The differences of effectiveness of β-1,3-glukanase Vigna unguiculata and papain Carica papaya enzymes in hydrolysis of denture plaque

Retno Indrawati, Muhammad Luthfi, Erina Fatmala Yuli Andari

= http://dx.doi.org/10.20473/j.djmkg.v49.i2.p81-86
Abstract views = 577 times | downloads = 705 times

Abstract


Background: Accumulation of denture plaque can lead to pathological changes in oral mucosa, such as denture stomatitis, halitosis, and caries. Plaque matrix is mostly formed by protein (30%) and polysaccharide complexes. Thus, an alternative enzyme solution as denture cleanser is required for hydrolysis of denture plaque. Papain is a proteolytic enzyme hydrolyzing proteins, while β-1,3-glucanase is a hydrolase enzyme hydrolyzing polysaccharides. Purpose: This study aimed to analyze the differences of effectiveness of ß-1,3- glucanase Vigna unguiculata enzyme and papain Carica papaya enzyme in hydrolysis of denture plaque. Method: This research was a laboratory experimental research with post test only control group design. After using denture for 24 hours, the denture was soaked in a solution of 100 ml PBS, papain enzyme, and β 1-3 glucanase enzyme at a concentration of 0.5 mg/ml, 1 mg/ml, and 2 mg/ml for 10 minutes. The solution from plaque hydrolysis was soaked in PBS and vortex enzyme for 2 minutes, then soaked in ice water for 15 minutes, and centrifuged at 3000 rpm 5-10º for 10 minutes. The supernatant was separated and analyzed. Turbidity readings then were performed in spectrofotometer with a wavelength of 480 nm. Result: 2 mg/ml of ß-1,3 glucanase enzyme generated the highest values of hydrolysis with a mean percentage of 68.77% compared to papain enzyme (44.86 %). The lowest values of hydrolysis weregenerated by PBS with a mean percentage of 3.24%. Conclusion: ß-1,3-glucanase enzyme is more effective in hydrolysis of denture plaque than papain enzyme.

Keywords


Papain; ß-1,3-glucanase; denture plaque

Full Text:

PDF

References


Balitbang Kemenkes RI. Riset Kesehatan Dasar; RISKESDAS. Jakarta: Balitbang Kemenkes RI; 2013. p. 19.

Agtini MD. Percentage of the artificial denture usage in Indonesia. Media Litbang Kesehatan 2010; XX (2): 50–8.

Vargas CM, Kramarow EA, Yellowitz JA. The oral health of older Americans, aging trends. 3rd ed. National Center for Health Statistics; 2001. p. 1.

Craig RG, Powers JM, Wataha JC. Dental materials: properties and Manipulation. USA: Elsavier; 2004. p. 264.

Wahyuningtyas E. Pengaruh ekstrak Graptophyllum Pictum terhadap pertumbuhan Candida abicans pada plat gigi tiruan resin akrilik. Skripsi. Yogyakarta: UGM; 2008. p. 47.

Gharechahi M, Moosavi H, Forghani M. Effect of surface roughness and materials composition on biofilm formation. Journal of Biomaterials and Nanobiotechnology 2012; 3(4A): 541-6.

Rebouillat S, Fernand.P. recent strategies for the development of biosourced-monomers, oligomers and polymers-based materials: “a review with an innovation and a bigger data focus. Journal of Biomaterials and Nanobiotechnology 2016; 07(04): 167-213.

Shay K. Denture hygiene. The Journal of Contemporery Dental Practice 2000; 1(2): 11-7.

Mandali G, Sener ID, Turker SB, Ulgen H. Factors affecting the distribution and prevalence of oral mucosal lesions in complete denture wearers. Gerodontology 2011; 28(2): 97-103.

Coulthwaite L, Verran J. Review article: potential pathogenic aspects of denture plaque. British Journal of Medical Science 2007; 64(4): 181.

Naeem A, Amrit R, Sumit M, Nisha S, Pankaj K, Taseer B. Denture hygiene: a short note on denture cleansers. Journal of Science 2015; 5(3): 131-3.

Carranza FA, Newman MG, Takei H.H. Carranza’s clinical periodontology. 11th ed. St. Louis, Missouri: Saunders El Sevier; 2012. p. 239-48.

Jagadeesh JS, Shalini N. An Overview of Carica papaya and its Medicinal uses. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2014; 5(2): 641.

Sunarintyas S. Peran papain pada pelepasan plak gigi tiruan serta sifat biokompatibilitas. Disertation. Surabaya: Program Pascasarjana Universitas Airlangga; 2002. p. 25, 95, 97, 129-30.

Katatny MHEI, Somitsch W, Robra KH, Katatny MSEI, Gubitz GM. Production of chitinase and 1,3-glucanase by Trichoderma harzianum for control of the Phytopathogenic fungus Sclerotium rolfsii. J Food Technol Biotechnol 2000; 38(3): 170-80.

Afrilliana F, Baktir A, Harsini M. Karakterisasi enzim β-1,3-glukanase dari beberapa sereallia Serta amobilisasi ekstrak kasar enzim terbaik sebagai Kandidat biosensor kandidiasis. Skripsi. Surabaya: Fakultas Sains dan Teknologi Universitas Airlangga; 2014.

Putri MH, Herijulianti E, Nurjannah N. Ilmu pencegahan penyakit jaringan keras dan jaringan pendukung gigi. Preventive dentistry. Jakarta: Penerbit Buku Kedokteran EGC; 2010. p. 257.

Eley BM, Manson JD. Periodontics. 5th edition. London: Elsevier Ltd; 2004. p. 21-8, 133-40.

Amri E, Mamboya F. Papain, a plant enzyme of biological importance: a review. American Journal of Biochemistry and Biotechnology 2012; 8(2): 99-104.

Varghese NJ, Garret TPL, Colman PM, Hou PB, Fincher GB. Three dimensional structures of two plant β-glucan endohydrolases with distinct substrate specifities. Proc Natl Acad Science USA 1994; 91(7): 2785-9.

Xian M, Chen X, Liu Z, Wang K, Wang PG. Inhibition of papain by s-nitrosothiols. Formation of mixed disulfides. J Biol Chem 2000; 275(27): 20467-73.

Wang L, Sun N, Terzyan S, Zhang X, Benson DR. Histidine/ tryptophan π-stacking interaction stabilizes the heme-independent folding core of microsomal apocytochrome b5 relative to that of mitochondrial apocytochrome b5. Biochemistry 2006; 45(46): 13750-9.

Chamani J, Heshmati M, Rajabi O, Parivar K. Thermodynamic study of intermediate state of papain induced by n-alkyl sulfates at two different ph values: A spectroscopic approach. Open Sur Sci J 2009; (1): 20-9.

Cury JA, Rebelo MA, Del Bel Cury AA, Derbyshire MT, Tabchoury CP. Biochemical compositin and cariogenicity of dental plaque formed in the presence of sucrose or glucose and fructose. Caries Res 2000; 34(6): 491-7.

Gafar M, Grigorescu G, Andrescu C, Dumitriu H, Petrescu L, Farcaşiu M, Popescu R, Maliţa C. A study of the microelements in the dental plaque. Rev Chir Oncol Radiol ORL Oftalmol Stomatol Ser Stomatol 1989; 36(3): 161-6.

Harris ELV. Concentrition of extract. In: Harris ELV, Angal S, eds. Protein purification methods: a practical approach. New York: Oxford University IRL. Press; 1998. p. 123–61.


Refbacks

  • There are currently no refbacks.


View My Stats