Histopathological and Gastric Histomorphometry Analysis in Mice Colibacillosis Model Induced by Ethanol 96% Oregano Vulgare Extract
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Colibacillosis is a disease caused by Escherichia coli which is a normal flora bacterium of the digestive system in humans and animals. Oregano contains carvacrol and thymol which provide an antibacterial. This study was used 20 male Balb/C mice age 2-3 weeks with an average weight of 20-25 grams and were divided into 5 groups with 4 replications: negative control (KN), positive control (KP) given by streptomycin dose of 20 mg/head. E. coli dose of 3 x 109 CFU, (P1) was given by streptomycin dose of 20 mg/head, E. coli dose of 3 x 109 CFU, then given oreganoextract dose of 5 mg/head, (P2) was given by streptomycin dose of 20 mg/head, E. coli dose of 3 x 109 CFU, then given oregano extract dose of 10 mg/head, (P3) was given by streptomycin dose of 20 mg/head, E. coli dose of 3 x 109 CFU, then oregano extract was given dose of 20 mg/head. The data were analyzed by descriptive comparative. The histopathology showed epithelial cell damage or erosion caused by inflammatory cells in KP, P1, P2, and P3. In study group given oregano extract dose of 20 mg/head, P3, inflammatory cells and epithelial erosion were lower. Based on the ANOVA test of day 3 and 6 after necropsy, P1, P2, and P3 show insignificant in villi length and width of the vili (p>0,05) compared to KP. The conclusion the result of the histopathology result is epithelial erosion in group with dose of 20mg/head oregano extract was able to relieve the symptoms of colibacillosis. The conclusion of histomorphometry of the length and width of the villi at P1, P2, P3 (p>0.05) was not significantly different from the positive control.
Alekseeva, M., Tzvetelina, Z., Ivan, A., & Krasimir, R. (2020). Origanum Vulgare L. – A Review on Genetic Diversity, Cultivation, Biological Activities and Perspectives for Molecular Breeding. Bulgarian Journal of Agricultural Science, 26(06).
Ananda, R. F. (2020). Efek Gastroprotektif Ekstrak Etanol Daun Bangun-Bangun (Plectranthus amboinicus (lour.) Spreng.) Pada Lambung Tikus Putih Jantan (Rattus norvegicus) Yang diinduksi Dengan Metode Stres. Fakultas Farmasi. Universitas Sumatera Utara.
Aspinall, V., & Cappello, M. (2015). Introduction to Veterinary Anatomy and Physiology Textbook. Third Edition. Edinburgh ; New York: Elsevier.
Bukovska, A., Cikos, S., & Juhas, S. (2007). Effects of a Combination of Thyme and Oregano Essential Oils on Tnbs-Lnduced Colitis in Mice. Mediators of Inflammation. pp: 10.
Chevallier, A. (2016). Encyclopedia of Herbal Medicine. Third. American: Dorling Kindersley.
Donnenberg, M. S. (2013). Escherichia Coli: Pathotypes and Principles of Pathogenesis. 2nd Edition. Amsterdam: Academic Press.
Fajeria, A. L. (2020). Pengaruh Preventif Ekstrak Daun Dewandaru (Eugenia Uniflora. L) Terhadap Kadar Malondialdehyde (MDA) Dan Histopatologi Duodenum Pada Tikus Putih (Rattus Norvegicus) Model Gastroenteritis Induksi Escherichia Coli. Media Kedokteran Hewan, 31(2), 74.
Kaiser, P., Diard, M., Stecher, B., & Hardt, W. D. (2012). The Streptomycin Mouse Model For Salmonella Diarrhea: Functional Analysis Of The Microbiota, The Pathogen's Virulence Factors, And The Host's Mucosal Immune Response: Streptomycin Mouse Model', Immunological Reviews, 245(1), 56–83.
Kartika, A. A., Siregar, H. C. H., & Fuah, A. M. (2013). Strategi Pengembangan Usaha Ternak Tikus (Rattus Norvegicus) Dan Mencit (Mus Musculus) di Fakultas Peternakan IPB, Jurnal Ilmu Produksi Dan Teknologi Hasil Peternakan, 01(3).
Miladiyah, I. 2010.Toksidinamika Antibiotikka Golongan Aminoglikosida', Jkki, 2(5).
Muhtadi, S., Andi, W., Nurcahyanti, & Sutrisna. (2014). Uji Praklinik Antihiperurisemia Secara In Vivo Pada Mencit Putih Jantan Galur Balb-C Dari Ekstrak Daun Salam (Syzigium Polyanthum Walp) Dan Daun Belimbing Wuluh (Averrhoa Bilimbi L.)', Biomedika, 6(1).
Muliani, H. (2011). Pertumbuhan Mencit (Mus Musculus L.) Setelah Pemberian Biji Jarak Pagar (Jatropha Curcas L.)', 1, 11.
Nugroho, R. (2018). Mengenal Mencit Sebagai Hewan Laboratorium. Samarinda: Mulawarman University Press.
París, J. R., Ana, R., & Beltran, N. (2016). Morphological Mouse Phenotyping: Anatomy, Histology and Imaging. Paris: Elsevier.
Prasetya, Y. A., Yuyun, W. I., Aprilia, P. K., Chorry, H. M., & Nur, R. D. (2019). Deteksi Fenotipik Escherichia Coli Penghasil Extended Spectrum Beta-Lactamases (Esbls) Pada Sampel Makanan Di Krian Sidoarjo', Life Science, 8(1).
Rejeki, P. S., Cahyaning, P. E. A., & Eka, P. R. (2018). Ovariektomi Pada Tikus Dan Mencit. Airlangga University Press.
Riedel, S., Jeffery, H., Steve, M., Stephen, M., & Timothy, M. (2019). Jawetz Melnick & Adelbergs Medical Microbiology, Twenty-Eighth Edition. 28th Edn. United States: Mc. Grawhill.
Shalihat, H. K. (2018). Potensi Carvacrol Dalam Daun Bangun-Bangun Sebagai Antimikroba dan Imunostimulator, Cermin Dunia Kedokteran, 45(11).
Tarmudji. (2003). Kolibasilosis Pada Ayam: Etiologi, Patologi Dan Pengendaliannya. Wartazoa, 13(02).
Wiralaga, I. P. A., Wayan, S. I., & Made, K. I. (2015). Pengaruh Pemberian Ekstrak Etanol Daun Ashitaba (Angelica Keiskei) Terhadap Histopatologi Lambung Mencit (Mus Musculus) Jantan, 7(1), 7.
Zumrotul, M. (2015). Buah Mengkudu (Morinda citrifolia) Meningkatkan Respon Imun Mencit (Mus Musculus) Terhadap Infeksi Bakteri Staphylococcus Aureus, Journal of Health Sciences, 6(2), 34.
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