The comparative Micro-CT analysis on trabecular bone density between hydroxyapatite gypsum puger scaffold application and bovine hydroxyapatite scaffold application
Downloads
Background: Generally, after tooth extraction, trauma is caused by bone damage, which leads to a decreased bone density. Bone damage repair should be conducted using a bone graft containing hydroxyapatite (HA). HA can be synthesised from gypsum puger powder, which is abundant and easy to obtain. Hydroxyapatite gypsum puger (HAGP) was successful with 100% hydroxyapatite purity level. Purpose: To compare the ratio of trabecular bone density in Wistar rats between HAGP scaffold application and bovine hydroxyapatite (BHA) scaffold application. Methods: This study is a laboratory experiment using 6 treatment groups, namely K (-) polyethylene glycol (PEG) 7, K (-) PEG 28, HAGP + PEG 7, HAGP + PEG 28, BHA + PEG 7, and BHA + PEG 28. HAGP scaffold freeze-drying. The rats were anaesthetised intramuscularly, and their left mandibular incisor was removed. The scaffold was applied to the mouse socket, followed by tissue decapitation after 7 and 28 days. The examination was carried out with micro-computed tomography (Micro-CT). Next, statistical analysis using a one-way analysis of variance (ANOVA) test was conducted (p <0.05). Results: The ANOVA test result showed a difference in bone density between the treatment and control groups on days 7 and 28. The Least Significant Difference (LSD) test result revealed that there was no significant difference between K (-) PEG 28 and HAGP + PEG 7 (p=0.133). Nevertheless, there were significant differences between the other groups. Conclusion: Based on the Micro-CT analysis, the trabecular bone density in Wistar rats following HAGP scaffold application is higher than that of BHA scaffold application.
Downloads
Lita YA, Azhari A, Firman RN, Epsilawati L, Pramanik F. Aspek radiografis dan biologis tulang dalam penilaian kualitas tulang pada osteoporosis. J Radiol Dentomaksilofasial Indones. 2019; 3(2): 47–9.
Meyers MA, Chen PY, Lopez MI, Seki Y, Lin AYM. Biological materials: A materials science approach. J Mech Behav Biomed Mater. 2011; 4(5): 626–57.
Sadat-Shojai M, Khorasani MT, Dinpanah-Khoshdargi E, Jamshidi A. Synthesis methods for nanosized hydroxyapatite with diverse structures. Acta Biomater. 2013; 9(8): 7591–621.
Naini A, Rachmawati D. Composition analysis of calcium and sulfur on gypsum at the Puger District Jember Regency as an alternative gypsum dental material. Dentika Dent J. 2010; 15(2): 179–83.
Naini A, Sudiana IK, Rubianto M, Ferdiansyah, Mufti N. Characterization and degradation of hydroxyapatite gypsum puger (HAGP) freeze dried scaffold as a graft material for preservation of the alveolar bone socket. J Int Dent Med Res. 2018; 11(2): 532–6.
Naini A, Sudiana IK, Rubianto M, Kresnoadi U, Latief FDE. Effects of hydroxyapatite gypsum puger scaffold applied to rat alveolar bone sockets on osteoclasts, osteoblasts and the trabecular bone area. Dent J (Majalah Kedokt Gigi). 2019; 52(1): 13–7.
Veni MAC, Rajathi P. Interaction between bone cells in bone remodelling. J Acad Dent Educ. 2: 1–6.
Sims NA, Martin TJ. Coupling signals between the osteoclast and osteoblast: How are messages transmitted between these temporary visitors to the bone surface? Front Endocrinol (Lausanne). 2015; 6: 1–5.
Feng X, McDonald JM. Disorders of bone remodeling. Annu Rev Pathol Mech Dis. 2011; 6: 121–45.
Azhari A, Suprijanto S, Prafiadi H, Juliastuti E. Analisis kemampuan citra radiografi panoramik dalam mendeteksi kerapatan trabekula tulang dengan Micro CT sebagai baku standard -- Image analysis capability of detectinc panoramic radiographic trabecular bone density as standard with standard Micro CT. Indones J Appl Sci. 2014; 4(1): 1–5.
