The Effect of Apis dorsata Honey Treatment on Calcium Levels of Mandibular Bone in Ovariohysterectomized Wistar Rats
Downloads
This study aimed to evaluate the efficacy of Apis dorsata honey on calcium levels in the mandibular bone of Wistar rats after ovariohysterectomy as an animal osteoporosis model for treating cases of osteoporosis. This study used 20 female rats, which were divided into five treatment groups with four replications. SHAM was the negative control group; OH was the ovariohysterectomized group without treatment as a positive control; the AD1, AD2, and AD3 groups were ovariohysterectomized rats with A. dorsata honey at doses of 1 g/kg bw, 2 g/kg bw, and 4 g/kg bw for 84 days. After 84 days of treatment, the left mandibular bones of the rats were collected, weighed, and stored in 10% neutral buffered formalin. Calcium levels were calculated using proximate analysis. The results showed a decrease in bone calcium levels in the ovariohysterectomy group, the highest results were in the SHAM group, and the lowest results were in the AD3 group. It can be concluded that the administration of A. dorsata honey could not maintain the calcium level of the mandibular bone in the ovariohysterectomized Wistar rats.
Al-Arif, M. A., Nurhajati, T., Sidik, R., Lamid, M., Setyono, H., & Lokapirnasari, W. P. (2016). Teaching Book of Animal Feed Technology. Surabaya: PT Revka Petra Media.
Al-Zuhroh, T. Y., Santoso, K. P., Yunita, M. N., Hidajati, N., & Praja, R. N. (2021). Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea Canephora). Jurnal Medik Veteriner, 4(2), 213–220.
Blair, H. C., Larrouture, Q. C., Li, Y., Lin, H., Beer-Stoltz, D., Liu, L., Tuan. R. S., Robinson, L. J., Schlesinger, P. H., & Nelson, D. J. (2017). Osteoblast Differentiation and Bone Matrix Formation In Vivo and In Vitro. Tissue Engineering Part B: Reviews, 23(3), 268–280.
Boskey, A. L. (2013). Bone Composition: Relationship to Bone Fragility and Antiosteoporotic Drug Effects. BoneKEy reports, 2,447.
Chan, C. K. Y., Mason, A., Cooper, C., & Dennison, E. (2016). Novel Advances in The Treatment of Osteoporosis. British Medical Bulletin, 119(1), 129–142.
Cianciosi, D., Forbes-Hernández, T. Y., Afrin, S., Gasparrini, M., Reboredo-Rodriguez, P., Manna, P. P., Zhang, J., Lamas, L. B., Florez, S. M., Toyos, P. A., Quiles, J. L., Giampieri, F., & Battino, M. (2018). Phenolic Compounds in Honey and Their Associated Health Benefits: A Review. Molecules, 23(9), 2322.
Curtis, E. M., Moon, R. J., Dennison, E. M., Harvey, N. C., & Cooper, C. (2015). Recent Advances in The Pathogenesis and Treatment of Osteoporosis. Clinical Medicine, 15(6), 92–96.
da Silva, P. M., Gauche, C., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2016). Honey: Chemical Composition, Stability and Authenticity. Food Chemistry, 196, 309–323.
Erlangga, M. B., Srianto, P., Safitri, E., Hernawati, T., & Hermadi, H. A. (2023). The Potential of Kaliandra Honey (Calliandra sp.) on Sertoli Cell Counts in Malnourished Albino Rats. Jurnal Medik Veteriner, 6(1), 88–92.
Fikri, F., Purnomo, A., Chhetri, S., & Purnama, M. T. E. (2023). Sea Cucumber-Based Hydroxyapatite-Chitosan Ameliorate Serum Liver Enzymes and Cytokine Levels in Albino Rats with Femoral Bone Defect. Indian Veterinary Journal, 100(7), 23–26.
Gupta, A., & March, L. (2016). Treating Osteoporosis. Australian Prescriber, 39(2), 40–46.
Hwang, Y. H., Kim, K. J., Kim, J. J., Kang, K. Y., Lee, S. J., Jeong, G. Y., Choi, K. H., Son, Y. J., & Yee, S. T. (2015). Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract of Hovenia dulcis) on The Ovariectomized Mice. Evidence-Based Complementary and Alternative Medicine.
Komori, T. (2015). Animal Models for Osteoporosis. European Journal of Pharmacology, 759, 287–294.
Laswati, H., Hendarto, H., Irawati, D., & Mahaputra, L. (2015). Tomato Juice Increase Bone Density of Menopause Albino Rats. Jurnal Veteriner, 16(3), 457–462.
Listyorini, L., Mustofa, I., Hernawati, T., Rimayanti, R., & Suprayogi, T. W. (2021). Honey Can Increase the Length of the Small Intestinal Villi in Malnourished Albino Rats. Jurnal Medik Veteriner, 4(2), 175–179.
