Risk Factors and Incidence of Escherichia coli Producing Extended-spectrum β-lactamase (ESBL) in Dairy Cattle
This study aimed to evaluate the risk factor and insidence of Extended-spectrum b-lactamase (ESBL) produced by Escherichia coli in dairy cattle. The samples obtained were 38 rectal swabs from dairy cows from the Successful Mutual Livestock Group, Deyeng Village, Kediri Regency, East Java. The study started with interviews and observations using a questionnaire to obtain data on risk factors (source of drinking water, cleanliness of feedlots, cleanliness of drinking places, administration of antibiotics, history of mastitis). A sampling of the rectal swab of cattle was carried out using Nutrient Broth as carrier media. Isolation of bacteria using Mac Conkey Agar (MCA) media with the antibiotic cefotaxime 1 mg/L. The addition of antibiotics is intended so that the bacteria that grow are coliform bacteria resistant to the cefotaxime antibiotic, a beta-lactam group. Bacterial isolation was continued with MCA media to enrich ESBL bacteria. Bacterial identification was continued on EMBA media, gram staining, IMViC biochemical test, TSIA, and urease to obtain isolates of E. coli bacteria. The E. coli isolates tested for ESBL Double Disc Synergy Test (DDST) phenotype. The close relationship between each risk factor and the incidence of ESBL-producing E. coli was tested statistically using the Spearman Rank correlation test. The results showed that the incidence of ESBL-producing E. coli in the tested samples was 21.05%, or there were eight positive samples of ESBL-producing E. coli. The risk factors for finding a positive incidence of E. coli producing ESBL in this study were 87% of the use of drinking water sources from wells, 25% did not pay attention to the cleanliness of drinking places, 25% did not pay attention to the cleanliness of eating places, 38% were given antibiotics during maintenance, and 100% had a history of mastitis. These risk factors were not statistically closely related to the incidence of ESBL-producing E. coli.
Batabyal, K., Banerjee, A., Pal, S., Dey, S., Joardar, S. N., Samanta, I., Isore, D. P., & Singh, A. D. (2018). Detection, characterization, and antibiogram of extended-spectrum beta-lactamase Escherichia coli isolated from bovine milk samples in West Bengal, India. Veterinary World, 11(10), 1423–1247.
Dahmen, S., Métayer, V., Gay, E., Madec, J.-Y., & Haenni, M. (2013). Characterization of extended-spectrum beta-lactamase (ESBL)-carrying plasmids and clones of Enterobacteriaceae causing cattle mastitis in France. Veterinary Microbiology, 162(2–4), 793–799.
Gay, N., Leclaire, A., Laval, M., Miltgen, G., Jégo, M., Stéphane, R., Jaubert, J., Belmonte, O., & Cardinale, E. (2018). Risk Factors of Extended-Spectrum β-Lactamase Producing Enterobacteriaceae Occurrence in Farms in Reunion, Madagascar and Mayotte Islands, 2016–2017. Veterinary Sciences, 5(1), 22.
Guenther, S., Ewers, C., & Wieler, L. H. (2011). Extended-Spectrum Beta-Lactamases Producing E. coli in Wildlife, yet Another Form of Environmental Pollution? Frontiers in Microbiology, 2.
Gyles, C., & Boerlin, P. (2014). Horizontally Transferred Genetic Elements and Their Role in Pathogenesis of Bacterial Disease. Veterinary Pathology, 51(2), 328–340.
Halasa, T., Huijps, K., Østerås, O., & Hogeveen, H. (2007). Economic effects of bovine mastitis and mastitis management: A review. Veterinary Quarterly, 29(1), 18–31.
Kamaruzzaman, E. A., Abdul Aziz, S., Bitrus, A. A., Zakaria, Z., & Hassan, L. (2020). Occurrence and Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Dairy Cattle, Milk, and Farm Environments in Peninsular Malaysia. Pathogens, 9(12), 1007.
Livermore, D. M., & Brown, D. F. J. (2001). Detection of β-lactamase-mediated resistance. Journal of Antimicrobial Chemotherapy, 48(suppl_1), 59–64.