Kusumawati D. Bersahabat dengan hewan coba. Yogyakarta: Gadjah Mada University Press; 2004. p. 5–22.
Latief FDE, Sari DS, Fitri LA. Applications of Micro-CT scanning in medicine and dentistry: Microstructural analyses of a Wistar Rat mandible and a urinary tract stone. J Phys Conf Ser. 2017; 884: 012042.
Rochmatulloh AK, Fawziah UZ, Sumaryono RF, Feranie S, Latief FDE. Analisis citra digital untuk sampel batuan menggunakan Micro-CT Scanner Skyscan 1173. In: Prosiding Seminar Nasional Fisika (E-Journal) SNF2017 UNJ. Pendidikan Fisika dan Fisika FMIPA UNJ; 2017. p. 81–8.
Moisenovich MM, Arkhipova AY, Orlova AA, Drutskaya MS, Volkova S V, Zacharov SE, Agapov II, Kirpichnikov MP. Composite scaffolds containing silk fibroin, gelatin, and hydroxyapatite for bone tissue regeneration and 3D cell culturing. Acta Naturae. 2014; 6(1): 96–101.
Cosman F, de Beur SJ, LeBoff MS, Lewiecki EM, Tanner B, Randall S, Lindsay R, National Osteoporosis Foundation. Clinician's guide to prevention and treatment of osteoporosis. Osteoporos Int. 2014; 25(10): 2359–81.
Lü L-X, Zhang X-F, Wang Y-Y, Ortiz L, Mao X, Jiang Z-L, Xiao Z-D, Huang N-P. Effects of hydroxyapatite-containing composite nanofibers on osteogenesis of mesenchymal stem cells in vitro and bone regeneration in vivo. ACS Appl Mater Interfaces. 2013; 5(2): 319–30.
Jang SJ, Kim SE, Han TS, Son JS, Kang SS, Choi SH. Bone regeneration of hydroxyapatite with granular form or porous scaffold in canine alveolar sockets. In Vivo. 2017; 31(3): 335–41.
Kim JM, Son JS, Kang SS, Kim G, Choi SH. Bone regeneration of hydroxyapatite/alumina bilayered scaffold with 3 mm passage-like medullary canal in canine tibia model. Biomed Res Int. 2015; 2015: 1–6.
da Silva HM, de Lima IR, Bezerra PGP, Peregrino G, de Almeida Soares GD. In vitro dynamic degradation of strontium-hydroxyapatite granules. Key Eng Mater. 2011; 493–494: 205–8.
Sumathi S, Gopal B. In vitro degradation of multisubstituted hydroxyapatite and fluorapatite in the physiological condition. J Cryst Growth. 2015; 422: 36–43.
Wang Y, Yang X, Gu Z, Qin H, Li L, Liu J, Yu X. In vitro study on the degradation of lithium-doped hydroxyapatite for bone tissue engineering scaffold. Mater Sci Eng C. 2016; 66: 185–92.
Lin WC, Chuang CC, Yao C, Tang CM. Effect of cobalt precursors on cobalt-hydroxyapatite used in bone regeneration and MRI. J Dent Res. 2020; 99(3): 277–84.
Tanaka H, Mine T, Ogasa H, Taguchi T, Liang CT. Expression of RANKL/OPG during bone remodeling in vivo. Biochem Biophys Res Commun. 2011; 411(4): 690–4.
- Every manuscript submitted to must observe the policy and terms set by the Dental Journal (Majalah Kedokteran Gigi).
- Publication rights to manuscript content published by the Dental Journal (Majalah Kedokteran Gigi) is owned by the journal with the consent and approval of the author(s) concerned.
- Full texts of electronically published manuscripts can be accessed free of charge and used according to the license shown below.
- The Dental Journal (Majalah Kedokteran Gigi) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