Lukmandaru, G., & Hidayah, R. N. (2017). Quality Study of Teak Wood in Gunungkidul People's Forests. VI. Inorganic Substances and Acidity. Jurnal Ilmu Kehutanan, 11(1), 63–75.
Muhamad, R. (2018). Comparison of the Iinfluence of Honey Bees (Apis dorsata) and Honey Bees (Apis mellifera) Based on the Opacity of the Radiography and the Femoral Bone Weight of Albino Rats (Rattus norvegicus) Ovariohysterectomy. Undergraduate Thesis, Faculty of Veterinary Medicine Universitas Airlangga.
Muslim, T. (2014). The potential of forest honey and its management in Indonesia. Prosiding Seminar BALITEK KSDA, pp: 67–82.
Perricone, N., & Madison, C. (2017). Patent No. US 2017/0080016. A1. United States.
Prastiya, R. A., Samik, A., Purnama, M. T. E., & Saputro, A. L. (2019). Protective effect of mycotoxin binders on ovarian gestation mice exposed by zearalenone. Indian Veterinary Journal, 96(9), 44–46.
Safira, A., Rani, C. A. M., Fikri, F., Purnomo, A., Khairani, S., Chhetri, S., Maslamama, S. T., & Purnama, M. T. E. (2023). Hydroxyapatite-chitosan composites derived from sea cucumbers and shrimp shells ameliorate femoral bone defects in an albino rat model. Veterinary World, 16(5), 1084–1091.
Safitri, E., Purnobasuki, H., Purnama, M. T. E., & Chhetri, S. (2022). Effectiveness of forest honey (Apis dorsata) as therapy for ovarian failure causing malnutrition. F1000Research, 11, 512.
Sardjana, I. K., & Kusumawati, D. (2011). Teaching Book of Veterinary Surgery. Airlangga University Press.
Wardati, F. S. (2018). Potential of Honey Bees Apis dorsata and Honey Bees Apis mellifera as Anti-osteoporosis Towards the Amount of Osteoblasts and Osteoclasts in Albino Rats (Rattus norvegicus) Ovariohysterectomy Model. Undergraduate Thesis, Faculty of Veterinary Medicine Universitas Airlangga.
Weliyani, T., Hamid, I. S., Hidanah, S., Prastiya, R. A., & Wibawati, P. A. (2021). Effect of Robusta Coffee Extract on Histopathological in Mice Testes Induced with Monosodium Glutamat. Jurnal Medik Veteriner, 4(2), 243–248.
Yordan, S., Hasib, A., Ibrahim, M. H. R., Rohmah, S. N., Abani, S., & Yudaniayanti, I. S. (2018). Analysis of Scanning Electron Microscope (SEM) Microarchitecture of the Femoral Bone Metaphysical Area in Albino Rats (Rattus novergicus) Ovariohysterectomy Given Honey Bees (Apis dorsata) Sumbawa Forest. Jurnal Sain Veteriner, 36(1), 58–65.
Yudaniayanti, I. S., Lukiswanto, B. S., & Arifin, M. Z. (2017). IL-1β Expression in the Process to Cure Fractures of the Albino Rats (Rattus norvegicus) Ovariectomy Treated with Cikal Tulang Extract (Cissus Quadrangularis). Jurnal Sain Veteriner, 35(1), 91–101.
Yudaniayanti, I. S., Primarizky, H., & Nangoi, L. (2018). The Effects of Honey (Apis dorsata) Supplements on Increased Bone Strength in Ovariectomized Rat as Animal Model of Osteoporosis. AIP Conference Proceedings.
Yudaniayanti, I. S., Primarizky, H., Nangoi, L., & Yuliani, G. A. (2019). Protective Effects of Honey by Bees (Apis dorsata) on Decreased Cortical Thickness and Bone Impact Strength of Ovariohysterectomized Rats as Models for Menopause. Veterinary World, 12(6), 868–876.
Zaid, S. S. M., Sulaiman, S. A., Othman, N. H., Soelaiman, I. N., Shuid, A. N., Mohamad, N., & Muhamad, N. (2012). Protective Effects of Tualang Honey on Bone Structure in Experimental Postmenopausal Rats. Clinics, 67(7), 779–784.
Zaid, S. S. M., Sulaiman, S. A., Sirajudeen, K. N. M., & Othman, N. H. (2010). The Effects of Tualang Honey on Female Reproductive Organs, Tibia Bone and Hormonal Profile in Ovariectomised Rats--Animal Model for Menopause. BMC complementary and Alternative Medicine, 10, 82.
Copyright (c) 2023 Siti Nur Rohmah, Ira Sari Yudaniayanti, Hardany Primarizky, Poedji Hastutiek
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors who publish in this journal agree to the following terms:
1. The journal allows the author to hold the copyright of the article without restrictions;
2. The journal allows the author(s) to retain publishing rights without restrictions;
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA).
11.jpg)
