The effect of Avocado leaf extract (Persea americana Mill.) on the fibroblast cells of post-extraction dental sockets in Wistar rats

Christian Khoswanto, Wisnu Setyari Juliastuti, Karina Awanis Adla

Abstract views = 252 times | views = 171 times


Background: Tooth extraction, a common practice among the dental profession, causes trauma to the blood vessels during the wound healing process. The acceleration of wound healing, within which fibroblasts play an important role, is influenced by nutrition. Avocado leaves contain a variety of chemicals, including flavonoid compounds, tannins, katekat, kuinon, saponin and steroids/triterpenoid. Avocado leaves also contain glycosides, cyanogenic, alkaloids and phenols which function as anti-inflammatory, antibacterial and antioxidant agents. This avocado leaf content could be used as an alternative medicine to accelerate the wound healing process in post-tooth extraction sockets. Purpose: To determine the role of avocado leaves (Persea americana Mill) in accelerating fibroblast cells proliferation in tooth socket post-extraction. Methods: The sample was divided into four groups, a control group and three treatment groups. The treatment groups used avocado leaf extract and 3% CMC Na solution which was inserted into the tooth sockets of Wistar rats. Both the control and treatment groups had their mandibula decapitated with all the required specimens being prepared on the 3rd and 7th days of the experiment. Mandibular decapitation and tooth extraction socket were prepared by HPA (Histology Pathology Anatomy) with Hematoxylin Eosin (HE) staining. The  fibroblast proliferation was analyzed by means of a light microscope at 400x magnification. The obtained data was analyzed using a t-Test. Result: The t-Test obtained a significance value 0.001 (p <0.05) between the control and treatment groups. The number of fibroblast cells increased in the group treated on the third day and decreased in the group treated on the seventh day. Conclusion: Avocado leaf extract (Persea americana Mill.) accelerates proliferation of fibroblast cells in Wistar rats post-tooth extraction.


avocado leafs extract; wound healing; fibroblast

Full Text:



Ardiana T, Kusuma APK, Firdausy MD. Efektivitas pemberian gel binahong (Adredera Cordifolia) 5% terhadap jumlah sel fibroblast pada soket pasca pencabutan gigi marmut (Cavia Cobaya). ODONTO Dent J. 2015; 2: 64–70.

Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M. Growth factors and cytokines in wound healing. Wound Repair Regen. 2008; 16(5): 585–601.

Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 2004; 9: 283–9.

Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010; 89(3): 219–29.

Guyton AC, Hall JE. Guyton dan Hall buku ajar fisiologi kedokteran. 11th ed. Jakarta: EGC; 2008. p. 480-1.

Hargreaves KM, Goodis HE. Seltzer and Bender’s Dental Pulp. Chicago: Quintessence Publishing; 2002. p. 13, 63-4.

Hartono FA, Prabowo PB, Revianti S. Aplikasi gel kitosan berat molekul tinggi dan rendah terhadap ketebalan epitel mukosa pada proses penyembuhan luka pencabutan gigi. Dent J Kedokt Gigi. 2015; 9: 1–10.

Ibsen OAC, Phelan JA. Oral pathology for the dental hygienist. 5th ed. Philadelphia: Elsevier Saunders; 2009. p. 88-91.

Irawati NAV. Antihypertensive effects of avocado leaves extract (Persea americana mill). Majority. 2015; 4: 44–8.

Khoswanto C, Soehardjo I. The effect of Binahong Gel (Anredera cordifolia (Ten.) Steenis) in accelerating the escalation expression of HIF-1α and FGF-2. J Int Dent Med Res. 2018; 11(1): 303–7.

Arciero J, Swigon D. Equation-based models of wound healing and collective cell migration. In: Complex systems and computational biology approaches to acute inflammation. New York: Springer; 2013. p. 185–207.

Krinke G. The laboratory rat. New York: Academic Press; 2000. p. 45-50, 295-6.

Neagos D, Mitran V, Chiracu G, Ciubar R, Iancu C, Stan C, Cimpean A, Iordachescu D. Skin wound healing in a free floating fibroblast poplated collagen lattice model. Rom J Biophys. 2006; 16(3): 157–68.

Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. Flavonoids : a review of probable mechanism of action and potential applications. Am J Clin Nutr. 2001; 74(4): 418–25.

Orsted HL, Keast D, Forest-Lalande L, Françoise M. Basic Principles of Wound Healing An understanding of the basic physiology of wound healing provides. Wound Care Canada. 2012; 9(2): 4–13.

Putra ATW, Ade W, Hamidy MY. Tingkat kepadatan fibroblas pada luka sayat mencit dengan pemberian gel lidah buaya (Aloe chinensis Baker). 2013; : 1–8.

Rowe RC, Sheskey PJ, Owen SC, American Pharmacists Association. Handbook of pharmaceutical excipients. 6th ed. USA: Pharmaceutical Press; 2009. p. 918.

Sheikh AA, Sayyed Z, Siddiqui AR, Pratapwar AS, Sheakh SS. Wound healing activity of Sesbania grandiflora Linn flower ethanolic extract using excision and incision wound model in wistar rats. Int J PharmTech Res. 2011; 3(2): 895–8.


  • There are currently no refbacks.

View My Stats