Lu, S.-Y., Zhang, Y.-L., Geng, S.-N., Li, T.-Y., Ye, Z.-M., Zhang, D.-S., Zou, F., & Zhou, H.-W. (2010). High Diversity of Extended-Spectrum Beta-Lactamase-Producing Bacteria in an Urban River Sediment Habitat. Applied and Environmental Microbiology, 76(17), 5972–5976.
Maulana, Y. K., Duangporn, P., Nur, R. H., Dyah, A. W., Fred, U., Veerasak, P., & Tongkorn, M. 2021. Antimicrobial Resistance Characteristics of Extended Spectrum Beta Lactamase (ESBL)- Producing Escherichia coli from Dairy Farms in the Sleman District of Yogyakarta Province, Indonesia. Veterinary Integrative Sciences, 19(3): 525-535.
Oliver, S. P., Jayarao, B. M., & Almeida, R. A. (2005). Foodborne Pathogens in Milk and the Dairy Farm Environment: Food Safety and Public Health Implications. Foodborne Pathogens and Disease, 2(2), 115–129.
Pratama, A. S., Djide, M. N., & Massi, M. N. (2019). Identifikasi Genotip CTX-M PADA Escherichia coli Penghasil Extended Spectrum Beta Lactamase (ESBL) yang Resisten pada Cephalosporin Generasi III di RSUP Wahidin Sudirohusodo Makassar. Majalah Farmasi dan Farmakologi, 23(1), 5–9.
Rao, L., Lv, L., Zeng, Z., Chen, S., He, D., Chen, X., Wu, C., Wang, Y., Yang, T., Wu, P., Liu, Y., & Liu, J.-H. (2014). Increasing prevalence of extended-spectrum cephalosporin-resistant Escherichia coli in food animals and the diversity of CTX-M genotypes during 2003–2012. Veterinary Microbiology, 172(3–4), 534–541.
Schmid, A., Hörmansdorfer, S., Messelhäusser, U., Käsbohrer, A., Sauter-Louis, C., & Mansfeld, R. (2013). Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli on Bavarian Dairy and Beef Cattle Farms. Applied and Environmental Microbiology, 79(9), 3027–3032.
Seni, J., Falgenhauer, L., Simeo, N., Mirambo, M. M., Imirzalioglu, C., Matee, M., Rweyemamu, M., Chakraborty, T., & Mshana, S. E. (2016). Multiple ESBL-Producing Escherichia coli Sequence Types Carrying Quinolone and Aminoglycoside Resistance Genes Circulating in Companion and Domestic Farm Animals in Mwanza, Tanzania, Harbor Commonly Occurring Plasmids. Frontiers in Microbiology, 7.
Suryani, S., Roza, R. M., dan Martina, A. (2014). Seleksi dan Uji Antibakteri Aktinomisetes Asal Tanah Gambut Rimbo Panjang Kampar Riau terhadap Escherichia coli dan Salmonella typhi. JOM FMIPA, 1(2), 11.
Timofte, D., Maciuca, I. E., Evans, N. J., Williams, H., Wattret, A., Fick, J. C., & Williams, N. J. (2014). Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-Lactamases from Bovine Mastitis Isolates in the United Kingdom. Antimicrobial Agents and Chemotherapy, 58(2), 789–794.
Weber, L. P., Dreyer, S., Heppelmann, M., Schaufler, K., Homeier-Bachmann, T., & Bachmann, L. (2021). Prevalence and Risk Factors for ESBL/AmpC-E. coli in Pre-Weaned Dairy Calves on Dairy Farms in Germany. Microorganisms, 9(10), 2135.
Xu, G., An, W., Wang, H., & Zhang, X. (2015). Prevalence and characteristics of extended-spectrum β-lactamase genes in Escherichia coli isolated from piglets with post-weaning diarrhea in Heilongjiang province, China. Frontiers in Microbiology, 6.
Copyright (c) 2022 Fidi Nur Aini Eka Puji Dameanti, Muhammad Ali Akramsyah, Chyntia Silvi Yanti Hasan, Jacky Teguh Amanda, Alfaro Rikko Pratama, Reza Fahmiantika, Dhaneswara Tedja, Safira Izofani, Rahayu Sutrisno
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish in this journal agree to the following terms:
1. The journal allows the author to hold the copyright of the article without restrictions;
2. The journal allows the author(s) to retain publishing rights without restrictions;
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA).