml). Kadar SGPT dan SGOT diamati dengan menggunakan metode spektrofotometri. Data dianalisa menggunakan uji One Way ANOVA dengan tingkat kepercayaan 95%. Hasil dan kesimpulan menunjukkan induksi larutan perak nitrat pada mencit Balb-c dengan dosis P4 (400 µg/ml) dan volume induksi 0.5 ml dapat menyebabkan toksisitas pada mencit yang ditandai dengan peningkatan kadar SGPT dan SGOT.

Keywords


larutan perak nitrat; mencit; SGPT; SGOT

Full Text:

PDF

References


Almonte, T.J., Barnes, J.H., Bautista, S.C., De La Cruz, B., Lili, L., Paciencia, A.P. 2009. Serum Transaminase in mice after Whole-body X-irradiation and the Effect of a Chemical Protector. Int. J. Rad. Biol. Related Study in Phys. Chem. Med., 9(1), 37-42.

Banerjee, P., Nath, D. 2015. A phytochemical approach to synthesize silver nanoparticles for non-toxic biomedical application and study on their antibacterial efficacy. Nanosci. Technol., 2(1), 1-14.

Bolliger, A.P., Nancy, E. 2012. Haematology of the Mouse. In Hendrich, H. J. (Ed). The Laboratory Mouse. London: Elseiver, pp: 331-345.

Chauhan, P.S., Shrivastava, V.I.K.A.S., Prasad, G.B.K.S., Tomar, R.S., Shrivastava, V. 2018. Effect of Silver Nanoparticle-Mediated Wound Therapy on Biochemical, Hematological, and Histological Parameters. APCR, 11(3), 251-258.

Chiang, J. 2014. Liver Physiology: Metabolism and Detoxification. In: Linda M. M., Richard N. M. Pathobiology of Human Disease. San Diego: Elsevier, pp: 1770-1782.

Curf, J.H.A.J., André, C., Bart, S.S., Merel, R.H. 2010. Handbook of Laboratory Animal Science: Nutrient Requirements, Experimental Design, and Feeding Scedules in Animal Experimentation. In Hau, J. and Steven J. S. Handbook of Laboratory Animal Science. Boca Raton: CRC Press, pp: 306-340.

Elmorsy, E., Mohamed M.S., Mostafa, A., Lucky, L.N., Maysaa, S.Z., Mahmoud, B., Nahla, A., Amal, M.A. 2015. Liver Functions Deragement Among Subtances Abusers. Sci. J. Impact Factor, 6(3), 19-26.

Govind, P., Madshuri, S. 2014. Heavy Metals Causing Toxicity in Animal and Fishes. Res. J. Anim. Vet. Fish. Sci., 2(2), 17-23.

Kendran, A.A.S., Anak, A.G.A., Anak, A.S.I.P. 2017. Aktivitas Enzim Alanine-Aminotransferase dan Aspartat Aminotransferase pada Tikus Putih Jantan yang Diberi Ekstrak Buah Pinang. Buletin Veteriner Udayana, 9(2), 132-138.

Kurniawati, I., Titis, N., Taufik, N.Y. 2015. Effect of Giving Etanol Multistep Does to Level of SGPT and SGOT in Wistar Rats (Rattus novergicus). Indon. J. Med. Health, 7(1), 30-35.

Mahdi, C., Chandra, A.P., Herlina, P. 2019. Preventive Study Garlic Extract Water (Allium sativum) Toward SGPT, SGOT, and the Description of Liver Histopatology on Rat (Rattus novergicus), which were exposed by Rhodamine B. Malang, Indonesia, 20-21 March 2019, pp: 062015.

Pandian, S.R.K., Venkataraman, D., Kalimuthu, K., Pushpa, V., Sangiliyandi, G. 2010. Mechanism of Bacterial Activity of Silver Nitrate-A Concentration Dependent Bifunctional Molecule. Brazil. J. Microbiol., 41(3), 805-809.

Prasannaraj, G., Venkatachalam, P. 2017. Hepatoprotective effect of engineered silver nanoparticles coated bioactive compounds against diethylnitrosamine induced hepatocarcinogenesis in experimental mice. J. Photochem. Photobiol. B: Biol., 167, 309-320.

Ristian, I. 2013. Kajian Pengaruh Konsentrasi Perak Nitrat (AgNO3) Terhadap Ukuran Nanopartikel Perak [Skripsi]. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Negeri Semarang. Hal: 1.

Rosida, A. 2016. Pemeriksaan Laboratorium Penyakit Hati. Berkala Kedokteran, 12(1), 123-131.

Sekarwati, N., Bardi, M., Sunarto. 2015. Dampak Logam Berat Cu (Tembaga dan Ag (Perak) pada Limbah Cair Industri Perak Terhadap Kualitas Air Sumur dan Kesehatan Masyarakat serta Upaya Pengendaliannya di Kota Gede Yogyakarta. Jurnal Eko Sains, 1(1), 663-671.

Shofi, M. 2017. Daya Hambat Perak Nitrat (AgNO3) pada Perkecambahan Biji Kacang Hijau (Vigna radiata). Al Kauniyah: J. Biol., 10(2), 98-104.

Subramaniyan, S.A., Belal, S.A., Choe, H.S., Shim, K.S. 2018. A Comparative Study of Biologically and Chemically Fabricated Synthesized AgNPs’ Supplementation with Respect to Heat-Shock Proteins, Survival, and Hatching Rates of Chicken Embryos: An in ovo Study. J. Clust. Sci., 29(1), 129-139.

Technical Resources. 2012. BALB/C Mouse Hematology. Charles River Laboratories. United of State. pp: 1-2.

Teran, C.G., Sunitha, S., Peminda, C., Casey, B. 2011. Silver Nitrate Ingestion: Report of A Case with an Uneventful Course and Review. Clin. Pract., 1(43), 82-83.

Widayati, E. 2012. Oksidasi Biologi, Radikal Bebas, dan Antioxidant. Jurnal Majalah Ilmiah Sultan Agung, 50(128), 26-32.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Indah Amalia Amri

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Medik Veteriner (JMV) Indexed by:

                            

Editorial Office

Jurnal Medik Veteriner
Kampus PSDKU Banyuwangi Universitas Airlangga
Jalan Wijaya Kusuma No.113, Giri, Banyuwangi, 68425 
Email: jmv@psdku.unair.ac.id
Telp: 0333-417788

pISSN 2615-7497 ; eISSN 2581-012X

View My Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.